Kinetic study of collagen fixation with polyepoxy fixatives
Tóm tắt
A new biomaterial has been developed by fixing native collagen with a polyepoxy compound (PC) fixative. In this study, bovine internal thoracic arteries were fixed with PC under various conditions to help understand the kinetics of the collagen–PC reactions and optimize the fixation process. At predetermined time intervals, small samples were cut from the arteries to determine the quantities of the remaining unreacted amino acids in the collagen. Temperature, concentration, and solution pH were among the key parameters studied. The overall fixation rate was found to be reaction‐rate controlled, as the rate of fixation was relatively slow compared with the rate of diffusion of PC. As might be expected, the reaction rate was favored by a higher temperature, concentration, and solution pH. A kinetic model, with a 2.5th reaction order with respect to the reactive functional groups of collagen and a first order with respect to PC, was developed that gave a good fit to the experimental data. Based on this model, the degree of fixation,
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Tài liệu tham khảo
Nimni M. E., 1988, Collagen, 1
Carpentier A., 1969, Biologic factors affecting longterm results of valvular heterografts, J. Thorac. Cardiovasc. Surg., 55, 467, 10.1016/S0022-5223(19)42561-0
Sawyer P. N., 1983, Vascular grafting—clinical applications and techniques, 331
Rosenberg D., 1978, Vascular Grafts, 261
Noijiri C., 1987, Aortacoronary bypass grafting with heparinized vascular grafts in dogs, J. Thorac. Cardiovasc. Surg., 93, 867, 10.1016/S0022-5223(19)37047-3
Masuoka M., 1985, Effect of catalysis on epoxy tannage, Leather Chem., 30, 223
Y.Noishiki K.Kodaira M.Furuse andT.Miyata “Method of preparing antithrombogenic medical materials ” U. S. Patent 4 806 595 1989.
Imamura E., 1988, Anticalcification effects of epoxy compounds as cross‐linking agent, Jpn. J. Artif. Organs, 17, 1101
Lee J. M., 1991, Cross‐linking of a prototype bovine artery xenograft: Comparison of the effects of glutaraldehyde and four poly(glycidyl ether) compounds, Artif. Organs, 15, 303
McIntyre J., 1991, Biomedical properties of a new biomaterial—a polyepoxy tanned bovine artery, Trans. Soc. Biomater., 14, 119
Tu R., 1991, Dynamic internal compliance of a vascular prosthesis, Trans. Am. Soc. Artif. Int. Organs, 37, 470
J.Lohre L.Baclig J.Sagartz S.Guida andR.Tu “Evaluation of two diepoxy ether compounds for biocompatible potential ” presented at the VIII World Congress of the ISAO Montreal Canada August 19–22 1991.
H.Sung T.Witzel C.Hata R.Tu andR. C.Quijano “Nonivasive color Doppler inspection of small‐diameter vascular grafts implanted in canine carotid and femoral arteries ” presented at the VIII World Congress of the ISAO Montreal Canada August 19–22 1991.
C.Hata E.Wang Y.Noishiki Y.Tomizawa R.Tu andR. C.Quijano “Evaluation of polyepoxy compound fixed biological vascular prostheses and PTFE vascular grafts ” presented at the VIII World Congress of the ISAO Montreal Canada August 19–22 1991.
Noishiki Y., 1986, Development of a small caliber vascular graft by a new cross‐linking method incorporating slow heparin release collagen and natural tissue compliance, Trans. Am. Soc. Artif. Int. Organs, 32, 114
Tomizawa Y., 1990, Development of a small caliber biologic vascular graft: Evaluation of its antithrombogenicity and the early healing process, Trans. Am. Soc. Artif. Int. Organs, 36, 734
Yamauchi M., 1988, Collagen, 157
Payne J. W., 1973, Polymerization of proteins with glutaraldehyde, J. Biochem., 135, 867, 10.1042/bj1350867
Heidemann E., 1988, Collagen, 39
R.Tu C.‐L.Lu K.Thyagarajan E.Wang H.Nguyen andR.Quijano “Kinetics of fixation of bovine collagens with polyepoxy compounds ” presented at 1991 ASAIO meeting Chicago IIIinois April 25–27 1991.
R.Tu C.‐L.Lu K.Thyagarajan E.Wang H.Nguyen andR. C.Quijano “Kinetic study of collagen cross‐linking using a new fixative—polyepoxy compounds ” presented at 1991 Society For Biomaterials meeting Scottsdale Arizona May 1–5 1991.
Levenspiel O., 1972, Chemical reaction engineering, 35
March J., 1985, Advanced organic chemistry—reactions, mechanisms, and structure, 368
Morrison R. T., 1986, Organic Chemistry, 548