Macrofagi e risposta immunitaria. I. effetti del blocco dei macrofagi peritoneali sulla risposta immunitaria primaria in topi BALB/c. studio istologico ed immunologico in funzione del tempo di somministrazione dell’agente bloccante
Tóm tắt
Nel corso del presente esperimento abbiamo studiato gli effetti di un’unica somministrazione intraperitoneale di particelle di carbone colloidale, vale a dire di un agente ingolfante i macrofagi, sul quadro istologico e sulla reattività immunitaria primaria ai G.R.M., nel topo di ceppo BALB/c. Il carbone colloidale è stato iniettato a diversi intervalli di tempo prima, contemporaneamente o dopo l’immunizzazione. I risultati indicano che il trattamento con carbone deprime la risposta immunitaria primaria solo se questo è stato somministrato a brevi intervalli prima dell’antigene. Di conseguenza i macrofagi sembrano giocare un ruolo molto importante negli eventi cellulari associati alla risposta immunitaria primaria ai G.R.M. Appare anche evidente dalle riportate osservazioni che l’interazione macrofago-antigene avviene effettivamente e precede l’interazione fra T-linfocita ed antigene. Inoltre si ritiene di poter suggerire che i macrofagi peritoneali costituiscano un terzo tipo cellulare necessario per l’instaurarsi della risposta immunitaria primaria ad alcuni antigeni particolati somministrati per via peritoneale.
Tài liệu tham khảo
Adler F. L., Fishman M., Dray S.: Antibody Formation Initiated in Vitro. III. Antibody Formation and Allotypic Specificity Directed by Ribonucleic Acid from Peritoneal Exudate Cells - J. Immunol.97, 554, 1966.
Argyris B. F.: Role of Macrophages in Antibody Production: Immune Response to Sheep Red Blood Cells - J. Immunol.99, 744, 1967.
Argyris B. F., Plotkin D. H.: Effects of Antimacrophage, Antithymocyte, Antilymphocyte and Antispleen Serum on the Immune Response in Mice - Clin. exp. Immunol.7, 551, 1970.
Askonas B. A., Rhodes J. M.: Immunogenicity of Antigen-Containing Ribonucleic Acid Preparations from Macrophages - Nature (Lond.)205, 470, 1965.
Fisher S.: Stimulation of Splenic Antigen Uptake and of Antibody Response in Mice by India Ink or Other ≪Blockading≫ Agents - Immunology11, 127, 1966.
Fishman M., Adler F. L.: Antibody Formation Initiated in Vitro. II. Antibody Synthesis in X-Irradiated Recipients of Diffusion Chambers Containing Nucleic Acid Derived from Macrophages Incubated with Antigen - J. exp. Med.117, 595, 1963.
Gottleib A. A.: Macrophage Ribonucleoprotein: Nature of the Antigenic Fragment - Science165, 592, 1969.
Hersh E. M., Harris J. E.: Macrophage-Lymphocyte Interaction in the Antigen-Induced Blastogenic Response of Human Peripheral Leukocytes - J. Immunol.100, 1184, 1968.
Hirsch J. G.: Phagocytosis - Ann. Rev. Microbiol.19, 339, 1965.
Jenkin C. R., Anzin I., Reade P. P.: The Synthesis of Macroglobulin Antibody to a Bacterial Antigen in Mice after Treatment with Thorotrast - Aust. J. exp. Biol. med. Sci.43, 607, 1965.
Jerne N. K., Nordin A. A., Henry C.: The Agar Plaque Technique for Recognizing Antibody-Producing Cells - In:Amos B., Koprowsky H. (Eds): Cell-Bound Antibodies. Winstar Inst. Symp. Monograph, no. 3. Winstar Inst. Press, Philadelphia, 1963; p. 109.
Kölsch E.: Macrophages: Cooperating Cells with a Limited Discrimination for Antigen - In: Antibody Workshop Meeting: ≪ The Structure, Genetics and Formation of Antibodies ≫. N. G. Elvert, Universitäts- und Verlagsbuchhandlung Marburg/Lahn, 1969; p. 196.
Melnick H. D., Friedman H.: The Immunosuppressive Effect of RES ≪Blockade≫ with Ethyl Stearate Emulsion on Cellular Formation of Antibody to Sheep Erythrocytes - Proc. Soc. exp. Biol.133, 423, 1970.
Mitchison N. A.: The Immunogenic Capacity of Antigen Taken up by Peritoneal Exudate Cells - Immunology16, 1, 1969.
Mosier D. E.: A Requirement for Two Cell Types for Antibody Formation in Vitro - Science158, 1573, 1967.
North R. J.: The Relative Importance of Blood Monocytes and Fixed Macrophages to the Expression of Cell-Mediated Immunity to Infection - J. exp. Med.132, 521, 1970.
North R. J.: Suppression of Cell-Mediated Immunity to Infection by an Antimitotic Drug. Further Evidence that Migrant Macrophages Express Immunity - J. exp. Med.132, 535, 1970.
Pierce C. W.: Immune Responses in Vitro. II. Suppression of the Immune Response in Vitro by Specific Antibody - J. exp. Med.130, 365, 1969.
Rowley D.: Phagocytosis - Advanc. Immunol.2, 241, 1962.
Sabet T., Newlin C., Friedman M.: The Effect of RES Blockade on Cellular Antibody Formation to Sheep Erythrocytes - Proc. Soc. exp. Biol.128, 274, 1968.
Sabet T., Newlin C., Friedman H.: Effect of RES ≪ Blockade ≫ on Antibody-Formation. I. Suppressed Cellular and Humoral Haemolysin Responses in Mice Injected with Carbon Particles - Immunology16, 433, 1969.
Seeger R. C., Oppenheim J. J.: Synergistic Interaction of Macrophages and Lymphocytes in Antigen-Induced Transformation of Lymphocytes - J. exp. Med.132, 44, 1970.
Stern K.: Inhibition in Vivo of Reticulo-Endothelial Phagocytosis by Synthetic Polyamino Acids - Proc. Soc. exp. Biol.114, 321, 1966.
Stern K., Spencer K., Farquhar M.: Effect of Polyvynil Pyrrolidone on Immune Response in Mice- Proc. Soc. exp. Biol.89, 126, 1955.
Unanue E. R., Askonas B. A.: The Immune Response of Mice to Antigen in Macrophages - Immunology15, 287, 1968.
Unanue E. R., Cerottini J. C., Bedford M.: Persistence of Antigen on the Surface of Macrophages - Nature (Lond.)222, 1193, 1969.