Isolation of nucleic acids from microfungi by treatment with thioglycolic acid
Tóm tắt
A method for the isolation of nucleic acids from various microfungi by sensitization to the lysis with thioglycolic acid is described. Cells, grown with strong aeration, are suddenly suspended in buffered NaCl-ethylenediaminetetraacetate (EDTA) solution pH 8,5, in the presence of thioglycolic acid for 3—5 hours at 50°C. The method proved to give high per cent of broken cells together with good recovery of nucleic acids.
Tài liệu tham khảo
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