Knock-down of OsLOX by RNA interference leads to improved seed viability in rice

Journal of Plant Biology - Tập 58 - Trang 293-302 - 2015
Suyang Bai1, Niqing He1, Lu Zhou1, Beibei Shen1, Wei Wu1, Xi Liu1, Ling Jiang1, Jianmin Wan1
1National Key Laboratory for Crop Genetics & Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Research Center of Plant Gene Engineering, Nanjing, Jiangsu, China

Tóm tắt

Previous work found that lipoxygenases were key enzymes in lipid peroxidation, which causes grain deterioration during storage. In order to obtain better seed viability in rice, 10 marker-free knock-down lines were obtained in the progeny of endogenous OsLOX knock-down mutations caused by the RNAi technology. After artificial accelerated aging, there were four types of knock-down lines with higher seed viability than wild type (receptor parent). OsLOX3 knock-down line NPF1 was of special interest. In a series of experiments, including Southern blots, analysis of OsLOX3 expression, and enzymatic activity, NPF1 had better seed viability than wild-type. We also investigated the main agronomic characters of both knock-down lines and non-transgenetic wild type families. Knock-down lines were identified with generally excellent agronomic characteristics similar to the wild-type.

Tài liệu tham khảo

Andreou A, Feussner I (2009) Lipoxygenases - structure and reaction mechanism. Phytochemistry 70:1504–1510 Basra AS, Singh B, Malik CP (1994) Amelioration of the effects of ageing in onion seeds by osmotic priming and associated changes in oxidative metabolism. Biol Plantarum 36:365–371 Brash A (1999) Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate. J Biol Chem 274:23679–23682 Daley M, Knauf VC, Summerfelt KR, Turner JC (1998) Cotransformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17:489–496 Feussner I, Wasternack C (2002) The lipoxygenase pathway. Annu Rev Plant Biol 53:275–297 Gao X, Shim WB, Gobel C, Kunze S, Feussner I, Meeley R, Balint-Kurti P, Kolomiets M (2007) Disruption of a maize 9-lipoxygenase results in increased resistance to fungal pathogens and reduced levels of contamination with mycotoxin fumonisin. Mol Plant Microbe In 20:922–933 Gayen D, Ali N, Ganguly M, Paul S, Datta K, Datta SK (2014) RNAi mediated silencing of lipoxygenase gene to maintain rice grain quality and viability during storage. Plant Cell Tiss Org 118:229–243 Huang J, Cai M, Long Q, Liu L, Lin Q, Jiang L, Chen S, Wan J (2014) OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity. Transgenic Res 23:643–655 Ida S, Masaki Y, Morita Y (1983) The isolation of multiple forms and product specificity of rice lipoxygenase. Agric Biol Chem 47:637–641 Joo YC, Oh DK (2012) Lipoxygenases: potential starting biocatalysts for the synthesis of signaling compounds. Biotechnol Adv 30:1524–1532 Juliano BO (1971) A simplified assay for milled-rice amylose. J Cereal Sci 16:334–340 Kumar D, Mishra DK (2014) Variability in permeability and integrity of cell membrane and depletion of food reserves in neem (Azadirachta indica) seeds from trees of different age classes. J Forest Res-JPN 25:147–153 Lenis JM, Gillman JD, Lee JD, Shannon JG, Bilyeu KD (2010) Soybean seed lipoxygenase genes: molecular characterization and development of molecular marker assays. Theor Appl Genet 120:1139–1149 Li H, Jiang L, Youn JH, Sun W, Cheng Z, Jin T, Ma X, Guo X, Wang J, Zhang X, Wu F, Wu C, Kim SK, Wan J (2013) A comprehensive genetic study reveals a crucial role of CYP90D2/D2 in regulating plant architecture in rice (Oryza sativa). New Phytol 200:1076–1088 Long Q, Zhang W, Wang P, Shen W, Zhou T, Liu N, Wang R, Jiang L, Huang J, Wang Y, Liu Y, Wan J (2013) Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity. J Plant Biol 56:232–242 Malik AR, Shamet GS, Butola JS, Bhat GM, Mir AA, Nabi G (2013) Standardization of seed storage conditions in chilgoza pine (Pinus gerardiana Wall.): an endangered pine of Hindu Kush Himalaya. Trees 27:1497–1501 Matthews PR, Wang M, Waterhouse PM, Thornton S, Fieg SJ, Gubler F, Jacobsen JV (2001) Marker gene elimination from transgenic barley, using co-transformation with adjacent ‘twin TDNAs’ on a standard Agrobacterium transformation vector. Mol Breeding 7:195–202 Mizuno K, Iida T, Takano A, Yokoyama M, Fujimura T (2003) A new 9-lipoxygenase cDNA from developing rice seeds. Plant Cell Physiol 44:1168–1175 Mosblech A, Feussner I, Heilmann I (2009) Oxylipins: structurally diverse metabolites from fatty acid oxidation. Plant Physiol Biochem 47:511–517 Ohta H, Shirano Y, Tanaka K, Morita Y, Shibata D (1992) cDNA Cloning of rice lipoxygenase L-2 and characterization using an active enzyme expressed from the cDNA in Escherichia-coli. Eur J Biochem 206:331–336 Shirasawa K, Takeuchi Y, Ebitani T, Suzuki Y (2008) Identification of a gene for rice (Oryza sativa) seed lipoxygenase-3 involved in the generation of stale flavor and development of SNP markers for lipoxygenase-3 deficiency. Breeding Sci 58:169–176 Specht CE, RBorner A (1998) An interim report on a long term storage experiment on rye (Secale cereale L.) under a range of temperatures and atmospheres. Genet Resour Crop Ev 45:483–488 Suszka J, Plitta BP, Michalak M, Bujarska-Borkowska B, Tylkowski T, Chmielarz P (2014) Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm. Ann For Sci 71:543–549 Suzuki Y, Ise K, Li C, Honda I, Iwai Y, Matsukura U (1999) Volatile components in stored rice [Oryza sativa (L.)] of varieties with and without lipoxygenase-3 in seeds. J Agr Food Chem 47:1119–1124 Suzuki Y, Nagamine T, Okuno K (1996) Genetic analysis of a nullallele for lipoxygenase-3 in rice seeds. Euphytica 91:99–101 Szymanowska U, Jakubczyk A, Baraniak B, Kur A (2009) Characterisation of lipoxygenase from pea seeds (Pisum sativum var. Telephone L.). Food Chem 116: 906–910 Varghese B, Naithani SC (2002) Desiccation-induced changes in lipid peroxidation, superoxide level and antioxidant enzymes activity in neem (Azadirachta indica A. Juss) seeds. Acta Physiol Plant 24:79–87 Vickery HB (1946) The early years of the Kjeldahl method to determine nitrogen. Yale J Biol Med 18:473–516 Wang R, Shen W, Liu L, Jiang L, Liu Y, Su N, Wan J (2008) A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. Plant Mol Biol 66:401–414 Warthmann N, Chen H, Ossowski S, Weigel D, Herve P (2008) Highly specific gene silencing by artificial miRNAs in rice. PLoS ONE 3:e1829 Woo H, Suh S, Cho Y (2011) Strategies for developing marker-free transgenic plants. Biotechnol Bioproc E 16:1053–1064 Zhao P, Zhu Y, Wang W (2010) Evaluation and improvement of spectrophotometric assays of TTC reduction: maize (Zea mays) embryo as an example. Acta Physiol Plant 32:815–819 Zhang Y, Yu Z, Lu Y, Wang Y, She D, Song M, Wu Y (2007) Effect of the absence of lipoxygenase isoenzymes on the storage characteristics of rice grains. J Stored Prod Res 43:87–91