Long-Term Therapy with a New Chemically Modified Tetracycline (CMT-8) Inhibits Bone Loss in Femurs of Ovariectomized Rats

Advances in dental research - Tập 12 Số 1 - Trang 76-81 - 1998
Takahísa Sasaki1, N S Ramamurthy2, Lorne M. Golub2
1Department of Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
2Department of Oral Biology and Pathology, School of Dental Medicine, Health Sciences Center, State University of New York at Stony Brook, Stony Brook, New York 11794-8700, USA

Tóm tắt

The effect of a new non-antimicrobial analog of tetracycline (CMT-8) on bone loss in ovariectomized (OVX) rats was examined. Three-month-old female rats were ovariectomized, and one week later, were distributed into 3 groups: sham-operated non-OVX controls, vehicle-treated OVX controls, and CMT-8-treated OVX rats. After 145 days of daily CMT-8 administration, the intact femurs were dissected and examined by several histological and histomorphometric techniques. OVX significantly (p < 0.01) decreased trabecular bone volume by 53.4% in the metaphyses compared with sham-operated controls. CMT-8 therapy produced a significant (p < 0.05) inhibition of trabecular bone loss and also induced bone formation in the OVX rats. Of interest, the newly synthesized bone in the CMT-treated OVX rats was found to increase the "connectivity" of the trabecular "struts" by bridging the adjacent longitudinal bone trabeculae, forming dense, platelike bone trabeculae. These results strongly suggest that long-term CMT-8 therapy effectively inhibits bone loss after OVX, not only by inhibiting bone resorption but also by inducing new bone formation in the trabecular areas of long bones.

Từ khóa


Tài liệu tham khảo

10.1007/BF00675623

10.1002/jbmr.5650050310

10.1111/j.1600-0765.1984.tb01334.x

Golub LM, 1990, Res Commun Chem Pathol Pharmacol, 68, 24

10.1007/BF01946671

10.1016/S8756-3282(97)00221-4

10.1016/8756-3282(91)90030-M

10.1016/S0169-6009(08)80037-2

10.1210/endo-122-2-624

10.1016/0169-6009(91)90124-I

10.1016/S0169-6009(08)80050-5

10.1002/ar.1092360303

10.1016/8756-3282(91)90033-F

10.1016/8756-3282(93)90248-9

Ramamurthy NS, 1996, J Dent Res, 75, 105

Rifkin BR, Golub LM, Sanavi F., Vernillo AT, Kleckner AP, McNamara TF, et al. (1992). Effects of tetracyclines on rat osteoblast collagenase activity and bone resorption in vitro. In: The biological mechanisms of tooth movement and craniofacial adaptation. Davidovitch Z, editor. Birmingham, AL : EBSCO Media, pp. 85-90.

10.1111/j.1749-6632.1994.tb24733.x

10.1097/00002281-199605000-00013

Sasaki T., 1990, J Biol Buccale, 18, 215

10.1002/ar.1092310105

10.1007/BF00296771

10.1016/8756-3282(94)90303-4

10.1172/JCI116483

10.1002/jbmr.5650080313

10.1016/S8756-3282(96)00302-X

Wronski TJ, 1991, Cells Mater, 1, 69

10.1016/8756-3282(89)90067-7

Thông tin
Thông tin xuất bản

Advances in dental research

Tập 12 Số 1

76-81

Thông tin tác giả