Loss of Layer‐specific Astrocytic Glutamine Synthetase Immunoreactivity in Slice Cultures of Hippocampus

European Journal of Neuroscience - Tập 5 Số 2 - Trang 122-127 - 1993
Amin Derouiche1, Bernd Heimrich2, Michael Frotscher2
1Institute of Anatomy, University of Frankfurt am Main, Germany.
2Institute of Anatomy, University of Freiburg, Albertstrasse 17, 7800 Freiburg, Germany

Tóm tắt

AbstractGlutamine synthetase (GS) supposedly inactivates the excitatory neurotransmitter glutamate. By using immunocytochemistry for GS, we recently demonstrated a layer‐specific, perisynaptic distribution of GS‐immunoreactive astrocytes and their processes in perfusion‐fixed rat hippocampi. Highest levels of immunoreactivity were found in well defined termination zones of glutamatergic hippocampal afferents. In the present study we analysed the developmental aspect of this neuron – glia interaction by using hippocampal slice cultures lacking all extrinsic afferents. Under these conditions, no layer‐specific distribution of astrocytic GS immunoreactivity could be demonstrated. This suggests that the laminated distribution of GS immunoreactivity is formed in parallel with the segregated termination of hippocampal afferents. Thus, there is no predetermined pattern of GS‐containing astrocytes playing a role in the segregation of extrinsic fibres. The ultrastructural localization of GS immunoreactivity in fine astrocytic processes around asymmetric, probably glutamatergic excitatory spine synapses confirms earlier in situ findings, which suggests that this arrangement is a global phenomenon of glutamatergic systems.

Từ khóa


Tài liệu tham khảo

10.1016/0165-3806(83)90049-4

10.1002/glia.440030503

Cotman C. W., 1978, Neuronal Plasticity., 227

10.1016/0306-4522(91)90298-3

10.1016/0006-8993(91)90103-3

10.1007/BF01175194

10.1002/cne.902390210

10.1016/0306-4522(88)90348-X

10.1016/S0079-6123(08)61260-1

10.1016/0165-0270(81)90003-0

10.1016/0306-4522(84)90192-1

10.1007/BF00492479

10.1016/0306-4522(91)90335-L

10.1016/0006-8993(84)90810-2

10.1016/0006-8993(91)90438-2

Hsu S. M., 1981, The use of avidin biotin complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (peroxidase) procedures, J. Histochem., 29, 577

10.1002/cne.902490209

10.1016/0306-4522(86)90112-0

10.1038/342427a0

Schmidt‐Kastner R., 1990, Immunohistochemistry of glial fibrillary acidic protein, vimentin and S 100 protein for study of astrocytes in hippocampus of rat, J. Chem. Neuroanat., 3, 179

10.1007/BF00341419

10.1016/0306-4522(92)90187-7

10.1002/cne.902280310

Thông tin
Thông tin xuất bản

European Journal of Neuroscience

Tập 5 Số 2

122-127

Thông tin tác giả