Lactucin & Lactucopicrin ameliorates FFA-induced steatosis in HepG2 cells via modulating lipid metabolism

Loomba, 2021, Mechanisms and disease consequences of nonalcoholic fatty liver disease, Cell, 184, 2537, 10.1016/j.cell.2021.04.015 2020, 323 Adalaiti, 2022, Anti-liver fibrosis activity in vitro of Cichorium glandulosum seed and root compatibility, Zhong Nan Yao Xue, 9, 1077 Huang, 2014, Protective effect of Cichorium glandulosum seeds from ultraviolet B-induced damage in rat liver mitochondria, Food Funct, 5, 869, 10.1039/c3fo60466g Ding, 2014, Lipid modulatory activities of Cichorium glandulosum Boiss et Huet are mediated by multiple components within hepatocytes, Sci Rep, 4, 4715, 10.1038/srep04715 Yang, 2012, Hepatoprotective activities of a sesquiterpene-rich fraction from the aerial part of Cichorium glandulosum, Chin Med, 7, 21, 10.1186/1749-8546-7-21 Meng, 2021, Recent pharmacological advances in the repurposing of artemisinin drugs, Med Res Rev, 41, 3156, 10.1002/med.21837 Ru, 2014, TCMSP: a database of systems pharmacology for drug discovery from herbal medicines, J Cheminform, 6, 13 Tian, 2015, The application of in silico drug-likeness predictions in pharmaceutical research, Adv Drug Deliv Rev, 86, 2, 10.1016/j.addr.2015.01.009 Wang, 2020, A potential nutraceutical candidate lactucin inhibits adipogenesis through downregulation of JAK2/STAT3 signaling pathway-mediated mitotic clonal expansion, Cells, 9, 331, 10.3390/cells9020331 Rasmussen, 2014, Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.), Food Chem, 146, 255, 10.1016/j.foodchem.2013.09.068 He, 2021, Lactucopicrin inhibits cytoplasmic dynein-mediated NF-κB activation in inflammated macrophages and alleviates atherogenesis in apolipoprotein E-deficient mice, Mol Nutr Food Res, 65, 10.1002/mnfr.202000989 Kwok, 2020, Whole-transcriptome sequencing (RNA-seq) study of the ZFL zebrafish liver cell line after acute exposure to Cd 2+ ions, Aquat Toxicol, 228, 105628, 10.1016/j.aquatox.2020.105628 Liu, 2016, RNA sequencing reveals retinal transcriptome changes in STZ-induced diabetic rats, Mol Med Rep, 13, 2101, 10.3892/mmr.2016.4793 Liu, 2021, An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury, J Ethnopharmacol, 264, 113364, 10.1016/j.jep.2020.113364 Chen, 2021, Global gene expression analysis using RNA-seq reveals the new roles of Panax notoginseng Saponins in ischemic cardiomyocytes, J Ethnopharmacol, 268, 113639, 10.1016/j.jep.2020.113639 Yang, 2022, A triple combination strategy of UHPLC-MS, hypolipidemic activity and transcriptome sequencing to unveil the hypolipidemic mechanism of Nelumbo nucifera alkaloids, J Ethnopharmacol, 282, 114608, 10.1016/j.jep.2021.114608 Parkhomchuk, 2009, Transcriptome analysis by strand-specific sequencing of complementary DNA, Nucleic Acids Res, 37, e123, 10.1093/nar/gkp596 Li, 2015, Quality control of RNA-seq experiments, Methods Mol Biol, 1269, 137, 10.1007/978-1-4939-2291-8_8 Mao, 2005, Automated genome annotation and pathway identification using the KEGG orthology (KO) as a controlled vocabulary, Bioinformatics, 21, 3787, 10.1093/bioinformatics/bti430 Young, 2010, Gene ontology analysis for RNA-seq: accounting for selection bias, Genome Biol, 11, R14, 10.1186/gb-2010-11-2-r14 Cicinnati, 2008, Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR, BMC Cancer, 8, 350, 10.1186/1471-2407-8-350 Zhang, 2021, A network pharmacology based approach for predicting active ingredients and potential mechanism of Lianhuaqingwen capsule in treating COVID-19, Int J Med Sci, 18, 1866, 10.7150/ijms.53685 Day, 1998, Steatohepatitis: a tale of two “hits”, Gastroenterology, 114, 842, 10.1016/S0016-5085(98)70599-2 Donato, 2008, Cell lines: a tool for in vitro drug metabolism studies, Curr Drug Metab, 9, 1, 10.2174/138920008783331086 Chen, 2020, Selection of suitable reference genes for qPCR gene expression analysis of HepG2 and L02 in four different liver cell injured models, BioMed Res Int, 2020, 8926120 Lee, 2019, Lycopus lucidus Turcz. ex Benth. Attenuates free fatty acid-induced steatosis in HepG2 cells and non-alcoholic fatty liver disease in high-fat diet-induced obese mice, Phytomedicine, 55, 14, 10.1016/j.phymed.2018.07.008 Hetherington, 2016, Differential lipotoxic effects of palmitate and oleate in activated human hepatic stellate cells and epithelial hepatoma cells, Cell Physiol Biochem, 39, 1648, 10.1159/000447866 Wang, 2022, Overexpression of NAG-1/GDF15 prevents hepatic steatosis through inhibiting oxidative stress-mediated dsDNA release and AIM2 inflammasome activation, Redox Biol, 52, 102322, 10.1016/j.redox.2022.102322 Brown, 2021, Emerging and established therapeutic approaches for nonalcoholic fatty liver disease, Clin Ther, 43, 1476, 10.1016/j.clinthera.2021.07.013 Zhao, 2020, An AMPK-caspase-6 axis controls liver damage in nonalcoholic steatohepatitis, Science, 367, 652, 10.1126/science.aay0542 Sun, 2021, Regulation on citrate influx and metabolism through inhibiting SLC13A5 and ACLY: a novel mechanism mediating the therapeutic effects of curcumin on NAFLD, J Agric Food Chem, 69, 8714, 10.1021/acs.jafc.1c03105 Zhao, 2021, Bicyclol ameliorates nonalcoholic fatty liver disease in mice via inhibiting MAPKs and NF-KappaB signaling pathways, Biomed Pharmacother, 141, 111874, 10.1016/j.biopha.2021.111874 Kakehashi, 2017, Proteome characteristics of non-alcoholic steatohepatitis liver tissue and associated hepatocellular carcinomas, Int J Mol Sci, 18, 434, 10.3390/ijms18020434 Liu, 2016, Lack of fibroblast growth factor 21 accelerates metabolic liver injury characterized by steatohepatities in mice, Am J Cancer Res, 6, 1011 Tian, 2015, Histone deacetylase HDAC8 promotes insulin resistance and β-catenin activation in NAFLD-associated hepatocellular carcinoma, Cancer Res, 75, 4803, 10.1158/0008-5472.CAN-14-3786 Zhang, 2018, Anticancer action of lactucopicrin in SKMEL-5 human skin cancer cells is mediated via apoptosis induction, G2/M cell cycle arrest and downregulation of m-TOR/PI3K/AKT signalling pathway, J Buon, 23, 224 Maeyashiki, 2017, HADHA, the alpha subunit of the mitochondrial trifunctional protein, is involved in long-chain fatty acid-induced autophagy in intestinal epithelial cells, Biochem Biophys Res Commun, 484, 636, 10.1016/j.bbrc.2017.01.159 Banasik, 2011, Bioinformatics-driven identification and examination of candidate genes for non-alcoholic fatty liver disease, PLoS One, 6, e16542, 10.1371/journal.pone.0016542 Liao, 2015, Effect of high-fat diet on hepatic proteomics of hamsters, J Agric Food Chem, 63, 1869, 10.1021/jf506118j Stojsavljević, 2014, Adipokines and proinflammatory cytokines, the key mediators in the pathogenesis of nonalcoholic fatty liver disease, World J Gastroenterol, 20, 18070, 10.3748/wjg.v20.i48.18070 Menghini, 2012, TIMP3 overexpression in macrophages protects from insulin resistance, adipose inflammation, and nonalcoholic fatty liver disease in mice, Diabetes, 61, 454, 10.2337/db11-0613 Casagrande, 2017, Hepatocyte specific TIMP3 expression prevents diet dependent fatty liver disease and hepatocellular carcinoma, Sci Rep, 7, 6747, 10.1038/s41598-017-06439-x Zhou, 2022, Phillygenin ameliorates nonalcoholic fatty liver disease via TFEB-mediated lysosome biogenesis and lipophagy, Phytomedicine, 103, 154235, 10.1016/j.phymed.2022.154235 Khambu, 2018, Autophagy in non-alcoholic fatty liver disease and alcoholic liver disease, Liver Res, 2, 112, 10.1016/j.livres.2018.09.004 Park, 2015, Concerted action of p62 and Nrf2 protects cells from palmitic acid-induced lipotoxicity, Biochem Biophys Res Commun, 466, 131, 10.1016/j.bbrc.2015.08.120 Cho, 2018, Lipotoxicity induces hepatic protein inclusions through TANK binding kinase 1-mediated p62/sequestosome 1 phosphorylation, Hepatology, 68, 1331, 10.1002/hep.29742 Li, 2019, Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations, Autophagy, 15, 113, 10.1080/15548627.2018.1509818