Joint effects of microwave and chromium trioxide on root tip cells of Vicia faba
Tóm tắt
The mutagenic effects of microwave and chromium trioxide (CrO3) on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO3. The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO3, in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO3 concentration. We concluded that microwave and CrO3 had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells.
Tài liệu tham khảo
Forgacs, Z., Kubinyi, G., Sinay, G., 2005. Effects of 1800 MHz GSM-like exposure on the gonadal function and hematological parameters of male mice. Magy. Onkol., 49(2):149–151.
Garcia-Rodriguez, M.C., Lopez-Santiago, V., Altamirano-Lozano, M., 2001. Effect of chlorophyllin on chromium trioxide-induced micronuclei in polychromatic erythrocytes in mouse peripheral blood. Mutat. Res., 496(1–2):145–151.
Kim, H.Y., Lee, S.B., Jang, B.C., 2004. Subchronic inhalation toxicity of soluble hexavalent chromium trioxide in rats. Arch. Toxicol., 78(7):363–368. [doi:10.1007/s00204-004-0553-4]
Knasmuller, S., Gottmann, E., Steinkellner, H., Fomin, A., Pcickl, C., Paschke, A., God, R., Kundi, M., 1998. Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays. Mutat. Res., 420(1–3):37–48.
Lacy-Hulbert, A., Metcalfe, J.C., Hesketh, R., 1998. Biological responses to electromagnetic field. Faseb. J., 12(6):395–420.
Litviakov, N.V., Buldakov, M.A., Cherdyntseva, N.V., Rostov, V.V., Klimov, A.I., Bol’shakov, M.A., 2005. Effect of impulse-intermittent ultrahigh frequency irradiation on synthesis of nucleic acids in tumor cells. Radiats. Biol. Radioecol., 45(4):460–463.
Ozaki, T., Tabuse, K., Tsuji, T., Nakamura, Y., Kakudo, K., Mori, I., 2003. Microwave cell death: enzyme histochemical evaluation for metastatic carcinoma of the liver. Pathol. Int., 53(12):837–845. [doi:10.1046/j.1440-1827.2003.01571.x]
Pavicic, I., 2004. Impact of radiofrequency/microwave radiation on cell and cytoskeleton structure. Arh. Hig. Rada. Toksikol., 55(4):321–328.
Qian, X.W., 2004a. Mutagenic effects of chromium trioxide on root tip cells of Vicia faba. Journal of Zhejiang University (SCIENCE), 5(12):1570–1576 (in Chinese). [doi:10.1631/jzus.2004.1570]
Qian, X.W., 2004b. Study on teratogenic effect of potassium dichromate on Vicia faba root tip cells. Hereditas, 26(3):337–342 (in Chinese).
Sher, L., 2000. The effects of natural and man-made electromagnetic fields on mood and behavior: the role of sleep disturbances. Med. Hypotheses, 54(4):630–633. [doi:10.1054/mehy.1999.0912]
Sripakdee, D., Sukontason, K.L., Piangjai, S., Ngern-klun, R., Sukontason, K., 2005. Effect of microwave irradiation on the blow fly Chrysomya megacephala (F.) (Diptera: Calliphoridae). Southeast Asian J. Trop. Med. Public Heslth., 36(4):893–895.
Szelag, A., Magdalan, J., Kopacz, M., 2003. Assessment of efficacy of quercetin-5′-sulfonic acid sodium salt in the treatment of acute chromium poisoning: experimental studies. Pol. J. Pharmacol., 55(6):1097–1103.