Larvicidal activity of Copaifera sp. (Leguminosae) oleoresin microcapsules against Aedes aegypti (Diptera: Culicidae) larvae
Tóm tắt
Studies have demonstrated the potential of Copaifera sp. oleoresin to control Aedes aegypti proliferation. However, the low water solubility is a factor that limits its applicability. Thus, the micro- or nanoencapsulation could be an alternative to allow its use in larval breeding places. The purpose of this study was to evaluate if achievable lethal concentrations could be obtained from Copaifera sp. oleoresin incorporated into polymers (synthetic or natural) and, mainly, if it can be sustained in the residual activity compared to the pure oil when tested against the A. aegypti larvae. Microcapsules were prepared by the process of emulsification/precipitation using the polymers of cellulose acetate (CA) and poly(ethylene-co-methyl acrylate) (PEMA), yielding four types of microcapsules: MicPEMA1 and MicPEMA2, and MicCA1 and MicCA2. When using only Copaifera sp. oleoresin, the larvicidal activity was observed at concentrations of LC50 = 48 mg/L and LC99 = 149 mg/L. For MicPEMA1, the LC50 and LC99 were 78 and 389 mg/L, respectively. Using MicPEMA2, the LC50 was 120 mg/L and LC99 >500 mg/L. For microcapsules MicCA1 and MicCA2, the LC50 and LC99 were 42, 164, 140, and 398 mg/L, respectively. For a dose of 150 mg/L of pure oleoresin, the residual activity remained above 20% for 10 days, while the dose of 400 mg/L remained above 40% for 21 days. The MicPEMA1 microcapsules showed a loss in residual activity up to the first day; however, it remained in activity above 40% for 17 days. The microcapsules of MicCA1 showed similar LC50 of pure oil with 150 mg/L.
Tài liệu tham khảo
Anjali CH, Khan SS, Goshen KM, Magdassi S, Mukherjee A, Chandrasekaran N (2010) Formulation of water-dispersible nanopermethrin for larvicidal applications. Ecotoxicol Environ Saf 73:1932–1936
Cavalcanti ESB, Morais SM, Lima MAA, Eddi SEWP (2004) Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Mem Inst Oswaldo Cruz 99:541–544
Cheng SS, Chang HT, Lin CY, Chen PS, Huang CG, Chen WJ, Chang ST (2009) Insecticidal activities of leaf and twig essential oils from Clausena excavata against Aedes aegypti and Aedes albopictus larvae. Pest Manag Sci 65:339–343
Conti B, Canale A, Bertoli A, Gozzini F, Pistelli L (2010) Essential oil composition and larvicidal activity of six Mediterranean aromatic plants against the mosquito Aedes albopictus (Diptera: Culicidae). Parasitol Res 6:1455–1461
Dahl C (1988) Control potentials in feeding mechanisms of mosquito larvae. Bull Soc Vector Ecol 13:295–303
Domb AJ, Tabata Y, Kumar MNVR, Farber S (2007) Nanoparticles for pharmaceutical applications. American Scientific, Valencia, 500 pp
Einsenberg JNS, Washburn JO, Schreiber SJ (2000) Generalist feeding behaviors of Aedes sierrensis larvae and their effects on protozoan populations. Ecology 81:921–935
Elek N, Hoffman R, Raviv U, Resh R, Ishaaya I, Magdassi S (2010) Novaluron nanoparticles: formation and potential use in controlling agricultural insect pests. Colloid Surface Physicochem Eng Aspect 372:66–72
Evergetis E, Michaelakis A, Kioulos E, Koliopoulos G, Haroutounian AS (2009) Chemical composition and larvicidal activity of essential oils from six Apiaceae family taxa against the West Nile virus vector Culex pipiens. Parasitol Res 105(1):117–124
Flores FC, Ribeiro RF, Ourique AF, Rolim CMB, Silva CB (2011) Nanostructured systems containing an essential oil: protection against volatilization. Quím Nova 34(6):968–972
Forattinni OP (2002) Culicidologia medica. Editora da Universidade de São Paulo, São Paulo, 860
Geris R, Silva IG, Silva HH, Barison A, Rodrigues-Filho E, Ferreira AG (2008) Diterpenoids from Copaifera reticulata Ducke with larvicidal activity against Aedes aegypti (L.) (Diptera, Culicidae). Rev Inst Med Trop Sao Paulo 50:25–28
Gonsalves JKMC, Costa AMB, Sousa DP, Cavalcanti SCH, Nunes RS (2009) Microencapsulação do óleo essencial de Citrus sinensis (L) Osbeck pelo método da coacervação simples. Scientia Plena 5:111–102
Hirech K, Payan S, Carnelle G, Brujes L, Legrand J (2003) Microencapsulation of an insecticide by interfacial polymerization. Powder Technol 130:324–330
Jayaseelan C, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Marimuthu S, Bagavan A, Kamaraj C, Zahir AA, Elango G, Velayutham K, Rao KVB, Karthik L, Raveendran S (2011) Efficacy of plant-mediated synthesized silver nanoparticles against hematophagous parasites. Parasitol Res. doi:10.1007/s00436-011-2473-6
Kanis LA, Generoso M, Soldi V (2007) Filmes de poli(etileno-co-metil acrilato)/poli(caprolactona triol): caracterização e propriedades mecânicas. Lat Am J Pharm 26:700–705
Meier MA, Kanis LA, Soldi V (2004) Characterization and drug-permeation profiles of microporous and dense cellulose acetate membranes: influence of plasticizer and pore forming agent. Int J Pharm 278:99–110
Mendonça FAC, Da Silva KFS, Dos Santos KK, Júnior KALR, Sant’Ana AEG (2005) Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti. Fitoterapia 76:629–636
Merritt RW, Dadd RH, Walker ED (1992) Feeding behavior, natural food, and nutritional relationships of larva mosquitoes. Annu Rev Entomol 37:349–376
Paula HCB, Sombra FM, Abreu FOMS, Paul RCM (2010) Lippia sidoides essential oil encapsulation by angico gum/chitosan nanoparticles. J Braz Chem Soc 21:2359–2366
Pavela R (2008) Larvicidal effects of various Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae). Parasitol Res 102:555–559
Prophiro JS, Silva MN, Kanis LA, Silva BM, Duque-Luna JE, Silva OS (2011) Evaluation of time toxicity, residual effect, and growth-inhibiting property of Carapa guianensis and Copaifera sp. in Aedes aegypti. Parasitol Res. doi:10.1007/s00436-011-2547-5
Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008) Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 102:867–873
Santhoshkumar T, Rahuman AA, Rajakumar G, Marimuthu S, Bagavan A et al (2011) Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res 108:693–702
Shanmugasundaram R, Jeyalakshmi T, Dutt MS, Murthy PB (2008) Larvicidal activity of neem and karanja oil cakes against mosquito vectors, Culex quinquefasciatus (say), Aedes aegypti (L.) and Anopheles stephensi (L.). J Environ Biol 29:43–45
Silva IG, Zanon VOM, Silva HHG (2003) Larvicidal activity of Copaifera reticulata Ducke oil-resin against Culex quinquefasciatus Say (Diptera: Culicidae). Neotrop Entomol 32:729–732
Silva OS, Prophiro JS, Rossi JCN, Kanis LA, Romão PRT, Blazius RD (2006) Larvicidal effect of andiroba oil Carapa guianensis (Meliaceae) against Aedes aegypti (Diptera: Culicidae). J Am Mosq Control Assoc 22:699–701
Silva HHG, Geris R, Rodrigues E, Rocha C, Silva IG (2007) Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae). Rev Soc Bras Med Trop 40:264–267
Silva WJ, Dória GA, Maia RT, Nunes RS, Carvalho GA, Blank AF, Alves PB, Marçal RM, Cavalcanti SC (2008) Effects of essential oils on Aedes aegypti larvae: alternatives to environmentally safe insecticides. Bioresour Technol 99:3251–3255
Surassmo S, Min SG, Bejrapha P, Choi MJ (2010) Effects of surfactants on the physical properties of capsicum oleoresin-loaded nanocapsules formulated through the emulsion–diffusion method. Food Res Int 43:8–17
Warikoo R, Kumar NS (2006) Larvicidal efficacy and biological stability of a botanical natural product, zedoary oil-impregnated sand granules, against Aedes aegypti (Diptera, Culicidae). Parasitol Res 100:729–737
World Health Organization (1981a) Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO Geneva 81.807
World Health Organization (1981b). Criteria and meaning of tests for determining the susceptibility or resistance of insects to insecticides. WHO Geneva 81.806