Isolation of three new metabolites and intervention of diazomethane led to separation of compound 1 & 2 from an endophytic fungus, Cryptosporiopsis sp. depicting cytotoxic activity

Springer Science and Business Media LLC - Tập 26 - Trang 2900-2908 - 2017
Sunil Kumar1,2, Yedukondalu Nalli1,2, Masroor Qadri2,3, Syed Riyaz-Ul-Hassan2,3, Naresh K. Satti1, Vivek Gupta4, Shashi Bhushan5, Asif Ali1,2
1Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu- Tawi, India
2Academy of Scientific and Innovative Research, CSIR-Indian Institute of Medicine, Jammu-Tawi, India
3Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Jammu-Tawi, India
4Post-Graduate Department of Physics & Electronics, University of Jammu, Jammu Tawi, India
5Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India

Tóm tắt

The discovery of three new natural products (1, 4, and 5), one semi-synthetic derivative (3) along with two known compounds (2 and 6) were isolated from an endophytic fungus Cryptosporiopsis sp. The structural elucidations of 1–6 were authenticated by one-dimensional and two-dimensional nuclear magnetic resonance, mass spectroscopy, and X-ray diffraction analysis. Herein, we intervention of diazomethane as tool that help in the crystallization and isolation of inseparable mixtures of compounds 1 and 2. Compounds (1–6) were screened for cytotoxic activity against six cancer cell lines in which the 4-epi-ethisolide (2) exhibited moderate activity with IC50 values 11 µM in HL-60, whereas the compound 3 lost its cytotoxic potentiality, but it displayed moderate antimicrobial activity. The result illustrates that the methylene moiety in 2 plays significant role in cytotoxic potential.

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