Leukocyte‐Platelet‐Rich Plasma (L‐PRP) Induces an Abnormal Histophenotype in Craniofacial Bone Repair Associated with Changes in the Immunopositivity of the Hematopoietic Clusters of Differentiation, Osteoproteins, and TGF‐β1

Clinical Implant Dentistry and Related Research - Tập 16 Số 2 - Trang 259-272 - 2014
Allan Fernando Giovanini1, João Ricardo Almeida Grossi1, Carla Castiglia Gonzaga1, João César Zielak1, Isabella Göhringer1, Juliana de Souza Vieira1, Juliane Kuczera2, Marco Antonio de Oliveira Filho3, Tatiana Miranda Deliberador1
1Masters Program in Clinical Dentistry, Positivo University, Curitiba, Brazil
2School of Dentistry, Positivo University, Curitiba, Brazil
3Head and Neck Surgery Service, Evangelic Hospital, Curitiba, Brazil

Tóm tắt

AbstractBackground

Leukocyte‐platelet‐rich plasma (L‐PRP) is considered an important source of growth factors, especially Transforming growth factor β 1 (TGF‐β1), which modulates the proliferation and regulation of mesenchymal cells, and also exerts an influence on the hematopoiesis, osteogenesis, and adipogenesis in bone microenvironment. Thus, the aim of this study was to evaluate the effect of L‐PRP on the calvarial bone repair and compare its results on the presence of TGF‐β1, CD34, CD45, bone morphogenetic protein 2 (BMP2), BMPR1B, and Runx2 proteins detected by immunohistochemistry.

Material and Methods

Four bone defects were created on the calvaria of 23 rabbits. The defects were treated with autograft, L‐PRP alone, and L‐PRP mixed with autograft. The animals were euthanized at 2, 4, and 6 weeks post‐surgery.

Results

Unlike autograft and sham groups, the defects treated with L‐PRP demonstrated significant positivity to TGF‐β1, while the BMP2 was scarce. These results coincided with the lower bone matrix deposited and larger medullary area, which were composed of fibrosis, when treated with only L‐PRP, or intense adiposity on defects filled with L‐PRP mixed with autograft. The fibrosis that occurred was associated with a minor percentage of osteoproteins, intense presence of CD34+ CD45− cells, and significant expression of TGF‐β1 in all time periods analyzed. The adiposity occurred from the major presence of osteoprogenitor BMPR1B + Runx2+ cells simultaneously to BMP2− TGF‐β1+ and CD34+ CD45+/− expressions predominantly on the earlier period.

Conclusion

From this study, it can be concluded that the L‐PRP used alone or mixed to autograft hindered the osteoneogenesis due to suppression of immunoexpression of BMP2, while the immunopositivity of TGF‐β1 was intense. When used alone, the L‐PRP induced a fibrotic condition associated with TGF‐β1 presence and lack of osteoproteins, but when L‐PRP was mixed to autograft, it induced the presence of the osteolineage cells (BMPR1B + Runx2+), but also inhibited the terminal osteoblastic maturation associated with the lack of BMP2 and the presence of TGF‐β1+, a fact that contributed to cellular transdifferentiation into fat cells.

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Tài liệu tham khảo

Aghaloo TL, 2004, Evaluation of platelet‐rich plasma in combination with anorganic bovine bone in the rabbit cranium: a pilot study, Int J Oral Maxillofac Implants, 19, 59

10.1016/j.joms.2003.08.021

10.1016/j.joms.2003.12.003

10.1182/blood-2002-12-3882

10.1074/jbc.M308864200

10.1097/01.PRS.0000138251.07040.51

10.1089/ten.tea.2009.0832

10.1016/j.biomaterials.2009.05.055

10.1089/ten.teb.2008.0062

10.1089/ten.tea.2010.0227

Arnoczky SP, 2011, What is platelet‐rich plasma? Operative techniques in sports, Medicine, 19, 142

10.1016/j.tibtech.2008.11.009

10.1016/S1079-2104(98)90029-4

10.1016/j.bone.2010.02.026

10.1002/jcb.20777

10.1016/j.abb.2008.03.028

10.1210/er.2005-0022

10.1182/blood-2008-02-139824

10.1182/blood.V96.6.2022

Majeti R, 2007, Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood, Cell, 1, 635

10.1126/science.1091903

10.1172/JCI0215916

10.1084/jem.20080072

10.1111/j.1365-2141.1994.tb04972.x

10.1038/87687

10.1247/csf.08038

10.1016/j.cytogfr.2009.10.018

10.1016/j.bone.2010.10.178

10.1242/dev.002709

10.1387/ijdb.052101xn

10.1073/pnas.0604849103

10.1523/JNEUROSCI.4459-09.2010

Ashique AM, 2002, Signalling via type IA and type IB bone morphogenetic protein receptors (BMPR) regulates intramembranous bone formation, chondrogenesis and feather formation in the chicken embryo, Int J Dev Biol, 46, 243

10.1083/jcb.142.1.295

10.1016/j.cmpb.2010.02.002

10.1126/science.1181928

10.1111/j.1600-0501.2005.01215.x

10.1111/j.1600-051X.2006.00938.x

10.1054/ijom.2003.0452

10.1053/joms.2002.34994

10.1034/j.1600-0501.2003.00928.x

10.1902/jop.2005.76.8.1287

10.1016/j.autrev.2010.09.016

10.1016/j.ajpath.2011.08.027

10.1016/S0002-9440(10)62353-X

10.1002/jor.21027

10.1016/j.bone.2009.04.246

10.1242/dev.015933

10.1210/er.2002-0023

10.1359/jbmr.2003.18.10.1842

10.1016/S8756-3282(03)00052-8

10.1182/blood.V81.9.2301.2301

10.1182/blood.V82.7.2069.bloodjournal8272069

10.1590/S0102-86502010000100008

10.1054/ijom.2003.0466

10.1016/0049-3848(80)90317-5

10.1016/0049-3848(93)90118-8

10.2174/187152907780059056

10.1161/01.ATV.10.2.276

10.1016/j.thromres.2005.11.012

10.1359/jbmr.1997.12.8.1189

10.1172/JCI31487

10.1152/ajpgi.2000.279.5.G845

10.1016/j.bbadis.2009.06.004

10.1002/jor.21263

10.1038/nature02041

10.1016/S8756-3282(01)00580-4

10.1038/sj.emboj.7600729

10.1385/ENDO:23:2-3:183

10.1074/jbc.M212259200

10.1016/j.bone.2005.06.020

10.1016/j.bone.2005.10.027

10.1016/j.bone.2008.05.009

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Clinical Implant Dentistry and Related Research

Tập 16 Số 2

259-272

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