Laser fluorescence of dentin caries covered with a novel nano-filled sealant
Tóm tắt
The aim of the present study was to assess the possibility to measure caries-induced laser fluorescence underneath a novel nano-filled fissure sealant. Sixty freshly extracted human teeth with occlusal dentine carious lesions were horizontally divided, exposing the respective lesion. Teeth were randomly assigned to three groups: (I) white fissure sealant with filler particles (Fissurit F, Voco), (II) clear fissure sealant without filler particles (Fissurit, Voco) and (III) novel experimental fissure sealant with nano-filler particles (Voco). Starting with a sealant thickness of 3 mm, laser fluorescence measurements (DIAGNOdent, KaVo) were performed after finishing the sealant surfaces with polishing papers, reducing the material at intervals of 0.5 mm until the sealant was removed completely. Evaluating a thickness of 0.5 mm, both the clear (83 % of the baseline fluorescence after fine grit polishing) and the white sealant (25 %) did not allow to measure baseline fluorescence (p < 0.05) with no fluorescence reduction in the experimental sealant group (p > 0.05). With increasing sealer thickness, fluorescence was influenced even by the experimental material (89 % of the baseline value at 1 mm). However, by using the experimental material, statistically significant higher fluorescence values than those for the other materials under study (p < 0.05) were obtained. Thicker sealant layers and coarse grit polishing caused a decrease of laser fluorescence in all groups (p < 0.05). Employing the experimental nano-filled sealant, laser fluorescence measurements for caries detection can be performed through thicker sealant layers compared to conventional sealant materials. Thus, it might be possible to use this material to assess a caries progression underneath the sealant and administer an appropriate therapy in due time.
Tài liệu tham khảo
Stuebel H (1911) The fluorescence of animal tissues by irradiation with ultraviolet light. Arch Ges Physiol 142:1–14
Bjelkhagen H, Sundström F, Angmar-Månsson B, Ryde H (1982) Early detection of enamel caries by the luminescence excited by visible laser light. Swed Dent J 6:1–7
Sundström F, Fredikson K, Montan S, Hafström-Björkman U, Ström J (1985) Laser-induced fluorescence from sound and carious tooth substance: spectroscopic studies. Swed Dent J 36:315–319
Lussi A, Hibst R, Paulus R (2004) DIAGNOdent: an optical method for caries detection. J Dent Res 83(Spec Iss C):80–83
König K, Flemming G, Hibst R (1998) Laser-induced autofluorescence spectroscopy of dental caries. Cell Mol Biol 44:1293–1300
Banerjee A, Gilmour A, Kidd E, Watson T (2004) Relationship between Streptococcus mutans and the autofluorescence of carious dentin. Am J Dent 17:233–236
Braun A, Graefen O, Frentzen M, Nolden R (2000) Comparative study of conventional caries diagnosis versus laser fluorescence measurement. Dtsch Zahnärztl Z 55:248–251
Lussi A, Megert B, Longbottom C, Reich E, Francescut P (2001) Clinical performance of a laser fluorescence device for detection of occlusal caries lesions. Eur J Oral Sci 109:14–19
Lussi A, Francescut P (2003) Performance of conventional and new methods for the detection of occlusal caries in deciduous teeth. Caries Res 37:2–7
Alwas-Danowska HM, Plasschaert AJ, Suliborski S, Verdonschot EH (2002) Reliability and validity issues of laser fluorescence measurements in occlusal caries diagnosis. J Dent 30:129–134
Pinelli C, Campos Serra M, de Castro Monteiro Loffredo L (2002) Validity and reproducibility of a laser fluorescence system for detecting the activity of white-spot lesions on free smooth surfaces in vivo. Caries Res 36:19–24
Ahovuo-Saloranta A, Hiiri A, Nordblad A, Mäkelä M, Worthington HV (2008) Pit and fissure sealants for preventing dental decay in the permanent teeth of children and adolescents. Cochrane Database Syst Rev 8:CD001830
Donly KJ (2002) Sealants: where we have been; where we are going. Gen Dent 50:438–440
Mejàre I, Lingström P, Petersson LG, Holm AK, Twetman S, Källestal C, Nordenram G, Lagerlöf F, Söder B, Norlund A, Axelsson S, Dahlgren H (2003) Caries preventive effect of fissure sealants: a systematic review. Acta Odontol Scand 61:321–330
Handelman SL, Wasburn F, Wopperer P (1976) Two-year report of sealant effect on bacteria in dental caries. J Am Dent Assoc 93:967–970
Jeronimus DJ, Till MJ, Sveen OB (1975) Reduced viability of microorganisms under dental sealants. J Am Dent Assoc 42:275–280
Kramer PF, Zelante F, Simionato MR (1993) The immediate and long-term effects of invasive and noninvasive pit and fissure sealing techniques on the microflora in occlusal fissures of human teeth. Pediatr Dent 15:108–112
Mertz-Fairhurst EJ, Curtis JW Jr, Ergle JW, Rueggeberg FA, Adair SM (1998) Ultraconservative and cariostatic sealed restorations: results at year 10. J Am Dent Assoc 129:55–66
Feigal RJ (2002) The use of pit and fissure sealants. Pediatr Dent 24:415–422
Weerheijm KL, de Soet JJ, van Amerongen WE, de Graaff J (1992) Sealing of occlusal hidden caries lesions: an alternative for curative treatment? J Dent Child 59:263–268
Krause F, Braun A, Frentzen M, Jepsen S (2008) Effects of composite fissure sealants on IR laser fluorescence measurements. Lasers Med Sci 23:133–139
Kidd EA (2004) How ‘clean’ must a cavity be before restoration? Caries Res 38:305–313
Kidd EA (2010) Clinical threshold for carious tissue removal. Dent Clin N Am 54:541–549
Hotz PR (2003) Must caries be completely removed? Schweiz Monatsschr Zahnmed 113:262–266
Francescut P, Lussi A (2006) Performance of a conventional sealant and a flowable composite on minimally invasive prepared fissures. Oper Dent 31:543–550
Ilie N, Hickel R (2009) Investigations on mechanical behaviour of dental composites. Clin Oral Investig 13:427–438
Terry DA (2004) Direct applications of a nanocomposite resin system: part 1—the evolution of contemporary composite materials. Pract Proced Aesthet Dent 16:417–422
Palaniappan S, Elsen L, Lijnen I, Peumans M, Van Meerbeek B, Lambrechts P (2010) Three-year randomised clinical trial to evaluate the clinical performance, quantitative and qualitative wear patterns of hybrid composite restorations. Clin Oral Investig 14:441–458
Braun A, Krause F, Jepsen S (2005) The influence of the calibration mode of a laser fluorescence device on caries detection. Caries Res 39:144–149
Braun A, Frentzen M, Nolden R (1999) Effects of tooth cleaning on laser fluorescence measurements on the tooth surface. Dtsch Zahnärztl Z 54:195–197
Deery C (2010) Brushing as good as handpiece prophylaxis before placing sealants. Evid Based Dent 11:79–80
Jefferies SR, Boston DW (2010) Conventional polishing techniques versus a nanofilled surface sealer: preliminary findings regarding surface roughness changes and analysis. J Clin Dent 21:20–23
Krämer N, García-Godoy F, Reinelt C, Feilzer AJ, Frankenberger R (2011) Nanohybrid vs. fine hybrid composite in extended class II cavities after six years. Dent Mater 27:455–464