Lipid metabolism in tumour bearing mice treated withAeromonas L-asparaginase
Tóm tắt
The anticancerous drug isolated in our laboratory from estuarineAeromonas was characterised and is found to be an enzyme, L-asparaginase. The antileukaemic effect of this drug was studied in mice by inducing leukaemia with Ehrlich ascites cell lines. It was compared with commercially available drug, Leunase, isolated fromE. coli. The lipid profiles in mice during leukaemia and under treatment was studied. The decreased levels of cholesterol and increased levels of triglycerides and phospholipids in serum, liver and kidney were observed in tumour bearing mice. Significant changes in the above values were observed with enzyme therapy. It could bring some of the values to near normal level. L-asparaginase fromAeromonas was found to be more effective.
Tài liệu tham khảo
Koerholz, D., Brueck, M., Nuernberger, W., Juergens, H., Goebel, U. and Wahn, V. (1989) Chemical and immunological characteristics of four different L-asparaginase preparations. Eur. J. Haematol. 42, 417–424.
Raha, S.K., Dey, S.K., Roy, S.K., Chaudhuri, S. and Chakraborty, S.L. (1990) Antitumour activity of L-asparaginase from cylindrocarpon obtusisporm Mβ-10 and its effect on immune system. Biochem. International 21, 1001–1012.
Benny, P.J. and Kurup, G.M. (1991) L-asparaginase activity in bacteria from estuarine sediments and mollusc. Ind. J. Mar. Sci. 20, 36–39.
Benny, P.J. (1993) Biological property of L-asparaginase, Ph.D. thesis, Mahatma Gandhi University, Kottayam, Kerala, India.
Kritchevsky, S.B., Wilkosky, T.E., Morris, D.L., Truong, K.V. and Tyrolev, H.A. (1991) Changes in plasma lipid and lipoprotein, cholesterol and weight prior to the diagnosis of cancer. Cancer Res. (USA) 51, 3198–3203.
William Lee, M.F., Mary, A.W. and Bruce, M.A. (1982) Neutral glycosphingolipids of human acture leukaemias. J. Biol. Chem. 257, 10090–10095.
Jokinen, M., Gahmberg, C.G. and Anderson, L.C. (1979) Biosynthesis of the major human red cell sialoglycoprotein, glycoporin A, in a continuous cell lines. Nature 279, 604–607.
Whitecar, J.P. Jr., Gerald, P.B., Stratton, H. Jr. and Naguib, A.S. (1970) Effect of L-asparaginase on carbohydrate metabolism. Metabolism 19, 581–586.
Vellenga, E., Kluft, C., Mulder, N.H., Wijnards, G. and Nieweg, H.O. (1984) The influence of L-asparaginase therapy on the fibrinolytic system. Br. J. Hematol. 57, 247–254.
Whitecar, J.P., Bodey, G.P., Horris, J.E. and Friereich, E.J. (1970) L-asparaginase. New Engl. J. Med. 282, 732–734.
Haskal, C.M., Canellos, G.P., Laventhal, B.G., Cabone, P.P., Block, J.B., Serpick, A.A. and Salawry, O.S. (1969) L-asparaginase-therapetic and toxic effects in patients with neoplastic disease. New Engl. J. Med. 281, 1028–1034.
Yoon, K.M., Chung, H.S. and Lee, K.S. (1985) Effect of L-asparaginase on the hepatocytes of mice. Hanyang Uidae Haksulchi (Korea) 5, 21–36.
Abell, L.L., Levy, B.B., Brodie, B.B. and Kendell, F.E. (1952) A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J. Biol. Chem. 195, 357.
Kurup, G.M., Leelamma, S. and Kurup, P.A. (1982) Effect of lysine and methionine on lipid metabolism in rats. Ind. J. Biochem. Biophys. 19, 347–351.
Kurup, G.M. (1984) Role of protein and amino acids on the metabolism of lipids, glycoaminoglycans and glycoproteins in rats fed normal and atherogenic diet. Ph.D. Thesis, University of Kerala, India.
John, K.C., Jay, D.L. and Bruce, M.A. (1981) Chemical characterisation of neutral glycolipids in the human myeloid leukaemias. J. Lipid Res. 22, 1079–1083.