Linear epitope mapping of humoral responses induced by vaccination with recombinant HIV-1 envelope protein gp160

Oxford University Press (OUP) - Tập 19 - Trang 58-65 - 1997
L D Loomis-Price1, M Levi2, P R Burnett3, J E van Hamont4, R A Shafer5, B Wahren2, D L Birx4
1HIV Laboratory, Henry M Jackson Foundation, Rockville, MD 20850, USA, , US
2Swedish Institute for Infectious Disease Control and Karolinska Institute, Stockholm, Sweden, , SE
3US Army Dental Research Detachment, Washington DC 20307, USA, , US
4Walter Reed Army Institute of Research, Washington DC 20307, USA, , US
5Goddard Space Flight Center, National Aeronautics and Space Administration, Greenbelt, MD 20771, USA, , US

Tóm tắt

To enhance utility of the linear epitope mapping (Pepscan) technique for assay of humoral responses linked to vaccination, two modifications were tested. First, peptides were incubated with serum contained in baths rather than individual wells. Second, a rigorous statistical model was developed to determine which peptide/antibody-binding interactions were significant. The modifications increased the ability to detect signal in these experiments by 15- to 45-fold. These two modifications were applied to linear epitope mapping of HIV seropositive volunteers under treatment with recombinant HIV gp160 and also to rabbits immunized with the same product. Changes in fine specificity of response were observed in animal models and human vaccine recipients over the course of an immunization series with this antigen.

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Oxford University Press (OUP)

Tập 19

58-65

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