Lipid biosignature of breast cancer tissues by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Springer Science and Business Media LLC - Tập 182 - Trang 9-19 - 2020
Catarina L. Silva1, Rosa Perestrelo1, Ivo Sousa-Ferreira2, Filipa Capelinha3, José S. Câmara1,4, Marijana Petković1
1CQM - Centro de Química da Madeira, Universidade da Madeira, Funchal, Portugal
2Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal
3Serviço de Anatomia Patológica, SESARAM, EPE. Hospital Dr. Nélio Mendonça, Funchal, Portugal
4Faculdade de Ciências Exactas e Engenharia da, Universidade da Madeira, Funchal, Portugal

Tóm tắt

One of the hallmarks of cancer cells is the demand of supply for the synthesis of new membranes involved in cell proliferation and lipids have an important role in cellular structure, signaling pathways and progression of cancer. In this sense, lipid studies have become an essential tool allowing the establishment of signatures associated with breast cancer (BC). In this regard, some metabolic processes including proteins, nucleic acids and lipid synthesis are enhanced as part of cancer-associated metabolic reprogramming, as a requirement for cell growth and proliferation. Pairwise samples of breast active carcinoma (BAC) and breast cancer-free tissues were collected from n = 28 patients and analyzed by MALDI-TOF MS. Major lipid species are identified in the MALDI-TOF mass spectra, with certain phosphatidylinositols (PIs) detectable only in BAC. Statistical analysis revealed significant differences (p < 0.05) between ratios lysophosphatidylcholine (LPC) 16:0/phosphatidylcholine (PC) 16:0_18:2 between AC and CF groups as well as for BC stages II and III. The ratio PC 16:0_18:2/PC16:0_18:1 was statistically different between AC and CF groups. The one-way ANOVA revealed that there are no statistical differences among BC stages (I, II and III) within AC group. Comparing BC stages, the significance impact increased (p < 0.05) with stage. The obtained data revealed MALDI-TOF MS as a powerful tool to explore lipid signatures and the enzyme activity associated with BC and possibly establish novel disease markers.

Tài liệu tham khảo

Bray F, Ferlay J, Soerjomataram I et al (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 68:394–424 Koek MM, Jellema RH, van der Greef J et al (2011) Quantitative metabolomics based on gas chromatography mass spectrometry: status and perspectives. Metabolomics 7:307–328 Jelonek K, Ros M, Pietrowska M, Widlak P (2013) Cancer biomarkers and mass spectrometry-based analyses of phospholipids in body fluids. Clin Lipidol 8:137–150 Cho YT, Su H, Chiang YY et al (2017) Fine needle aspiration combined with matrix-assisted laser desorption ionization time-of-flight/mass spectrometry to characterize lipid biomarkers for diagnosing accuracy of breast cancer. Clin Breast Cancer 17:373–381.e1 Cho YT, Su H, Huang TL et al (2013) Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry for clinical diagnosis. Clin Chim Acta 415:266–275 Islam SR, Manna SK (2019) Lipidomic analysis of cancer cell and tumor tissues. Methods Mol Biol 1928:175–204 Long J, Zhang C-J, Zhu N et al (2018) Lipid metabolism and carcinogenesis, cancer development. Am J Cancer Res 8:778–791 Fahy E, Cotter D, Sud M, Subramaniam S (2011) Lipid classification, structures and tools. Biochim Biophys Acta 1811:637–647 Kim IC, Lee JH, Bang G et al (2013) Lipid profiles for HER2-positive breast cancer. Anticancer Res 33:2467–2472 Fuchs B, Schiller J (2008) MALDI-TOF MS analysis of lipids from cells, tissues and body fluids. Subcell Biochem 49:541–565 Du Y, Wang Q, Zhang X et al (2017) Lysophosphatidylcholine acyltransferase 1 upregulation and concomitant phospholipid alterations in clear cell renal cell carcinoma. J Exp Clin Cancer Res 36:66 Abdelzaher E, Mostafa MF (2015) Lysophosphatidylcholine acyltransferase 1 (LPCAT1) upregulation in breast carcinoma contributes to tumor progression and predicts early tumor recurrence. Tumor Biol 36:5473–5483 Uehara T, Kikuchi H, Miyazaki S et al (2016) Overexpression of lysophosphatidylcholine acyltransferase 1 and concomitant lipid alterations in gastric cancer. Ann Surg Oncol 23:206–213 Chen H-Z, Qu Y-H, Diao C-Y et al (2016) Expression of phospholipase A2 in breast cancer tissues and its significance. Int J Clin Exp Pathol 9(11):11820–11825 Yamashita SI, Yamashita JI, Ogawa M (1994) Overexpression of group II phospholipase A2 in human breast cancer tissues is closely associated with their malignant potency. Br J Cancer 69:1166–1170 Kang HS, Lee SC, Park YS et al (2011) Protein and lipid MALDI profiles classify breast cancers according to the intrinsic subtype. BMC Cancer 11:465 Kim KJ, Kim HJ, Park HG et al (2016) A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells. Sci Rep 6:1–7 Phillips L, Gill AJ, Baxter RC (2019) Novel prognostic markers in triple-negative breast cancer discovered by MALDI-mass spectrometry imaging. Front Oncol 9:379 Schiller J, Süß R, Arnhold J et al (2004) Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry in lipid and phospholipid research. Prog Lipid Res 43:449–488 Leopold J, Popkova Y, Engel K, Schiller J (2018) Recent developments of useful MALDI matrices for the mass spectrometric characterization of lipids. Biomolecules 8:173 Fuchs B, Bischoff A, Sü R et al (2009) Phosphatidylcholines and -ethanolamines can be easily mistaken in phospholipid mixtures: a negative ion MALDI-TOF MS study with 9-aminoacridine as matrix and egg yolk as selected example. Anal Bioanal Chem 395:2479–2487 Sevinsky CJ, Khan F, Kokabee L et al (2018) NDRG1 regulates neutral lipid metabolism in breast cancer cells. Breast Cancer Res 20:55 Law S-H, Chan M-L, Marathe GK et al (2019) An updated review of lysophosphatidylcholine metabolism in human diseases. Int J Mol Sci 20:1149 Hao W, Friedman A (2014) The LDL-HDL profile determines the risk of atherosclerosis: a mathematical model. PLoS ONE 9:e90497 Punnonen K, Hietanen E, Auvinen O, Punnonen R (1989) Phospholipids and fatty acids in breast cancer tissue. J Cancer Res Clin Oncol 115:575–578 Goto T, Terada N, Inoue T et al (2014) The expression profile of phosphatidylinositol in high spatial resolution imaging mass spectrometry as a potential biomarker for prostate cancer. PLoS ONE 9:e90242 Sparvero LJ, Amoscato AA, Dixon CE et al (2012) Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations. Chem Phys Lipids 165:545–562 Harvey DJ (1996) Matrix-assisted laser desorption/ionisation mass spectrometry of oligosaccharides and glycoconjugates. J Chromatogr A 720:429–446 Petković M, Schiller J, Müller M et al (2001) Detection of individual phospholipids in lipid mixtures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: phosphatidylcholine prevents the detection of further species. Anal Biochem 289:202–216 Fuchs B, Süß R, Schiller J (2010) An update of MALDI-TOF mass spectrometry in lipid research. Prog Lipid Res 49:450–475 Petković M, Vocks A, Müller M et al (2005) Comparison of different procedures for the lipid extraction from HL-60 cells: A MALDI-TOF mass spectrometric study. Zeitschrift fur Naturforsch-Sect C J Biosci 60:143–151 Teuber K, Riemer T, Schiller J (2010) Thin-layer chromatography combined with MALDI-TOF-MS and 31P-NMR to study possible selective bindings of phospholipids to silica gel. Anal Bioanal Chem 398:2833–2842 Schiller J, Süß R, Fuchs B et al (2007) The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: Which isomer for what purpose? Eur Biophys J 36:517–527 Schiller J, Süß R, Petković M, Arnold K (2002) Thermal stressing of unsaturated vegetable oils: Effects analysed by MALDI-TOF mass spectrometry,1H and31P NMR spectroscopy. Eur Food Res Technol 215:282–286 Schiller J, Arnhold J, Benard S et al (1999) Lipid analysis by matrix-assisted laser desorption and ionization mass spectrometry: a methodological approach. Anal Biochem 267:46–56 Angelini R, Vortmeier G, Corcelli A, Fuchs B (2014) A fast method for the determination of the PC/LPC ratio in intact serum by MALDI-TOF MS: An easy-to-follow lipid biomarker of inflammation. Chem Phys Lipids 183:169–175 Miletić Vukajlović J, Drakulić D, Pejić S et al (2019) Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson’s disease. Rapid Commun Mass Spectrom 34:8595 Kamčeva T, Radisavljevic̈ M, Vukic̈evic̈ I, et al (2013) Interactions of platinum and ruthenium coordination complexes with pancreatic phospholipase A 2 and phospholipids investigated by MALDI TOF mass spectrometry. Chem Biodivers 10:1972–1986 Petković M, Müller J, Müller M et al (2002) Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for monitoring the digestion of phosphatidylcholine by pancreatic phospholipase A2. Anal Biochem 308:61–70 Kim Y, Shanta SR, Zhou LH, Kim KP (2010) Mass spectrometry based cellular phosphoinositides profiling and phospholipid analysis: A brief review. Exp Mol Med 42:1–11 Margaria JP, Ratto E, Gozzelino L et al (2019) Class II PI3Ks at the intersection between signal transduction and membrane trafficking. Biomolecules 9:104 Dogliotti G, Kullmann L, Dhumale P et al (2017) Membrane-binding and activation of LKB1 by phosphatidic acid is essential for development and tumour suppression. Nat Commun 8:1–2 Müller M, Schiller J, Petković M et al (2001) Limits for the detection of (poly-)phosphoinositides by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chem Phys Lipids 110:151–164 Milne SB, Ivanova PT, DeCamp D et al (2005) A targeted mass spectrometric analysis of phosphatidylinositol phosphate species. J Lipid Res 46:1796–1802