Isozyme studies of genetic diversity and evolution in Hippophae
Tóm tắt
To provide information on the genetic variation, differentiation and evolution in Hippophae seed samples of 25 populations from China, Finland and Russia were electrophoretically analyzed. Of six loci investigated, four were good genetic markers for identifying species and subspecies. The percentages of polymorphic loci per population were 40.3% and 62.5% at 0.95 and 0.99 polymorphic criteria respectively. The mean number of alleles per locus per population was 2.1. Total genetic diversity in the material was 0.4614. Genetic diversity partitioning showed that there was a large amount of diversity residing within geographical populations (0.1354), between subspecies within species (0.1046) and between species (0.2566) but not between geographical populations (0.0114). There were nearly twice as many negative fixation indices as positive ones in Hippophae populations. The phylogenetic tree agreed very well with botanic classifications of the species and subspecies and their geographical distributions, and quantitatively presented the genetic relationships of 25 populations. A detailed view of the evolutionary stages in Hippophae showed clearly a general decline of similarity as evolutionary divergence continued, which further explained the evolution process in Hippophae.
Tài liệu tham khảo
Baker, D.D. & C.R. Schwintzer, 1990. Introduction. In: C.R. Schwintzer & J.D. Tjepkema (Eds.), The Biology of Frankia and Actinorhizal Plants, p.p. 1–13, Academic Press, San Diego & London.
Conkle, M.T., P.D. Hodgskiss, L.B. Nunnally & S.C. Hunter, 1982. Starch gel electrophoresis of conifer seeds: a laboratory manual. Pacific Southwest Forest and Range Experiment Station, Berkeley, California.
Hamrick, J.L. & M.J.W. Godt, 1990. Allozyme diversity in plant species. In: A.H.D. Brown, M.T. Clegg, A.L. Kahler & B.S. Wier (Eds.), Plant Population Genetics, Breeding, and Genetic Resources, p.p. 43–63, Sinauer, Massachusetts.
Hedrick, P.W., 1985. Genetics of Populations. Jones and Bartlett Publishers, Boston.
Huang, Q. & Y. Tong, 1992. Research on the variations of major economic traits of Subsp. sinensis Rousi. Hippophae 2: 18–21 (in Chinese).
Kephart, S., 1990. Starch gel electrophoresis of plant isozymes: a comparative analysis of techniques. Amer. J. Bot. 77: 693–712.
Koul, P. & R. Bhargava, 1986. Association of isoperoxidases with microspore differentiation in plants. Phytomorphology 36: 117–120.
Lian, Y., 1988. New discovery in the genus Hippophae. Acta Phytotaxonomica Sinica 26: 235–237 (in Chinese, abstract in English).
Liu, S.W. & T.N. He, 1988. The genus Hippophae from Qing-Zang Plateau. Acta Phytotaxonomica Sinica 16: 106–107 (in Chinese).
Lu, R., M. Mo, S. Chen & Y. Cao, 1989. Analysis of peroxidase isozyme in the sea buckthorn (Hippophae) of China. Proc. of international symposium on Sea buckthorn, p.p. 138–144.
Maestri, E., F.M. Restivo, G.P. Marziani-Long, A. Falavigna & F. Tassi, 1991. Isozyme gene marker in the dioecious species Asparagus officinalis. Theo. Appl. Gene. 81: 613–618.
Muona, O., R. Yazdani & G. Lindqvist, 1987. Analysis of linkage in Picea abies. Hereditas 106: 31–36.
Nei, M., 1972. Genetic distance between populations. American Naturalist 106: 283–292.
Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA 70: 3321–3323.
Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.
Parthasarathi, H. & V.G. Angadi, 1984. Peroxidase isoenzyme pattern in the living bark tissue as an index of male and female identity in dioecious Bursera penicillata. Current Science of India 53: 533–534.
Pasteur, N., G. Pasteur, F. Bonhomme, J. Catalan & J. Britton-Davidian, 1988. Practical Isozyme Genetics. Ellis Horwood, Chichester.
Rousi, A., 1971. The genus Hippophae L.: A taxonomic study. Annales Botanici Fennici 8: 177–227.
Schnabel, A. & J.L. Hamrick, 1990. Nonrandom associations between sex and 6-phosphogluconate dehydrogenase isozyme genotypes in Gleditsia triacanthos L. J. Hered. 81: 230–233.
Suganuma, H. & F. Iwasaki, 1983. Sex identification of dioecious plants by isozyme method: data (Phoenix dactylifera L.). Japanese Journal of Tropical Agriculture (in Japanese, summary in English) 27: 75–78.
Swofford, D.L. & R.B. Selander, 1989. Biosys-1: A Fortran program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J. Hered. 72: 281–283.
Vallejos, C.E., 1983. Enzyme activity staining. In: T. Tanksley, & J. Orton (Eds.), Isozymms in plant genetics and breeding, Part A, p.p. 496–515. Elsevier Science Publishers, Amsterdam.
Zhang, G., 1988. A study on the germplasm resources and superior tree of Hippophae. Hippophae 2: 10–15 (in Chinese).