Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals

International Journal of Systematic and Evolutionary Microbiology - Tập 54 Số 2 - Trang 623-627 - 2004
Ayako Hirai1, Rui Kano1, Koichi Makimura2, Eduardo Robson Duarte3, Júnia Soares Hamdan3, Marc‐André Lachance4, Hideyo Yamaguchi2, Atsuhiko Hasegawa1
1Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Fujisawa, Kanagawa 252-8510, Japan
2Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan
3Department of Microbiology, Biological Science Institute, Federal University of Minas Gerais, Avenida Antonio Carlos, Belo Horizonte, Minas Gerais, 31270-901, Brazil
4Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7

Tóm tắt

Five isolates of a novel species of the yeast genus Malassezia were isolated from animals in Japan and Brazil. Phylogenetic trees based on the D1/D2 domains of the large-subunit (26S) rDNA sequences and nucleotide sequences of the internal transcribed spacer 1 region showed that the isolates were conspecific and belonged to the genus Malassezia. They were related closely to Malassezia dermatis and Malassezia sympodialis, but were clearly distinct from these two species and the other six species of Malassezia that have been reported, indicating that they should be classified as a novel species, Malassezia nana sp. nov. Morphologically and physiologically, M. nana resembles M. dermatis and M. sympodialis, but can be distinguished from these species by its inability to use Cremophor EL (Sigma) as the sole lipid source and to hydrolyse aesculin. The type strain of M. nana is NUSV 1003T (=CBS 9557T=JCM 12085T).

Từ khóa


Tài liệu tham khảo

Aizawa, 1999, Molecular heterogeneity in clinical isolates of Malassezia pachydermatis from dogs, Vet Microbiol, 70, 67, 10.1016/S0378-1135(99)00126-1

Aizawa, 2001, The genetic diversity of clinical isolates of Malassezia pachydermatis from dogs and cats, Med Mycol, 39, 329, 10.1080/mmy.39.4.329.334

Ashbee, 2002, Immunology of diseases associated with Malassezia species, Clin Microbiol Rev, 15, 21, 10.1128/CMR.15.1.21-57.2002

Boekhout, 1998, Diagnostic descriptions and key to presently accepted heterobasidiomycetous genera. In The Yeasts , a Taxonomic Study, p

Boekhout, 1998, Molecular typing of Malassezia species with PFGE and RAPD, Med Mycol, 36, 365, 10.1080/02681219880000581

Crespo, 2000, Otitis externa associated with Malassezia sympodialis in two cats, J Clin Microbiol, 38, 1263, 10.1128/JCM.38.3.1263-1266.2000

Duarte, 1999, Prevalence of Malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in Brazil, Med Mycol, 37, 159, 10.1080/j.1365-280X.1999.00212.x

Duarte, 2001, Prevalence of yeasts and mycelial fungi in bovine parasitic otitis in the state of Minas Gerais, Brazil. J Vet Med B Infect Dis Vet Public Health, 48, 631, 10.1046/j.1439-0450.2001.00474.x

Fell, 2000, Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis, Int J Syst Evol Microbiol, 50, 1351, 10.1099/00207713-50-3-1351

Felsenstein, 1985, Confidence limits on phylogenies: an approach using the bootstrap, Evolution, 39, 783, 10.1111/j.1558-5646.1985.tb00420.x

Gaitanis, 2002, Identification of Malassezia species from patient skin scales by PCR-RFLP, Clin Microbiol Infect, 8, 162, 10.1046/j.1469-0691.2002.00383.x

Gemmer, 2002, Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff microbiology, J Clin Microbiol, 40, 3350, 10.1128/JCM.40.9.3350-3357.2002

Greene, 1998, Clinical problems. In Infectious Diseases of the Dog and Cat, 549

Guého, 1996, The genus Malassezia with description of four new species, Antonie van Leeuwenhoek, 69, 337, 10.1007/BF00399623

Guillot, 1995, The diversity of Malassezia yeasts confirmed by rRNA sequence and nuclear DNA comparisons, Antonie van Leeuwenhoek, 67, 297, 10.1007/BF00873693

Gupta, 2000, Molecular differentiation of seven Malassezia species, J Clin Microbiol, 38, 1869, 10.1128/JCM.38.5.1869-1875.2000

Hagler, 1981, Rapid diazonium blue B test to detect basidiomycetous yeasts, Int J Syst Bacteriol, 31, 204, 10.1099/00207713-31-2-204

Hammer, 2000, Precipitate production by some Malassezia species on Dixon's agar, Med Mycol, 38, 105, 10.1080/mmy.38.2.105.107

Hirai, 2002, A unique isolate of Malassezia from a cat, J Vet Med Sci, 64, 957, 10.1292/jvms.64.957

Kano, 1999, Chitin synthase 2 gene sequence of Malassezia species, Microbiol Immunol, 43, 813, 10.1111/j.1348-0421.1999.tb02475.x

Lachance, 1999, Kodamaea kakaduensis and Candida tolerans , two new ascomycetous yeast species from Australian Hibiscus flowers, Can J Microbiol, 45, 172, 10.1139/w98-225

Makimura, 2000, Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions, J Med Microbiol, 49, 29, 10.1099/0022-1317-49-1-29

Mayser, 1997, Differentiation of Malassezia species: selectivity of cremophor EL, castor oil and ricinoleic acid for M. furfur, Br J Dermatol, 137, 208, 10.1046/j.1365-2133.1997.18071890.x

Midgley, 2000, The lipophilic yeasts: state of the art and prospects, Med Mycol, 38, 9, 10.1080/mmy.38.s1.9.16

Page, 1996, treeview: an application to display phylogenetic trees on personal computers, Comput Appl Biosci, 12, 357

Scorzetti, 2002, Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions, FEMS Yeast Res, 2, 495, 10.1016/S1567-1356(02)00128-9

Senczek, 1999, Characterization of Malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulse-field gel electrophoresis (PFGE, Mycoses, 42, 409, 10.1046/j.1439-0507.1999.00478.x

Sugita, 2002, New yeast species, Malassezia dermatis , isolated from patients with atopic dermatitis, J Clin Microbiol, 40, 1363, 10.1128/JCM.40.4.1363-1367.2002

Theelen, 2001, Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLPTM), random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE, FEMS Yeast Res, 1, 79, 10.1111/j.1567-1364.2001.tb00018.x

Thompson, 1994, clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res, 22, 4673, 10.1093/nar/22.22.4673

Thông tin
Thông tin xuất bản

International Journal of Systematic and Evolutionary Microbiology

Tập 54 Số 2

623-627

Thông tin tác giả