Manipulation of triacylglycerol biosynthesis in Nannochloropsis oceanica by overexpressing an Arabidopsis thaliana diacylglycerol acyltransferase gene

Algal Research - Tập 61 - Trang 102590 - 2022
Lin Zhang1, Song Wang2, Ji-Chang Han3,4, Guan-Pin Yang4, Ke-Hou Pan5,6, Ji-Lin Xu1
1Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education of China, Ningbo, Zhejiang 315211, China
2College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China
3College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang 315211, China
4College of Marine Life Science, Ocean University of China, Qingdao, Shandong 266003, China
5Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China
6Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China

Tài liệu tham khảo

Faried, 2017, Biodiesel production from microalgae: processes, technologies and recent advancements, Renew. Sustain. Energy Rev., 79, 893, 10.1016/j.rser.2017.05.199 Sibi, 2016, Enhanced lipid productivity approaches in microalgae as an alternate for fossil fuels – a review, J. Energy Inst., 89, 330, 10.1016/j.joei.2015.03.008 Kadir, 2018, Harvesting and pretreatment of microalgae cultivated in wastewater for biodiesel production: a review, energ. ConversManage, 171, 1416 Sun, 2018, Novel insight of carotenoid and lipid biosynthesis and their roles in storage carbon metabolism in Chlamydomonas reinhardtii, Bioresour. Technol., 263, 450, 10.1016/j.biortech.2018.05.035 Xiao, 2015, Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions, Algal Res., 11, 55, 10.1016/j.algal.2015.05.019 Zienkiewicz, 2017, Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts, Biotechnol. Biofuels, 10, 8, 10.1186/s13068-016-0686-8 Liu, 2021, Novel insights into type 2 diacylglycerol acyltransferases in microalga Myrmecia incisa, J. Appl. Phycol., 33, 25, 10.1007/s10811-020-02071-x Wei, 2017, A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica, Biotechnol. Biofuels, 10, 174, 10.1186/s13068-017-0858-1 Michaud, 2017, Glycerolipid synthesis and lipid trafficking in plant mitochondria, FEBS J., 284, 376, 10.1111/febs.13812 Manandhar-Shrestha, 2015, Characterization and manipulation of a DGAT2 from the diatom Thalassiosira pseudonana: improved TAG accumulation without detriment to growth, and implications for chloroplast TAG accumulation, Algal Res., 12, 239, 10.1016/j.algal.2015.09.004 Niu, 2013, Improvement of neutral lipid and polyunsaturated fatty acid biosynthesis by overexpressing a type 2 diacylglycerol acyltransferase in marine diatom Phaeodactylum tricornutum, Mar. Drugs, 11, 4558, 10.3390/md11114558 Ahmad, 2015, Altered lipid composition and enhanced lipid production in green microalga by introduction of brassica diacylglycerol acyltransferase 2, Plant Biotechnol. J., 13, 540, 10.1111/pbi.12278 Andrianov, 2010, Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass, Plant Biotechnol. J., 8, 277, 10.1111/j.1467-7652.2009.00458.x Guo, 2019, Genome assembly of Nannochloropsis oceanica provides evidence of host nucleus overthrow by the symbiont nucleus during speciation, Commun. Biol., 2, 249, 10.1038/s42003-019-0500-9 Ma, 2016, Physiological and biochemical changes reveal stress-associated photosynthetic carbon partitioning into triacylglycerol in the oleaginous marine alga Nannochloropsis oculata, Algal Res., 16, 28, 10.1016/j.algal.2016.03.005 Wang, 2016, Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9, Plant J., 88, 1071, 10.1111/tpj.13307 Wei, 2017, RNAi-based targeted gene knockdown in the model oleaginous microalgae Nannochloropsis oceanica, Plant J., 89, 1236, 10.1111/tpj.13411 Li, 2016, A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica, J. Biotechnol., 229, 65, 10.1016/j.jbiotec.2016.05.005 Han, 2019, Enhancing the lipid content of Nannochloropsis oceanica by introducing two type 2 diacylglycerol acyltransferase genes from Phaeodactylum tricornutum, J. Biobased Mater. Bioenergy, 13, 1, 10.1166/jbmb.2019.1859 Guillard, 1962, Studies of marine plankton diatoms. I. Cyclotella nana (Hustedt), and Detonula confervacea (Cleve) Gran, Can. J. Microbiol., 8, 229, 10.1139/m62-029 Porebski, 1997, Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components, Plant Mol. Biol. Rep., 15, 8, 10.1007/BF02772108 Li, 2009, Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract, Fish Shellfish Immun., 26, 316, 10.1016/j.fsi.2008.07.004 Zhang, 2020, Identification and functional characterization of a novel Δ12 fatty acid desaturase gene from Haematococcus pluvialis, J. Ocean Univ. China, 19, 1362, 10.1007/s11802-020-4418-0 Bligh, 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37, 911, 10.1139/y59-099 Wang, 2016, Breeding 3 elite strains of Nannochloropsis oceanica by nitrosoguanidine mutagenesis and robust screening, Algal Res., 19, 104, 10.1016/j.algal.2016.07.021 Xin, 2017, Producing designer oils in industrial microalgae by rational modulation of co-evolving type-2 diacylglycerol acyltransferases, Mol. Plant, 10, 1523, 10.1016/j.molp.2017.10.011 Zienkiewicz, 1861, Stress-induced neutral lipid biosynthesis in microalgae—molecular, cellular and physiological insights, BBA-Mol. Cell Biol. Lipids, 2016, 1269 McKeon, 2015, Castor diacylglycerol acyltransferase type 1 (DGAT1) displays greater activity with diricinolein than Arabidopsis DGAT1, biocatal. AgricBiotechnol., 4, 276, 10.1016/j.bcab.2015.01.005 Liu, 2016, Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis, Plant J., 86, 3, 10.1111/tpj.13143 Fan, 2014, Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors, Biotechnol. Biofuels, 7, 17, 10.1186/1754-6834-7-17 Wang, 2014, Nannochloropsis genomes reveal evolution of microalgal oleaginous traits, PLoS Genet., 10, 10.1371/journal.pgen.1004094 Iwai, 2014, Enhancement of extraplastidic oil synthesis in Chlamydomonas reinhardtii using a type-2 diacylglycerol acyltransferase with a phosphorus starvation-inducible promoter, Plant Biotechnol. J., 12, 808, 10.1111/pbi.12210 Kehelpannala, 2021, An arabidopsis lipid map reveals differences between tissues and dynamic changes throughout development, Plant J., 10.1111/tpj.15278 Aymé, 2014, Function and localization of the Arabidopsis thaliana diacylglycerol acyltransferase DGAT2 expressed in yeast, PLoS One, 9, 10.1371/journal.pone.0092237 Bouvier-Navé, 2000, Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglycerol acyltransferase, Eur. J. Biochem., 267, 85, 10.1046/j.1432-1327.2000.00961.x Jako, 2001, Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight, Plant Physiol., 126, 861, 10.1104/pp.126.2.861 Watts, 2021, Optimizing protein expression in heterologous system: strategies and tools, Meta Gene, 29, 10.1016/j.mgene.2021.100899 Ohlrogge, 1995, Lipid biosynthesis, Plant Cell, 7, 957 Amack, 2020, CaMV35S promoter – a plant biology and biotechnology workhorse in the era of synthetic biology, Curr. Plant Biol., 24, 10.1016/j.cpb.2020.100179 Schrodal, 2000, The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas, Plant J., 21, 121, 10.1046/j.1365-313x.2000.00652.x Ma, 2016, Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker, J. Ocean Univ. China, 15, 351, 10.1007/s11802-016-2715-4 Kirchhoff, 2020, Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines, Plant Biotechnol. Rep., 14, 451, 10.1007/s11816-020-00624-7