Localized, non-viral delivery of nucleic acids: Opportunities, challenges and current strategies

Asian Journal of Pharmaceutical Sciences - Tập 10 - Trang 159-175 - 2015
Oliver Germershaus1, Kira Nultsch1
1Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, CH 4132, Muttenz, Switzerland

Tài liệu tham khảo

Xu, 2011, Drug delivery trends in clinical trials and translational medicine: challenges and opportunities in the delivery of nucleic acid-based therapeutics, J Pharm Sci, 100, 38, 10.1002/jps.22243 Hickerson, 2008, Stability study of unmodified sirna and relevance to clinical use, Oligonucleotides, 18, 345, 10.1089/oli.2008.0149 Rohland, 2010, Genomic DNA sequences from mastodon and woolly mammoth reveal deep speciation of forest and savanna elephants, Plos Biol, 8 Ewe, 2014, Storage stability of optimal liposome-polyethylenimine complexes (lipopolyplexes) for DNA or siRNA delivery, Acta Biomater, 10, 2663, 10.1016/j.actbio.2014.02.037 Kundu, 2012, Stability of lyophilized siRNA nanosome formulations, Int J Pharm, 423, 525, 10.1016/j.ijpharm.2011.11.040 Kasper, 2013, Formulation development of lyophilized, long-term stable siRNA/oligoaminoamide polyplexes, Eur J Pharm Biopharm, 85, 294, 10.1016/j.ejpb.2013.05.010 Liemann, 1999, Influence of amino acid substitutions related to inherited human prion diseases on the thermodynamic stability of the cellular prion protein, Biochemistry, 38, 3258, 10.1021/bi982714g Newton, 1998, Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties, Biochemistry, 37, 5173, 10.1021/bi972147h Sah, 2006, Therapeutic potential of RNA interference for neurological disorders, Life Sci, 79, 1773, 10.1016/j.lfs.2006.06.011 Uprichard, 2005, The therapeutic potential of RNA interference, Febs Lett, 579, 5996, 10.1016/j.febslet.2005.08.004 Guo, 2012, Recent advances in nonviral vectors for gene delivery, Acc Chem Res, 45, 971, 10.1021/ar200151m Buyens, 2012, Liposome based systems for systemic siRNA delivery: stability in blood sets the requirements for optimal carrier design, J Control Release, 158, 362, 10.1016/j.jconrel.2011.10.009 Bauhuber, 2009, Delivery of nucleic acids via disulfide-based carrier systems, Adv Mater, 21, 3286, 10.1002/adma.200802453 Gullotti, 2009, Extracellularly activated nanocarriers: a new paradigm of tumor targeted drug delivery, Mol Pharm, 6, 1041, 10.1021/mp900090z Ganta, 2008, A review of stimuli-responsive nanocarriers for drug and gene delivery, J Control Release, 126, 187, 10.1016/j.jconrel.2007.12.017 Bertin, 2014, Polyelectrolyte complexes of DNA and polycations as gene delivery vectors, vol. 256, 103 Germershaus, 2008, Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: establishment of structure-activity relationships in vitro, J Control Release, 125, 145, 10.1016/j.jconrel.2007.10.013 Boussif, 1995, A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo – polyethylenimine, Proc Natl Acad Sci U S A, 92, 7297, 10.1073/pnas.92.16.7297 Mao, 2010, Chitosan-based formulations for delivery of DNA and siRNA, Adv Drug Deliv Rev, 62, 12, 10.1016/j.addr.2009.08.004 Dufes, 2005, Dendrimers in gene delivery, Adv Drug Deliv Rev, 57, 2177, 10.1016/j.addr.2005.09.017 Benjaminsen, 2013, The possible “proton sponge” effect of polyethylenimine (PEI) does not include change in lysosomal pH, Mol Ther, 21, 149, 10.1038/mt.2012.185 Rehman, 2013, Mechanism of polyplex- and lipoplex-mediated delivery of nucleic acids: real-time visualization of transient membrane destabilization without endosomal lysis, ACS Nano, 7, 3767, 10.1021/nn3049494 Petersen, 2002, Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system, Bioconjug Chem, 13, 845, 10.1021/bc025529v Ogris, 1999, PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery, Gene Ther, 6, 595, 10.1038/sj.gt.3300900 Germershaus, 2006, Trastuzumab-polyethylenimine-polyethylene glycol conjugates for targeting Her2-expressing tumors, Bioconjug Chem, 17, 1190, 10.1021/bc0601119 Germershaus, 2008, HER2 targeted polyplexes: the effect of polyplex composition and conjugation chemistry on in vitro and in vivo characteristics, Bioconjug Chem, 19, 244, 10.1021/bc700311n Neu, 2007, Crosslinked nanocarriers based upon poly(ethylene imine) for systemic plasmid delivery: in vitro characterization and in vivo studies in mice, J Control Release, 118, 370, 10.1016/j.jconrel.2007.01.007 Neu, 2007, Bioreversibly crosslinked polyplexes of PEI and high molecular weight PEG show extended circulation times in vivo, J Control Release, 124, 69, 10.1016/j.jconrel.2007.08.009 Florea, 2002, Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures, AAPS PharmSci, 4 vandeWetering, 1997, Relation between transfection efficiency and cytotoxicity of poly(2-(dimethylamino)ethyl methacrylate)/plasmid complexes, J Control Release, 49, 59, 10.1016/S0168-3659(97)00059-X Breunig, 2007, Breaking up the correlation between efficacy and toxicity for nonviral gene delivery, Proc Natl Acad Sci U S A, 104, 14454, 10.1073/pnas.0703882104 Peng, 2008, Disulfide cross-linked polyethylenimines (PEI) prepared via thiolation of low molecular weight PEI as highly efficient gene vectors, Bioconjug Chem, 19, 499, 10.1021/bc7003236 Yin, 2014, Non-viral vectors for gene-based therapy, Nat Rev Genet, 15, 541, 10.1038/nrg3763 Felgner, 1987, Lipofection – a highly efficient, lipid-mediated DNA-transfection procedure, Proc Natl Acad Sci U S A, 84, 7413, 10.1073/pnas.84.21.7413 Konopka, 1991, Enhancement of human-immunodeficiency-virus type-1 infection by cationic liposomes – the role of cd4, serum and liposome cell-interactions, J Gen Virol, 72, 2685, 10.1099/0022-1317-72-11-2685 Felgner, 1994, Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations, J Biol Chem, 269, 2550, 10.1016/S0021-9258(17)41980-6 Zuhorn, 2002, Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis, J Biol Chem, 277, 18021, 10.1074/jbc.M111257200 Dass, 2002, Cytotoxicity issues pertinent to lipoplex-mediated gene therapy in-vivo, J Pharm Pharmacol, 54, 593, 10.1211/0022357021778817 de Ilarduya, 2010, Gene delivery by lipoplexes and polyplexes, Eur J Pharm Sci, 40, 159, 10.1016/j.ejps.2010.03.019 Ozpolat, 2014, Liposomal siRNA nanocarriers for cancer therapy, Adv Drug Deliv Rev, 66, 110, 10.1016/j.addr.2013.12.008 Li, 2006, Targeted delivery of antisense oligodeoxynucleotide and small interference RNA into lung cancer cells, Mol Pharm, 3, 579, 10.1021/mp060039w Garcia, 2012, Lipopolyplexes as nanomedicines for therapeutic gene delivery, vol. 509, 327 Chono, 2008, An efficient and low immunostimulatory nanoparticle formulation for systemic siRNA delivery to the tumor, J Control Release, 131, 64, 10.1016/j.jconrel.2008.07.006 Jain, 2013, Enhanced transfection efficiency and reduced cytotoxicity of novel lipid-polymer hybrid nanoplexes, Mol Pharm, 10, 2416, 10.1021/mp400036w Shen, 2006, Suppression of ocular neovascularization with siRNA targeting VEGF receptor 1, Gene Ther, 13, 225, 10.1038/sj.gt.3302641 Kaiser, 2010, RNAi-based treatment for neovascular age-related macular degeneration by Sirna-027, Am J Ophthalmol, 150, 33, 10.1016/j.ajo.2010.02.006 Nguyen, 2012, Phase 1 dose-escalation study of a siRNA targeting the RTP801 gene in age-related macular degeneration patients, Eye, 26, 1099, 10.1038/eye.2012.106 Nguyen, 2012, Dose-ranging evaluation of intravitreal siRNA PF-04523655 for diabetic macular edema (the DEGAS Study), Invest Ophthalmol Vis Sci, 53, 7666, 10.1167/iovs.12-9961 Kleinman, 2008, Sequence- and target-independent angiogenesis suppression by siRNA via TLR3, Nature, 452, 591, 10.1038/nature06765 Meuli, 2001, Efficient gene expression in skin wound sites following local plasmid injection, J Invest Dermatol, 116, 131, 10.1046/j.1523-1747.2001.00139.x Hengge, 1995, Cytokine gene-expression in epidermis with biological effects following injection of naked DNA, Nat Genet, 10, 161, 10.1038/ng0695-161 Sun, 1997, Transfection with aFGF cDNA improves wound healing, J Invest Dermatol, 108, 313, 10.1111/1523-1747.ep12286471 Sawamura, 2002, The majority of keratinocytes incorporate intradermally injected plasmid DNA regardless of size but only a small proportion of cells can express the gene product, J Invest Dermatol, 118, 967, 10.1046/j.1523-1747.2002.01756.x Ciernik, 1996, Puncture-mediated gene transfer to the skin, Hum Gene Ther, 7, 893, 10.1089/hum.1996.7.8-893 Eriksson, 1998, In vivo gene transfer to skin and wound by microseeding, J Surg Res, 78, 85, 10.1006/jsre.1998.5325 Germershaus, 2014, Application of natural and semi-synthetic polymers for the delivery of sensitive drugs, Int Mater Rev, 60, 101, 10.1179/1743280414Y.0000000045 Cohen-Sacks, 2004, Delivery and expression of pDNA embedded in collagen matrices, J Control Release, 95, 309, 10.1016/j.jconrel.2003.11.001 Capito, 2007, Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering, Gene Ther, 14, 721, 10.1038/sj.gt.3302918 Wang, 2010, Localized SDF-1alpha gene release mediated by collagen substrate induces CD117+ stem cells homing, J Cell Mol Med, 14, 392, 10.1111/j.1582-4934.2008.00624.x des Rieux, 2009, Fibrin hydrogels for non-viral vector delivery in vitro, J Control Release, 136, 148, 10.1016/j.jconrel.2009.02.004 Lei, 2011, Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery, J Control Release, 153, 255, 10.1016/j.jconrel.2011.01.028 Kong, 2008, Design of biodegradable hydrogel for the local and sustained delivery of angiogenic plasmid DNA, Pharm Res, 25, 1230, 10.1007/s11095-007-9526-7 Krebs, 2010, Calcium alginate phosphate-DNA nanoparticle gene delivery from hydrogels induces in vivo osteogenesis, J Biomed Mater Res Part A, 92A, 1131 Han, 2011, Chitosan hydrogel for localized gene silencing, Cancer Biol Ther, 11, 839, 10.4161/cbt.11.9.15185 Segovia, 2014, Hydrogel doped with nanoparticles for local sustained release of siRNA in breast cancer, Adv Healthc Mater Shepard, 2011, Gene therapy vectors with enhanced transfection based on hydrogels modified with affinity peptides, Biomaterials, 32, 5092, 10.1016/j.biomaterials.2011.03.083 Li, 2003, Controlled gene delivery system based on thermosensitive biodegradable hydrogel, Pharm Res, 20, 884, 10.1023/A:1023887203111 Nguyen, 2013, Functionalized, biodegradable hydrogels for control over sustained and localized siRNA delivery to incorporated and surrounding cells, Acta Biomater, 9, 4487, 10.1016/j.actbio.2012.08.012 Lei, 2010, Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds, Biomaterials, 31, 9106, 10.1016/j.biomaterials.2010.08.016 Skiles, 2014 Takahashi, 2010, Device-based local delivery of siRNA against mammalian target of rapamycin (mTOR) in a murine subcutaneous implant model to inhibit fibrous encapsulation, J Control Release, 147, 400, 10.1016/j.jconrel.2010.08.019 Manaka, 2011, Local delivery of siRNA using a biodegradable polymer application to enhance BMP-induced bone formation, Biomaterials, 32, 9642, 10.1016/j.biomaterials.2011.08.026 Kim, 2012, Injectable polyplex hydrogel for localized and long-term delivery of siRNA, ACS Nano, 6, 5757, 10.1021/nn300842a Li, 2012, Supramolecular anchoring of DNA polyplexes in cyclodextrin-based polypseudorotaxane hydrogels for sustained gene delivery, Biomacromolecules, 13, 3162, 10.1021/bm300936x Kim, 2013, An injectable cell penetrable nano-polyplex hydrogel for localized siRNA delivery, Biomaterials, 34, 4493, 10.1016/j.biomaterials.2013.02.050 Nguyen, 2014, Sustained localized presentation of RNA interfering molecules from in situ forming hydrogels to guide stem cell osteogenic differentiation, Biomaterials, 35, 6278, 10.1016/j.biomaterials.2014.04.048 Kwon, 2009, Enhanced angiogenesis mediated by vascular endothelial growth factor plasmid-loaded thermo-responsive amphiphilic polymer in a rat myocardial infarction model, J Control Release, 138, 168, 10.1016/j.jconrel.2009.05.023 Lei, 2010, Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds, Microsc Res Tech, 73, 910, 10.1002/jemt.20840 Orsi, 2010, Design of novel 3D gene activated PEG scaffolds with ordered pore structure, J Mater Sci Mater Med, 21, 1013, 10.1007/s10856-009-3972-1 Gojgini, 2011, Utilizing cell-matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels, Mol Pharm, 8, 1582, 10.1021/mp200171d Shepard, 2010, Balancing cell migration with matrix degradation enhances gene delivery to cells cultured three-dimensionally within hydrogels, J Control Release, 146, 128, 10.1016/j.jconrel.2010.04.032 Keeney, 2013, Modulating polymer chemistry to enhance non-viral gene delivery inside hydrogels with tunable matrix stiffness, Biomaterials, 34, 9657, 10.1016/j.biomaterials.2013.08.050 You, 2010, Bioresponsive matrices in drug delivery, J Biol Eng, 4, 15, 10.1186/1754-1611-4-15 Nagase, 2006, Structure and function of matrix metalloproteinases and TIMPs, Cardiovasc Res, 69, 562, 10.1016/j.cardiores.2005.12.002 Lutolf, 2003, Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics, Proc Natl Acad Sci U S A, 100, 5413, 10.1073/pnas.0737381100 Lee, 2007, Poly(ethylene glycol) hydrogels conjugated with a collagenase-sensitive fluorogenic substrate to visualize collagenase activity during three-dimensional cell migration, Biomaterials, 28, 3163, 10.1016/j.biomaterials.2007.03.004 Raeber, 2005, Molecularly engineered PEG hydrogels: a novel model system for proteolytically mediated cell migration, Biophys J, 89, 1374, 10.1529/biophysj.104.050682 Purcell, 2014, Injectable and bioresponsive hydrogels for on-demand matrix metalloproteinase inhibition, Nat Mater, 13, 653, 10.1038/nmat3922 Lei, 2009, DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells, Biomaterials, 30, 254, 10.1016/j.biomaterials.2008.09.027 Guelcher, 2008, Biodegradable polyurethanes: synthesis and applications in regenerative medicine, Tissue Eng Part B Rev, 14, 3, 10.1089/teb.2007.0133 Nelson, 2011, Controlled release of IGF-1 and HGF from a biodegradable polyurethane scaffold, Pharm Res, 28, 1282, 10.1007/s11095-011-0391-z Li, 2009, The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora, Biomaterials, 30, 6768, 10.1016/j.biomaterials.2009.08.038 Li, 2009, The effect of the local delivery of platelet-derived growth factor from reactive two-component polyurethane scaffolds on the healing in rat skin excisional wounds, Biomaterials, 30, 3486, 10.1016/j.biomaterials.2009.03.008 Li, 2010, Sustained release of vancomycin from polyurethane scaffolds inhibits infection of bone wounds in a rat femoral segmental defect model, J Control Release, 145, 221, 10.1016/j.jconrel.2010.04.002 Nelson, 2012, Sustained local delivery of siRNA from an injectable scaffold, Biomaterials, 33, 1154, 10.1016/j.biomaterials.2011.10.033 Nelson, 2014, Tunable delivery of siRNA from a biodegradable scaffold to promote angiogenesis in vivo, Adv Mater, 26, 607, 10.1002/adma.201303520 Rives, 2009, Layered PLG scaffolds for in vivo plasmid delivery, Biomaterials, 30, 394, 10.1016/j.biomaterials.2008.09.013 Andersen, 2013, Spatially controlled delivery of siRNAs to stem cells in implants generated by multi-component additive manufacturing, Adv Funct Mater, 23, 5599, 10.1002/adfm.201300832 Meinel, 2012, Electrospun matrices for localized drug delivery: current technologies and selected biomedical applications, Eur J Pharm Biopharm, 81, 1, 10.1016/j.ejpb.2012.01.016 Luu, 2003, Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers, J Control Release, 89, 341, 10.1016/S0168-3659(03)00097-X Liang, 2005, In vitro non-viral gene delivery with nanofibrous scaffolds, Nucleic Acids Res, 33 Nie, 2007, Fabrication and characterization of PLGA/HAp scaffolds for delivery of BMP-2 plasmid composite DNA, J Control Release, 120, 111, 10.1016/j.jconrel.2007.03.018 Saraf, 2010, Regulated non-viral gene delivery from coaxial electrospun fiber mesh scaffolds, J Control Release, 143, 95, 10.1016/j.jconrel.2009.12.009 Sakai, 2009, Surface immobilization of poly(ethyleneimine) and plasmid DNA on electrospun poly(L-lactic acid) fibrous mats using a layer-by-layer approach for gene delivery, J Biomed Mater Res Part A, 88A, 281, 10.1002/jbm.a.31870 Li, 2014, Silk fibroin layer-by-layer microcapsules for localized gene delivery, Biomaterials, 35, 7929, 10.1016/j.biomaterials.2014.05.062 Kim, 2010, MMPs-responsive release of DNA from electrospun nanofibrous matrix for local gene therapy: in vitro and in vivo evaluation, J Control Release, 145, 264, 10.1016/j.jconrel.2010.03.006 Blocker, 2011, Surface immobilization of plasmid DNA with a cell-responsive tether for substrate-mediated gene delivery, Langmuir, 27, 2739, 10.1021/la104313z Kim, 2013, In vitro and in vivo epidermal growth factor gene therapy for diabetic ulcers with electrospun fibrous meshes, Acta Biomater, 9, 7371, 10.1016/j.actbio.2013.03.018 Kim, 2013, Matrix metalloproteinase-inspired suicidal treatments of diabetic ulcers with siRNA-decorated nanofibrous meshes, Gene Ther, 20, 378, 10.1038/gt.2012.49 Chen, 2013, Promoted regeneration of mature blood vessels by electrospun fibers with loaded multiple pDNA-calcium phosphate nanoparticles, Eur J Pharm Biopharm, 85, 699, 10.1016/j.ejpb.2013.07.009 He, 2012, Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vessels, Acta Biomater, 8, 2659, 10.1016/j.actbio.2012.03.044 Sokolova, 2006, Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles, Biomaterials, 27, 3147, 10.1016/j.biomaterials.2005.12.030 Miller, 2002, Sonoporation: mechanical DNA delivery by ultrasonic cavitation, Somat Cell Mol Genet, 27, 115, 10.1023/A:1022983907223 Bao, 1997, Transfection of a reporter plasmid into cultured cells by sonoporation in vitro, Ultrasound Med Biol, 23, 953, 10.1016/S0301-5629(97)00025-2 Duvshani-Eshet, 2005, Therapeutic ultrasound optimization for gene delivery: a key factor achieving nuclear DNA localization, J Control Release, 108, 513, 10.1016/j.jconrel.2005.08.025 Deshpande, 2007, Synergistic effect of ultrasound and PEI on DNA transfection in vitro, J Control Release, 118, 126, 10.1016/j.jconrel.2006.12.010 Lentacker, 2009, Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes, Mol Pharm, 6, 457, 10.1021/mp800154s Otani, 2009, Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasound and microbubbles, J Control Release, 133, 146, 10.1016/j.jconrel.2008.09.088 Vandenbroucke, 2008, Ultrasound assisted siRNA delivery using PEG-siPlex loaded microbubbles, J Control Release, 126, 265, 10.1016/j.jconrel.2007.12.001 Florinas, 2014, Ultrasound-assisted siRNA delivery via arginine-grafted bioreducible polymer and microbubbles targeting VEGF for ovarian cancer treatment, J Control Release, 183, 1, 10.1016/j.jconrel.2014.03.025 Pislaru, 2003, Optimization of ultrasound-mediated gene transfer: comparison of contrast agents and ultrasound modalities, Eur Heart J, 24, 1690, 10.1016/S0195-668X(03)00469-X Kowalczuk, 2011, In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles, Ultrasound Med Biol, 37, 1814, 10.1016/j.ultrasmedbio.2011.07.010 Ziadloo, 2013, Pulsed focused ultrasound exposures enhance locally administered gene therapy in a murine solid tumor model, J Acoust Soc Am, 133, 1827, 10.1121/1.4789390 Geng, 2010, Flow-through electroporation based on constant voltage for large-volume transfection of cells, J Control Release, 144, 91, 10.1016/j.jconrel.2010.01.030 Neumann, 1982, Gene-transfer into mouse lyoma cells by electroporation in high electric-fields, EMBO J, 1, 841, 10.1002/j.1460-2075.1982.tb01257.x Bodles-Brakhop, 2009, Electroporation for the delivery of dna-based vaccines and immunotherapeutics: current clinical developments, Mol Ther, 17, 585, 10.1038/mt.2009.5 Gothelf, 2012, What you always needed to know about electroporation based DNA vaccines, Hum Vaccin Immunother, 8, 1694, 10.4161/hv.22062 Wells, 2004, Gene therapy progress and prospects: electroporation and other physical methods, Gene Ther, 11, 1363, 10.1038/sj.gt.3302337 Paganin-Gioanni, 2011, Direct visualization at the single-cell level of siRNA electrotransfer into cancer cells, Proc Natl Acad Sci U S A, 108, 10443, 10.1073/pnas.1103519108 Broderick, 2012, Optimized in vivo transfer of small interfering rna targeting dermal tissue using in vivo surface electroporation, Mol Ther Nucleic Acids, 1, 10.1038/mtna.2012.1 Lee, 2011, A high-capacity, hybrid electro-microneedle for in-situ cutaneous gene transfer, Biomaterials, 32, 7705, 10.1016/j.biomaterials.2011.06.058