Lignocellulose degradation by the isolate of Streptomyces griseorubens JSD-1

Springer Science and Business Media LLC - 2014
Haiwei Feng1,2,3, Yujing Sun2, Yuee Zhi1,2,3, Liang Mao1,2,3, Yanqing Luo1,2,3, Xing Wei1,2,3, Pei Zhou1,2
1Bor.S.Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, China
2School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
3Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai, China

Tóm tắt

Streptomyces griseorubens JSD-1 is an isolate that can utilize lignocellulose (straw) as its sole carbon source for growth, and these lignocellulolytic genes involved in this biotransformation are expected to be crucial. However, little is known about the genetic basis related to this process. To further investigate the lignocellulose-degrading mechanisms, genome sequencing was carried out using MiSeq platform. After obtaining its draft genome, the key lignocellulolytic genes such as multicopper oxidase, exo-1, 4-β-glucanase, endo-1,4-β-glucanase, and β-xylosidase were identified and characterized. Multiple sequence alignments were performed to find out the identities of these analyzed proteins to those of their similar species. Signal peptide cleavage sites were predicted by SignalP 4.1 to reflect the cellular localization of their mature proteins. Besides, predicted 3D structures of these proteins were modeled by Phyre2, which showed to be highly identical to the templates in the PDB database. Finally, quantitative real-time PCR revealed that expression levels of all analyzed enzymes were significantly and generally up-regulated during the whole cultivation, indicating that they all contributed to the biodegradation.

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