Isolation of a new restriction enzyme,ApaCI, an isoschizomer ofBamHI produced byAcetobacter pasteurianus
Tóm tắt
A new Type II restriction endonucleaseApaCI purified fromAcetobacter pasteurianus is an isoschizomer ofBamHI that cleaves at the nucleotide sequence 5′-G/GATCC-3′ of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30 000 units of cleavage activity from 10 g of freshly harvested cells. The resultingApaCI preparation is free of contaminant nuclease activities that might interfere within vitro manipulation of DNA.
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