Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009

International Journal of Systematic and Evolutionary Microbiology - Tập 61 Số 9 - Trang 2304-2310 - 2011
Mathias von Jan1, Nicole Riegger2, Gabriele Pötter1, Peter Schümann1, Susanne Verbarg1, Cathrin Spröer1, Manfred Rohde3, Bettina Lauer2, David P. Labeda4, Hans‐Peter Klenk1
1DSMZ – German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany
2Microbiology, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany
3HZI – Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany
4National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL 61604, USA

Tóm tắt

A Gram-positive, spore-forming, aerobic, filamentous bacterium, strain JFMB-ATET, was isolated in 2008 during environmental screening of a plastic surface in grade C in a contract manufacturing organization in southern Germany. The isolate grew at temperatures of 25–50 °C and at pH 5.0–8.5, forming ivory-coloured colonies with sparse white aerial mycelia. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the family Thermoactinomycetaceae, except that the cell-wall peptidoglycan contained ll-diaminopimelic acid, while all previously described members of this family display this diagnostic diamino acid in meso-conformation. The DNA G+C content of the novel strain was 54.6 mol%, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major fatty acids had saturated C14–C16 branched chains. No diagnostic sugars were detected. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel genus and species, Kroppenstedtia eburnea gen. nov. sp. nov. The type strain is JFMB-ATET ( = DSM 45196T  = NRRL B-24804T  = CCUG 59226T).

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Thông tin xuất bản

International Journal of Systematic and Evolutionary Microbiology

Tập 61 Số 9

2304-2310

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