Karyotyping human chromosomes by combinatorial multi-fluor FISH

Gray, J.W. et al. Molecular cytogenetics: diagnosis and prognostic assessment. Curr. Opin. Biotech. 3, 623–631 (1992).

Xing, Y. & Lawrence, J.B. Molecular cytogenetics: applications of FISH to chromosomal aberrations in cancer genetics. In The causes and consequences of chromosomal aberrations, (ed. Kirsch, I.R.) 3–28 (CRC Press, BocaRaton, 1993).

Nederlof, P.M. et al. Three color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10, 20–27 (1989).

Nederlof, P.M. et al. Multiple fluorescence in situ hybridization. Cytometry 11, 126–131 (1990).

Ried, T., Baldini, A., Rand, T.C. & Ward, D.C. Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89, 1388–1392 (1992).

Dauwerse, J.G., Wiegant, J., Raap, A.K., Breuning, M.H. & van Ommen, G.J.B. Multiple colors by fluorescence in situ hybridization using ratio-labelled DNA probes create a molecular karyotype. Hum. Mol. Genet. 1, 593–598 (1992).

Nederlof, P.M., van der Flier, S., Vrolijk, J., Tanke, H.J. & Raap, A.K. Fluorescence ratio measurements of double-labeled probes for multiple in situ hybridization by digital imaging microscopy. Cytometry 13, 839–845 (1992).

du Manoir, S. et al. Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. Hum. Genet. 90, 590–610 (1993).

Ried, T., Landes, G., Dackowski, W., Klinger, K. & Ward, D.C. Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosomes 13, 18, 21, X and Y in uncultured amniotic fluid cells. Hum. Mol. Genet. 1, 307–313 (1992).

Lengauer, C. et al. Chromosomal bar codes constructed by fluorescence in situ hybridization with Alu-PCR products of multiple YAC clones. Hum. Mol. Genet. 2, 505–512 (1993).

Popp, S. et al. A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome specific DNA libraries and YAC clones. Hum. Genet. 92, 527–532 (1993).

Wiegant, J. et al. Multiple and sensitive fluorescence in situ hybridization with rhodamine-, fluorescein-, and coumarin-labeled DNAs. Cytogenet. Cell Genet. 63, 73–76 (1993).

Ernst, L.A., Gupta, R.K., Mujumdar, R.B. & Waggoner, A.S. Cyanine dye labeling reagents for sulfhydryl groups. Cytometry 10, 3–10 (1989).

Mujumdar, R.B., Ernst, L.A., Mujumbar, S.R. & Waggoner, A.S. Cyanine dye labeling reagents containing isothiocyanate groups. Cytometry 10, 11–19 (1989).

Yu, H. et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucl. Acids Res. 22, 3226–3232 (1994).

Waggoner, A. Covalent labeling of proteins and nucleic acids with fluorophores. Meth. Enzym. 246, 362–373 (1995).

Francke, U. Digitized and differentially shaded human chromosome ideograms for genomic applications. Cytogenet. Cell Genet. 65, 205–219 (1994).

Bray-Ward, P. et al. Integration of the cytogenetic, genetic and physical maps of the human genome by FISH mapping of CEPH YAC clones. Genomics (in the press).

Baldini, A. & Ward, D.C. In situ hybridzation banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. Genomics 9, 770–774 (1991).

Matera, A.G. & Ward, D.C. Oligonucleotide probes for the analysis of specific repetitive DNA sequences by fluorescence in situ hybridization. Hum. Mol. Genet. 1, 535–539 (1992).

Chumakov, I.M. et al. A YAC contig of the human genome. Nature 377, 175–299 (1995).

Hudson, T.J. et al. An STS-based map of the human genome. Science 270, 1945–1954 (1995).

Meltzer, P.S., Guan, X.Y., Burgess, A. & Trent, J. Rapid generation of region specific probes by chromosome microdissection and their application. Nature Genet. 1, 24–28 (1992).

Guan, X.Y., Trent, J.M. & Meltzer, P.S. Generation of band-specific painting probes from a single microdissected chromosome. Hum. Mol. Genet. 2, 1117–1121 (1993).

Guan, X.Y., Meltzer, P.S. & Trent, J.M. Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection. Genomics 22, 101–107 (1994).

Guan, X.Y., Meltzer, P.S., Burgess, A. & Trent, J.M. Complete coverage of chromosome 6 by chromosome microdissection: Generation of 14 band region-specific probes. Hum. Genet. 95, 637–640 (1995).

Guan, X.Y. et al. Chromosome arm painting probes. Nature Genet. 12, 10–11 (1996).

Ledbetter, D.H. The ‘colorizing’ of cytogenetics: is it ready for prime time? Hum. Mol. Genet. 5, 297–299 (1992).

Waggoner, A. et al. Multiple spectral parameter imaging. Meth. Cell. Biol. 30, 449–478 (1989).

du Manoir, S. et al. Quantitative analysis of .comparative genomic hybridization. Cytometry 9, 21–49 (1995).

Smith, T.G. Jr., Marks, W.B., Lange, G.D., Sheriff, W.H. & Neale, E.A. Edge detection in images using Marr-Hildreth filtering techniques. J. Neurosci. Meth. 26, 75–81 (1988).