MEGA11: Molecular Evolutionary Genetics Analysis Version 11

Molecular Biology and Evolution - Tập 38 Số 7 - Trang 3022-3027 - 2021
Koichiro Tamura1,2, Glen Stecher3, Sudhir Kumar4,5,3
1Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan
2Research Center for Genomics and Bioinformatics, Tokyo Metropolitan University, Tokyo, Japan
3Institute for Genomics and Evolutionary Medicine, Temple University, Philadelphia, PA, USA
4Center for Excellence in Genome Medicine and Research, King Abdulaziz University, Jeddah, Saudi Arabia
5Department of Biology, Temple University, Philadelphia, PA, USA

Tóm tắt

Abstract

The Molecular Evolutionary Genetics Analysis (MEGA) software has matured to contain a large collection of methods and tools of computational molecular evolution. Here, we describe new additions that make MEGA a more comprehensive tool for building timetrees of species, pathogens, and gene families using rapid relaxed-clock methods. Methods for estimating divergence times and confidence intervals are implemented to use probability densities for calibration constraints for node-dating and sequence sampling dates for tip-dating analyses. They are supported by new options for tagging sequences with spatiotemporal sampling information, an expanded interactive Node Calibrations Editor, and an extended Tree Explorer to display timetrees. Also added is a Bayesian method for estimating neutral evolutionary probabilities of alleles in a species using multispecies sequence alignments and a machine learning method to test for the autocorrelation of evolutionary rates in phylogenies. The computer memory requirements for the maximum likelihood analysis are reduced significantly through reprogramming, and the graphical user interface has been made more responsive and interactive for very big data sets. These enhancements will improve the user experience, quality of results, and the pace of biological discovery. Natively compiled graphical user interface and command-line versions of MEGA11 are available for Microsoft Windows, Linux, and macOS from www.megasoftware.net.

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