Large-scale Expression and Purification of a G-protein-coupled Receptor for Structure Determination – An Overview

Reinhard Grisshammer1, Jim F. White1, Loc Trinh2, Joseph Shiloach2
1Laboratory of Molecular Biology of the National Institute of Diabetes and Digestive and Kidney Diseases, Department of Health and Human Services, National Institutes of Health, Bethesda, USA
2Biotechnology Unit of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, National Institutes of Health, Bethesda, USA

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Reprinted by permission of Federation of the European Biochemical Societies from ‘Automated large-scale purification of a G-protein-coupled receptor for neurotensin’, by White et al., Febs Lett. 564, 289–293, 2004