La infección de hepatocitos de rata en cultivo con adenovirus recombinantes causa un drástico descenso en la secreción de lipoproteínas de muy baja densidad

Jover, 2001, Cytochrome p450 regulation by hepatocyte nuclear factor 4 in human hepatocytes: a study using adenovirus-mediated antisense targeting, Hepatology, 33, 668, 10.1053/jhep.2001.22176 Paddison, 2002, Stable suppression of gene expression by RNAi in mammalian cells, Proc Natl Acad Sci USA, 99, 1443, 10.1073/pnas.032652399 Andrews, 2001, Generation and characterization of E1/E2a/E3/E4-Deficient Adenoviral Vectors Encoding Human Factor VIII, Mol Ther, 3, 329, 10.1006/mthe.2001.0264 Liu, 2003, Molecular basis of the inflammatory response to adenovirus vectors, Gene Ther, 10, 935, 10.1038/sj.gt.3302036 Muruve, 2004, The innate immune response to adenovirus vectors, Hum Gene Ther, 15, 1157, 10.1089/hum.2004.15.1157 Herz, 1993, Adenovirus-mediated transfer of low density lipoprotein receptor gene acutely accelerates cholesterol clearance in normal mice, Proc Natl Acad Sci USA, 90, 2812, 10.1073/pnas.90.7.2812 Nunes, 1999, Gene transfer into the liver of nonhuman primates with E1-deleted recombinant adenoviral vectors: safety and readministration, Hum Gene Ther, 10, 2515, 10.1089/10430349950016852 Gómez-Foix, 1992, Adenovirus-mediated transfer of the muscle glycogen phosphorylase gene into hepatocytes confers altered regulation of glycogen metabolism, J Biol Chem, 267, 25129, 10.1016/S0021-9258(19)74015-0 Jaffe, 1992, Adenovirus-mediated in vivo gene transfer and expression in normal rat liver, Nat Genet, 1, 372, 10.1038/ng0892-372 Gibbons, 2000, Mobilisation of triacylglicerol stores, Biochim Biophys Acta, 1483, 37, 10.1016/S1388-1981(99)00182-1 Diraison, 1998, Role of human liver lipogenesis and reesterification in triglycerides secretion and in FFA reesterification, Am J Physiol, 274, E321 Fisher, 2002, Complexity in the secretory pathway: the assembly and secretion of apolipoprotein B-containing lipoproteins, J Biol Chem, 277, 17377, 10.1074/jbc.R100068200 Rubbia-Brandt, 2000, Hepatocyte steatosis is a cytopathic effect of hepatitis C virus genotype 3, J Hepatol, 33, 106, 10.1016/S0168-8278(00)80166-X McLauchlan, 2000, Properties of the hepatitis C virus core protein: a structural protein that modulates cellular processes, J Viral Hepatol, 7, 2, 10.1046/j.1365-2893.2000.00201.x Perlemuter, 2002, Hepatitis C virus core protein inhibits microsomal tryglyceride transfer protein activity and very low density lipoprotein secretion: a model of viral-related steatosis, FASEB J, 16, 185, 10.1096/fj.01-0396com McGrory, 1988, A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5, Virology, 163, 614, 10.1016/0042-6822(88)90302-9 Gluzman, 1982, Helper free adenovirus type 5 vectors, 187 Parker, 1979, Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA, J Virol, 31, 360, 10.1128/JVI.31.2.360-369.1979 Becker, 1994, Use of recombinant adenovirus for metabolic engineering of mammalian cells, Meth Cell Biol, 43, 161, 10.1016/S0091-679X(08)60603-2 Ruiz, 1990, Glucagon –and dibutyryl cyclic AMP– produced inhibition of cholesterol ester hydrolase in isolated rat hepatocytes: role of calcium, J Biochem, 107, 474, 10.1093/oxfordjournals.jbchem.a123070 Seglen, 1976, Preparation of isolated rat liver cells, Meth Cell Biol, 13, 29, 10.1016/S0091-679X(08)61797-5 Bergmeyer, 1974, Lactate dehydrogenase. UV assay with pyruvate and NADH, vol. 2, 574 Bradford, 1976, A rapid and sensitive meted for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding, Anal Biochem, 72, 248, 10.1016/0003-2697(76)90527-3 Cristobal, 1999, Purification and properties of a cholesteryl ester hydrolase from rat liver microsomes, J Lipid Res, 40, 715, 10.1016/S0022-2275(20)32151-9 Havel, 1955, The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum, J Clin Invest, 34, 1345, 10.1172/JCI103182 Folch, 1957, A simple method for the isolation and purification of total lipids from animal tissues, J Biol Chem, 226, 497, 10.1016/S0021-9258(18)64849-5 Ruiz, 1997, Quantification in the subnanomolar range of phospholipids and neutral lipids by monodimensional thin-layer chromatography and image analysis, J Lipid Res, 38, 1482, 10.1016/S0022-2275(20)37430-7 Vance, 1984, Isolation and analysis of lipoproteins secreted by rat liver hepatocytes, Biochim Biophys Acta, 792, 39, 10.1016/0005-2760(84)90280-7 Morral, 2002, Lethal toxicity, severe endothelial injury, and a threshold effect with high doses of an adenoviral vector in baboons, Hum Gene Ther, 13, 143, 10.1089/10430340152712692 Ishibashi, 1993, Hypercholesterolemia in low density lipoprotein receptor knockout mice and its reversal by adenovirus-mediated gene delivery, J Clin Invest, 92, 882, 10.1172/JCI116663 Pandak, 2001, Effects of CYP7A1 overexpression on cholesterol and bile acid homeostasis, Am J Physiol Gastrointest Liver Physiol, 281, G878, 10.1152/ajpgi.2001.281.4.G878 Pandak, 2002, Transport of cholesterol into mitochondria is rate-limiting for bile acid synthesis via the alternative pathway in primary rat hepatocytes, J Biol Chem, 277, 48158, 10.1074/jbc.M205244200 Crooke, 2005, An apolipoprotein B antisense oligonucleotide lowers LDL cholesterol in hyperlipidemic mice without causing hepatic steatosis, J Lipid Res, 46, 872, 10.1194/jlr.M400492-JLR200