Kerosene tolerance in Achromobacter and Pseudomonas species
Tóm tắt
The aim of the present study was to investigate the tolerance of five new Achromobacter and Pseudomonas strains to kerosene and to establish if the production of several secondary metabolites increases or not when these bacteria were grown in the presence of kerosene. The biodegradation of kerosene by isolated bacteria was also investigated in this study. Five Proteobacteria were isolated from different samples polluted with petroleum and petroleum products. Based on their morphological, biochemical, and molecular characteristics, isolated bacteria were identified as Achromobacter spanius IBBPo18 and IBBPo21, Pseudomonas putida IBBPo19, and Pseudomonas aeruginosa IBBPo20 and IBBPo22. All these bacteria were able to tolerate and degrade kerosene. Higher tolerance to kerosene and degradation rates were observed for P. aeruginosa IBBPo20 and IBBPo22, compared with that observed for A. spanius IBBPo18 and IBBPo21, and P. putida IBBPo19. All these bacteria were able to produce several secondary metabolites, such as surfactants and pigments. Glycolipid surfactants produced by P. aeruginosa IBBPo20 and IBBPo22, A. spanius IBBPo18 and IBBPo21, and P. putida IBBPo19 have a very good emulsification activity, and their activity increased when they were grown in the presence of kerosene. The production of rhamnolipid surfactants by P. aeruginosa IBBPo20 and IBBPo22 was confirmed by detection of rhlAB gene involved in their biosynthesis. Pyocyanin and pyoverdin pigments were produced only by P. aeruginosa IBBPo20 and IBBPo22, while carotenoid pigments were produced by all the isolated bacteria. Significant changes in pigments production were observed when P. aeruginosa IBBPo20 and IBBPo22, A. spanius IBBPo18 and IBBPo21, and P. putida IBBPo19 were grown in the presence of kerosene. Due to their ability to tolerate and degrade kerosene, and also to produce several secondary metabolites, the isolated bacteria could be used in the bioremediation of kerosene-polluted environments.
Tài liệu tham khảo
Abdel-Mawgoud AM, Aboulwafa MM, Hassouna NAH (2009) Characterization of rhamnolipid produced by Pseudomonas aeruginosa isolate Bs20. Appl Biochem Biotechnol 157:329–345
Ahamed F, Hasibullah M, Ferdouse J, Anwar MN (2010) Microbial degradation of petroleum hydrocarbon. Bangladesh J Microbiol 27:10–13
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25:3389–3402
Beuttler H, Hoffmann J, Jeske M, Hauer B, Schmid RD, Altenbuchner J, Urlacher VB (2011) Biosynthesis of zeaxanthin in recombinant Pseudomonas putida. Appl Microbiol Biotechnol 89:1137–1147
Chikere CB, Okpokwasili GC, Chikere BO (2011) Monitoring of microbial hydrocarbon remediation in the soil. 3. Biotech 1:117–138
Chrzanowski Ł, Ławniczak Ł, Czaczyk K (2012) Why do microorganisms produce rhamnolipids? World J Microbiol Biotechnol 28:401–419
Eberlin LS, Abdelnur PV, Passero A, de Sa GF, Daroda RJ, de Souza V, Eberlin MN (2009) Analysis of biodiesel and biodiesel-petrodiesel blends by high performance thin layer chromatography combined with easy ambient sonic-spray ionization mass spectrometry. Analyst 134:1652–1657
El-Fouly MZ, Sharaf AM, Shahin AAM, El-Bialy Heba A, Omara AMA (2015) Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa. J Rad Res Appl Sci 8:36–48
Gesheva V, Stackebrandt E, Vasileva-Tonkova E (2010) Biosurfactant production by halotolerant Rhodococcus fascians from Casey Station, Wilkes Land, Antarctica. Curr Microbiol 61:112–117
Gulati D, Mehta S (2017) Isolation and identification of petrol degrading microorganisms from contaminated soil and comparison of their bioremediation potential. Int Res J Pharm 8:34–38
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s Manual of Determinative Bacteriology, 9th edn. Williams and Wilkins, Baltimore
Jensen V, Löns D, Zaoui C, Bredenbruch F, Meissner A, Dieterich G, Münch R, Häussler S (2006) RhlR expression in Pseudomonas aeruginosa is modulated by the Pseudomonas quinolone signal via PhoB-dependent and -independent pathways. J Bacteriol 188:8601–8606
Joy S, Rahman PKSM, Sharma S (2017) Biosurfactant production and concomitant hydrocarbon degradation potentials of bacteria isolated from extreme and hydrocarbon contaminated environments. Chem Eng J 317:232–241
King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44:301–307
Leahy JG, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54:305–315
Malik K, Tokkas J, Goyal S (2012) Microbial pigments: a review. Int J Microbial Resource Technol 1:361–365
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Wade WG (1998) Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795–799
Matilla MA (2018) Problems of solventogenicity, solvent tolerance: an introduction. In: Krell T (ed) Cellular Ecophysiology of Microbe: Hydrocarbon and Lipid Interactions, Handbook of Hydrocarbon and Lipid Microbiology. Springer, Cham, pp 327–334
Mazumdar A, Deka M, Hazarika DJ (2015) Degradation of kerosene hydrocarbon by indigenous diazotrophic bacteria isolated from crude oil contaminated soil. Int J Bioassays 4:4184–4188
Medina G, Juárez K, Valderrama B, Soberón-Chávez G (2003) Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol 185:5976–5983
Michaud L, Di Cello F, Brilli M, Fani R, Lo Giudice A, Bruni V (2004) Biodiversity of cultivable Antarctic psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea). FEMS Microbiol Lett 230:63–71
Mnif S, Chamkha M, Labat M, Sayadi S (2011) Simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria. J Appl Microbiol 111:525–536
Niewerth H, Bergander K, Chhabra SR, Williams P, Fetzner S (2011) Synthesis and biotransformation of 2-alkyl-4(1H)-quinolones by recombinant Pseudomonas putida KT2440. Appl Microbiol Biotechnol 91:1399–1408
Pacwa-Płociniczak M, Anna PG, Poliwoda A, Piotrowska-Seget Z (2014) Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil. Environ Sci Pollut Res 21:9385–9395
Patowary K, Patowary R, Kalita MC, Deka S (2016) Development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites. Front Microbiol 7:1092 https://doi.org/10.3389/fmicb.2016.01092
Pini F, Grossi C, Nereo S, Michaud L, Lo Giudice A, Bruni V, Baldi F, Fani R (2007) Molecular and physiological characterisation of psychrotrophic hydrocarbon-degrading bacteria isolated from Terra Nova Bay (Antarctica). Eur J Soil Biol 43:368–379
Provvedi R, Kocíncová D, Doná V, Euphrasie D, Daffé M, Etienne G, Manganelli R, Reyrat JM (2008) SigF controls carotenoid pigment production and affects transformation efficiency and hydrogen peroxide sensitivity in Mycobacterium smegmatis. J Bacteriol 190:7859–7863
Reen FJ, Mooij MJ, Holcombe LJ, McSweeney CM, McGlacken GP, Morrissey JP, O'Gara F (2011) The Pseudomonas quinolone signal (PQS), and its precursor HHQ, modulate interspecies and interkingdom behaviour. FEMS Microbiol Ecol 77:413–428
Rikalović MG, Vrvić MM, Karadžić IM (2015) Rhamnolipid biosurfactant from Pseudomonas aeruginosa - from discovery to application in contemporary technology. J Serb Chem Soc 80:279–304
Ritchie G, Still K, Rossi J, Bekkedal M, Bobb A, Arfsten D (2003) Biological and health effects of exposure to kerosene-based jet fuels and performance additives. J Toxicol Environ Health B Crit Rev 6:357–451
Rocha CA, Pedregosa AM, Laborda F (2011) Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925. AMB Express 1:9 https://doi.org/10.1186/2191-0855-1-9
Sajilata MG, Singhal RS, Kamat MY (2008) The carotenoid pigment zeaxanthin - a review. Comp Rev Food Sci Food Saf 7:29–49
Sambrook J, Russel D (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sardessai YN, Bhosle S (2004) Industrial potential of organic solvent tolerant bacteria. Biotechnol Prog 20:655–660
Satpute SK, Bhawsar BD, Dhakephalkar PK, Chopade BA (2008) Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian J Marine Sci 37:243–250
Shahzadi S, Khan Z, Rehman A, Nisar MA, Hussain SZ, Asma ST (2019) Isolation and characterization of Bacillus amyloliquefaciens 6A: a novel kerosene oil degrading bacterium. Environ Technol Innov 14:100359 https://doi.org/10.1016/j.eti.2019.100359
Siegmund I, Wagner F (1991) New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar. Biotechnol Techn 5:265–268
Silva RMP, Rodriguez AA, de Oca JMGM, Moreno DC (2006) Biodegradation of crude oil by Pseudomonas aeruginosa AT18 strain. Tecnol Quimica 26:70–77
Silva SNRL, Farias CBB, Rufino RD, Luna JM, Sarubbo LA (2010) Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992. Coll Surf B Biointerfaces 79:174–183
Stancu MM (2018) Production of some extracellular metabolites by a solvent-tolerant Pseudomonas aeruginosa strain. Waste Biomass Valori 9:1747–1755
Stancu MM, Grifoll M (2011) Multidrug resistance in hydrocarbon-tolerant Gram-positive and Gram-negative bacteria. J Gen Appl Microbiol 57:1–18
Usman HM, Abdulkadir N, Gani M, Maiturare HM (2017) Bacterial pigments and its significance. MOJ Bioequiv Availab 4:285–288
Varjani SJ (2017) Microbial degradation of petroleum hydrocarbons. Biores Technol 223:277–286
Xu X, Liu W, Tian S, Wang W, Qi Q, Jiang P, Gao X, Li F, Li H, Yu H (2018) Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: a perspective analysis. Front Microbiol 9:2885 https://doi.org/10.3389/fmicb.2018.02885