Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors

Brazilian Archives of Biology and Technology - Tập 52 Số spe - Trang 159-170 - 2009
Sandra Regina B. R. Sella1, Belquis P. Guizelini2, Luciana Porto de Souza Vandenberghe2, Adriane Bianchi Pedroni Medeiros2, Carlos Ricardo Soccol3
1Piraquara, Parana, Centro de Producao e Pesquisa de Imunobiologicos, Secretaria de Estado da Saude, Brasil, Curitiba, Parana, Universidade Federal do Parana, Departamento de Engenharia de Bioprocessos e Biotecnologia, Departamento de Engenharia, Brazil
2Curitiba, Parana, Universidade Federal do Parana, Departamento de Engenharia de Bioprocessos e Biotecnologia, Departamento de Engenharia, Brazil
3Piraquara, Parana, Centro de Producao e Pesquisa de Imunobiologicos, Secretaria de Estado da Saude, Brasil

Tóm tắt

Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on the spore yield. The initial moisture had a significant effect depending on the bioreactor type. Sporulation kinetic's assay was carried out and it showed the possibility to reduce the time of spore formation in two days.

Từ khóa


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