Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

Animal Science Journal - Tập 90 Số 11 - Trang 1417-1425 - 2019
Xuerui Yao1,2, Hao Jiang1,3, Yinghua Li2, Qingshan Gao2, Nianyin Zeng2, Nam‐Hyung Kim1
1Chungbuk National University, Cheongju, Korea
2College of Agriculture, Yanbian University, Yanji, China
3College of Animal Sciences, Jilin University, Changchun, China

Tóm tắt

AbstractKaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti‐inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging‐related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE‐treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency‐related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE‐treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function.

Từ khóa


Tài liệu tham khảo

10.1089/rej.2006.0516

10.1016/j.cell.2005.08.020

10.1016/S0955-2863(02)00255-3

10.1016/S0092-8674(03)00392-1

10.1016/j.foodchem.2012.11.139

10.1007/s12020-018-1525-4

10.1016/j.fct.2011.04.031

10.1155/2012/646354

Dai X., 2017, Melatonin improves the fertilization ability of post‐ovulatory aged mouse oocytes by stabilizing ovastacin and Juno to promote sperm binding and fusion, Human Reproduction, 32, 598

Diaz H., 2004, Postovulatory ageing induces structural changes in the mouse zona pellucida, Journal of Submicroscopic Cytology and Pathology, 36, 211

10.1038/35041687

10.1016/S1534-5807(01)00055-7

10.1016/j.cell.2016.01.047

10.1079/9781780646022.0000

10.1016/j.ejphar.2015.05.056

10.1080/01635589309514267

10.1016/j.taap.2009.02.014

10.1111/j.1474-9726.2009.00539.x

10.1007/s00204-007-0215-4

10.4161/cbt.7.7.6164

10.1080/08923970701812530

10.1016/j.devcel.2005.03.006

10.1186/s12906-016-1354-z

Lee Y. J., 2010, Kaempferol protects HIT‐T15 pancreatic beta cells from 2‐deoxy‐D‐ribose‐induced oxidative damage, Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 24, 419, 10.1002/ptr.2983

10.1006/meth.2001.1262

Lord T., 2015, The role of reactive oxygen species and oxidative stress in post‐ovulatory ageing and apoptosis of the mammalian oocyte

10.1095/biolreprod.112.106450

10.1016/j.cell.2010.09.013

10.1111/asj.12360

10.1530/REP-07-0021

10.1074/jbc.M309660200

10.1093/humupd/dmp014

10.1096/fj.201700908R

Middleton E., 2000, The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer, Pharmacological Reviews, 52, 673

10.1038/srep28889

10.1006/dbio.1999.9344

10.1016/j.taap.2005.04.004

10.1007/s10495-007-0756-2

10.1089/jmf.2008.0006

10.1021/np9904509

10.1016/0891-5849(95)02227-9

10.1016/j.ejphar.2005.10.061

10.1089/ars.2007.9.49

10.1159/000182754

10.1016/j.freeradbiomed.2004.04.002

10.1016/j.fct.2014.07.038

10.1016/j.stem.2008.10.004

10.1073/pnas.1718833115

10.3109/10715762.2010.507670

10.1095/biolreprod61.5.1347

10.1093/humupd/6.6.532

10.1093/molehr/4.3.281

10.1016/j.neulet.2008.03.062

10.1002/sca.21312

10.18632/oncotarget.16219

10.1016/j.immuni.2015.02.002

10.1093/humrep/16.5.909

10.1111/ajco.12173

10.1262/jrd.2018-031

10.1262/jrd.20001

10.18632/aging.100911

10.1016/j.jss.2008.02.036

10.1111/asj.12611

Thông tin
Thông tin xuất bản

Animal Science Journal

Tập 90 Số 11

1417-1425

Thông tin tác giả