Isolation of a novelAgrobacteriun spp capable of degrading a range of nitrile compounds
Tóm tắt
Using soil enrichment techniques we have isolated micro-organisms capable of degrading simple nitrile compounds. One species identified as anAgrobacterium spp. was examined in detail. This isolate was capable of utilising a range of aliphatic and aromatic nitriles as sole sources of carbon and nitrogen. Assays for enzymes involved in nitrile degradation and growth/production studies with this organism growing on acetonitrile indicated that breakdown occurred via a two step mechanism firstly to the amide and then via the production of the corresponding acid with the subsequent release of ammonia. Our studies indicate that the system of nitrile breakdown is inducible and levels of the amidase are 170 times that of the nitrile hydratase in crude extracts.
Tài liệu tham khảo
Aislabie, J. and Atlas, R.M. (1988). Appl. Envir. Micro. 54(9) 2197–2202.
Asano, Y., Tani, Y., and Yamada, H. (1980). Agric. Biol. Chem. 46(5) 2251–2252.
Harris, E.L.V., and Angal, S. (1989). Protein purification methods. Oxford Uni Press.
Harper, D.B. (1977). Biochem. J. 165 309–319.
Harper, D.B. (1985). Int. J. Biochem. 17(6) 677–683.
Heaney, R.K., and Fenwick, G.R. (1987). in ‘Natural toxicants in food’ Ellis Horwood.
Ingvorsen, K. et al (1988). in ‘Cyanide compounds in Biology’. Wiley (Ciba Symp 140).
Jallageas, J.C., Arnaud, A., and Galzy, P. (1978). J. Chromatography 166 181–187.
Jallageas, J.C., Arnaud, A., and Galzy, P. (1980). in ‘Advances in Bioengineering’ Springer.
Kato, H., and Yamamura, K. (1976). US Patent No. 3,940,332.
Lee, Y.H., and Chang, H.N. (1989). Biotech Letts. 11(1) 23–26.
Mark, H.F. et al (1984). Encyclopedia of Chemical Technology. Wiley.
Nawaz, M.S. et al (1989). Appl. Envir. Micro 55 2267–2274.
Robinson, W.G. and Hook, R.H. (1964). J. Biol. Chem. 239(12) 4257–4267.
Silver, E.H. et al. (1982). Drug Metabolism and Disposition. 10(5) 495–498.
Vaughan, P.A., Knowles, C.J. and Cheetham, P.S. (1989). Enz. Micro. Tech 11 815.
Yamamoto, K. et al. (1990). Appl. Envir. Micro. 56(10) 3125–3129.