Isolation of a polymorphic genomic clone from chromosome 7

Springer Science and Business Media LLC - Tập 77 - Trang 122-126 - 1987
Kevin A. Davies1, Laszlo Lorand2, Michael Waterfield3, Brandon Wainwright1, Martin Farrall1, Robert Williamson1
1Department of Biochemistry, St. Mary's Hospital Medical School, London, UK
2Department of Biochemistry, Molecular and Cell Biology, North Western University, Chicago, USA
3Ludwig Institute for Cancer Research, London, UK

Tóm tắt

A peptide prepared from purified factor 13B (F13B) was sequenced, and a single, long oligonucleotide corresponding to its cognate DNA sequence was constructed and used to screen a chromosome 7 specific genomic library. The positive clone isolated, designated pKV13, was only related to F13B at the oligonucleotide region, but has proved to be a valuable chromosome 7 marker. pKV13 maps to 7pter-q22 in hybrid cell lines, and is present in a chromosome-mediated gene transfer (CMGT) cell line that also contains met and other 7q probes. pKV13 defines a common MspI restriction fragment length polymorphism (RFLP), and is genetically linked to two markers on the long arm of chromosome 7, B79a and COL1A2, both themselves linked to the cystic fibrosis locus. Multipoint linkage analysis demonstrates that KV13 maps centromeric to both B79a and COLIA2. pKV13 has been used to demonstrate the existence of rearrangements within CMGT hybrisd, and will also prove valuable in multipoint linkage studies of other 7q markers. Finally, pKV13 provides a new polymorphic locus for the characterisation of 7q deletions in myeloid disorders such as myelodysplastic syndrome.

Tài liệu tham khảo

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