Olivier Devuyst1, Ruti Rott1, Jean-François Denef2, Jean Crabbé3, Uri Katz1
1Department of Biology, Technion Institute, 32000 Haifa, Israel
2Department of Histology, UCI Medical School, 10 avenue Hippocrate, B-1200 Brussels, Belgium
3Department of Physiology, UCI Medical School, 10 avenue Hippocrate, B-1200 Brussels, Belgium
Tóm tắt
Summary—Based on immunoblotting procedure, the isolated epithelium of amphibian skin was found to contain a 180 kDa protein which cross‐reacts with a polyclonal antiserum raised against human erythrocyte Band 3. Immunoperoxidase and immunofluorescence staining techniques indicated that the Band 3‐related protein was localized in the mitochondria‐rich cells (MRC) of this epithelium, with characteristic apical labelling pattern. Our findings show that the putative apical anion exchanger of the MRC is immunologically related to the band 3 multigenic family, which catalyzes Cl‐HCO3− transmembranous exchange. It thus suggests a molecular basis for the role played by these cells in the transepithelial Cl pathway and acid‐base regulation.
