KbvR mutant of Klebsiella pneumoniae affects the synthesis of type 1 fimbriae and provides protection to mice as a live attenuated vaccine

Bagley ST (1985) Habitat association of Klebsiella species. Infect Control 6:52–58 Melo-Nascimento A, Treumann C, Neves C, Andrade E, Andrade AC, Edwards R, Dinsdale E, Bruce T (2018) Functional characterization of ligninolytic Klebsiella spp. strains associated with soil and freshwater. Arch Microbiol 200:1267–1278 Poudel A, Hathcock T, Butaye P, Kang Y, Price S, Macklin K, Walz P, Cattley R, Kalalah A, Adekanmbi F, Wang C (2019) Multidrug-resistant Escherichia coli, Klebsiella pneumoniae and Staphylococcus spp. in houseflies and blowflies from farms and their environmental settings. Int J Environ Res Public Health 16:3583 Ribeiro MG, de Morais ABC, Alves AC, Bolaños CAD, de Paula CL, Portilho FVR, de Nardi Júnior G, Lara GHB, de Souza Araújo Martins L, Moraes LS, Risseti RM, Guerra ST, Bello TS, Siqueira AK, Bertolini AB, Rodrigues CA, Paschoal NR, de Almeida BO, Listoni FJP, Sánchez LFG, Paes AC (2022) Klebsiella-induced infections in domestic species: a case-series study in 697 animals (1997–2019). Braz J Microbiol 53:455–464 Twenhafel NA, Whitehouse CA, Stevens EL, Hottel HE, Foster CD, Gamble S, Abbott S, Janda JM, Kreiselmeier N, Steele KE (2008) Multisystemic abscesses in African green monkeys (Chlorocebus aethiops) with invasive Klebsiella pneumoniae–identification of the hypermucoviscosity phenotype. Vet Pathol 45:226–231 Williamson S (2017) Klebsiella pneumoniae septicaemia in preweaned pigs. Vet Rec 180:443 Hertl JA, Schukken YH, Welcome FL, Tauer LW, Gröhn YT (2014) Pathogen-specific effects on milk yield in repeated clinical mastitis episodes in Holstein dairy cows. J Dairy Sci 97:1465–1480 Hu Y, Anes J, Devineau S, Fanning S (2021) Klebsiella pneumoniae: prevalence, reservoirs, antimicrobial resistance, pathogenicity, and infection: a hitherto unrecognized zoonotic bacterium. Foodborne Pathog Dis 18:63–84 Huynh BT, Passet V, Rakotondrasoa A, Diallo T, Kerleguer A, Hennart M, Lauzanne A, Herindrainy P, Seck A, Bercion R, Borand L, de la Pardos M, Delarocque-Astagneau E, Guillemot D, Vray M, Garin B, Collard JM, Rodrigues C, Brisse S (2020) Klebsiella pneumoniae carriage in low-income countries: antimicrobial resistance, genomic diversity and risk factors. Gut Microbes 11:1287–1299 Rodrigues C, Hauser K, Cahill N, Ligowska-Marzęta M, Centorotola G, Cornacchia A, Garcia Fierro R, Haenni M, Nielsen EM, Piveteau P, Barbier E, Morris D, Pomilio F, Brisse S (2022) High prevalence of Klebsiella pneumoniae in European food products: a multicentric study comparing culture and molecular detection methods. Microbiol Spectr 10:e0237621 Thorpe H, Booton R, Kallonen T, Gibbon MJ, Couto N, Passet V, Fernandez JSL, Rodrigues C, Matthews L, Mitchell S, Reeve R, David S, Merla C, Corbella M, Ferrari C, Comandatore F, Marone P, Brisse S, Sassera D, Corander J, Feil EJ (2021) One Health or Three? Transmission modelling of Klebsiella isolates reveals ecological barriers to transmission between humans, animals and the environment. BioRxiv 8:455249 Wareth G, Neubauer H (2021) The Animal-foods-environment interface of Klebsiella pneumoniae in Germany: an observational study on pathogenicity, resistance development and the current situation. Vet Res 52:16 Carvalho I, Alonso CA, Silva V, Pimenta P, Cunha R, Martins C, Igrejas G, Torres C, Poeta P (2020) Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated from healthy and sick dogs in Portugal. Microb Drug Resist 26:709–715 Guerra B, Fischer J, Helmuth R (2014) An emerging public health problem: acquired carbapenemase-producing microorganisms are present in food-producing animals, their environment, companion animals and wild birds. Vet Microbiol 171:290–297 Harada K, Shimizu T, Mukai Y, Kuwajima K, Sato T, Usui M, Tamura Y, Kimura Y, Miyamoto T, Tsuyuki Y, Ohki A, Kataoka Y (2016) Phenotypic and molecular characterization of antimicrobial resistance in Klebsiella spp. isolates from companion animals in Japan: clonal dissemination of multidrug-resistant extended-spectrum β-lactamase-producing Klebsiella pneumoniae. Front Microbiol 7:1021 Ahmad TA, El-Sayed LH, Haroun M, El Hussein AA, El Ashry SH (2012) Development of immunization trials against Klebsiella pneumoniae. Vaccine. 30:2411–2420 Assoni L, Girardello R, Converso TR, Darrieux M (2021) Current stage in the development of Klebsiella pneumoniae vaccines. Infect Dis Ther 10:2157–2175 Babu L, Uppalapati SR, Sripathy MH, Reddy PN (2017) Evaluation of recombinant multi-epitope outer membrane protein-based Klebsiella pneumoniae subunit vaccine in mouse model. Front Microbiol 8:1805 Gorden PJ, Kleinhenz MD, Ydstie JA, Brick TA, Slinden LM, Peterson MP, Straub DE, Burkhardt DT (2018) Efficacy of vaccination with a Klebsiella pneumoniae siderophore receptor protein vaccine for reduction of Klebsiella mastitis in lactating cattle. J Dairy Sci 101:10398–10408 Hussein KE, Bahey-El-Din M, Sheweita SA (2018) Immunization with the outer membrane proteins OmpK17 and OmpK36 elicits protection against Klebsiella pneumoniae in the murine infection model. Microb Pathog 119:12–18 Lee WH, Choi HI, Hong SW, Kim KS, Gho YS, Jeon SG (2015) Vaccination with Klebsiella pneumoniae-derived extracellular vesicles protects against bacteria-induced lethality via both humoral and cellular immunity. Exp Mol Med 47:e183 Achkar JM (2019) Prospects and challenges of a new live tuberculosis vaccine. Lancet Respir Med 7:723–725 Demeure CE, Derbise A, Guillas C, Gerke C, Cauchemez S, Carniel E, Pizarro-Cerdá J (2019) Humoral and cellular immune correlates of protection against bubonic plague by a live Yersinia pseudotuberculosis vaccine. Vaccine 37:123–129 Derbise A, Guillas C, Gerke C, Carniel E, Pizarro-Cerdà J, Demeure CE (2020) Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine. Vaccine 38:1888–1892 Lalvani A, Sridhar S (2010) BCG vaccination: 90 years on and still so much to learn. Thorax 65:1036–1038 Pandey A, Cabello A, Akoolo L, Rice-Ficht A, Arenas-Gamboa A, McMurray D, Ficht TA, de Figueiredo P (2016) The case for live attenuated vaccines against the neglected zoonotic diseases Brucellosis and Bovine Tuberculosis. PLoS Negl Trop Dis 10:e0004572 Hsieh PF, Lin TL, Lee CZ, Tsai SF, Wang JT (2008) Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis 197:1717–1727 Xu L, Wang M, Yuan J, Wang H, Li M, Zhang F, Tian Y, Yang J, Wang J, Li B (2021) The KbvR regulator contributes to capsule production, outer membrane protein biosynthesis, antiphagocytosis, and virulence in Klebsiella pneumoniae. Infect Immun 89:e00016–21 Asadi Karam MR, Oloomi M, Mahdavi M, Habibi M, Bouzari S (2013) Vaccination with recombinant FimH fused with flagellin enhances cellular and humoral immunity against urinary tract infection in mice. Vaccine 31:1210–1216 Ramezanalizadeh F, Owlia P, Rasooli I (2020) Type I pili, CsuA/B and FimA induce a protective immune response against Acinetobacter baumannii. Vaccine 38:5436–5446 Rostamian M, Farasat A, Chegene Lorestani R, Nemati Zargaran F, Ghadiri K, Akya A (2022) Immunoinformatics and molecular dynamics studies to predict T-cell-specific epitopes of four Klebsiella pneumoniae fimbriae antigens. J Biomol Struct Dyn 40:166–176 Zargaran FN, Akya A, Rezaeian S, Ghadiri K, Lorestani RC, Madanchi H, Safaei S, Rostamian M (2021) B cell epitopes of four fimbriae antigens of Klebsiella pneumoniae: a comprehensive in silico study for vaccine development. Int J Pept Res Ther 27:875–886 Blumer C, Kleefeld A, Lehnen D, Heintz M, Dobrindt U, Nagy G, Michaelis K, Emödy L, Polen T, Rachel R, Wendisch VF, Unden G (2005) Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology 151:3287–3298 Kim KW, Jung WK, Park YH (2008) Comparison of pre-stain suspension liquids in the contrasting ability of neutralized potassium phosphotungstate for negative staining of bacteria. J Microbiol Biotechnol 18:1762–1767 Zhao X, Zeng X, Dai Q, Hou Y, Zhu D, Wang M, Jia R, Chen S, Liu M, Yang Q, Wu Y, Zhang S, Huang J, Ou X, Mao S, Gao Q, Zhang L, Liu Y, Yu Y, Cheng A (2021) Immunogenicity and protection efficacy of a Salmonella enterica serovar Typhimurium fnr, arcA and fliC mutant. Vaccine. 39:588–595 Kobayashi SD, Porter AR, Freedman B, Pandey R, Chen L, Kreiswirth BN, DeLeo FR (2018) Antibody-mediated killing of carbapenem-resistant ST258 Klebsiella pneumoniae by human neutrophils. mBio 9:e00297–e00218 Hu G, Chen X, Chu W, Ma Z, Miao Y, Luo X, Fu Y (2022) Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae. Vet Res 53:5 Cheng F, Li Z, Lan S, Liu W, Li X, Zhou Z, Song Z, Wu J, Zhang M, Shan W (2018) Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis. Braz J Microbiol 49:93–100 Frey J (2007) Biological safety concepts of genetically modified live bacterial vaccines. Vaccine 25:5598–5605 Fan Q, Sims T, Sojar H, Genco R, Page RC (2001) Fimbriae of Porphyromonas gingivalis induce opsonic antibodies that significantly enhance phagocytosis and killing by human polymorphonuclear leukocytes. Oral Microbiol Immunol 16:144–152 Starks CM, Miller MM, Broglie PM, Cubbison J, Martin SM, Eldridge GR (2021) Optimization and qualification of an assay that demonstrates that a FimH vaccine induces functional antibody responses in women with histories of urinary tract infections. Hum Vaccin Immunother 17:283–292 Thankavel K, Madison B, Ikeda T, Malaviya R, Shah AH, Arumugam PM, Abraham SN (1997) Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. J Clin Invest 100:1123–1136 Liu ZF, Chen JL, Li WY, Fan MW, Li YH (2019) FimH as a mucosal adjuvant enhances persistent antibody response and protective efficacy of the anti-caries vaccine. Arch Oral Biol 101:122–129 Witkowska D, Mieszała M, Gamian A, Staniszewska M, Czarny A, Przondo-Mordarska A, Jaquinod M, Forest E (2005) Major structural proteins of type 1 and type 3 Klebsiella fimbriae are effective protein carriers and immunogens in conjugates as revealed from their immunochemical characterization. FEMS Immunol Med Microbiol 45:221–230 Li B, Zhao Y, Liu C, Chen Z, Zhou D (2014) Molecular pathogenesis of Klebsiella pneumoniae. Future Microbiol 9:1071–1081 Banerjee K, Motley MP, Diago-Navarro E, Fries BC (2021) Serum antibody responses against carbapenem-resistant Klebsiella pneumoniae in infected patients. mSphere 6:e1335–e1320 Yeh FC, Yeh KM, Siu LK, Fung CP, Yang YS, Lin JC, Chang FY (2012) Increasing opsonizing and killing effect of serum from patients with recurrent K1 Klebsiella pneumoniae liver abscess. J Microbiol Immunol Infect 45:141–146