Isolation of a Chromosomal Region ofKlebsiella pneumoniaeAssociated with Allantoin Metabolism and Liver Infection

Infection and Immunity - Tập 72 Số 7 - Trang 3783-3792 - 2004
Huei‐Chi Chou1, Cha‐Ze Lee2, Li‐Chen Ma3, Chi‐Tai Fang2, Shan‐Chwen Chang2, Jann‐Tay Wang2,3
1Department of Microbiology, National Taiwan University College of Medicine, 1 Jen-Ai Road, Taipei, Taiwan.
2Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
3Department of Microbiology, National Taiwan University College of Medicine

Tóm tắt

ABSTRACTKlebsiella pneumoniaeliver abscess with metastatic complications is an emerging infectious disease in Taiwan. To identify genes associated with liver infection, we used a DNA microarray to compare the transcriptional profiles of three strains causing liver abscess and three strains not associated with liver infection. There were 13 clones that showed higher RNA expression levels in the three liver infection strains, and 3 of these 13 clones contained a region that was absent in MGH 78578. Sequencing of the clones revealed the replacement of 149 bp of MGH 78578 with a 21,745-bp fragment in a liver infection strain, NTUH-K2044. This 21,745-bp fragment contained 19 open reading frames, 14 of which were proven to be associated with allantoin metabolism. The K2044 (ΔallS) mutant showed a significant decrease of virulence in intragastric inoculation of BALB/c mice, and the prevalence of this chromosomal region was significantly higher in strains associated with liver abscess than in those that were not (19 or 32 versus 2 of 94;P= 0.0001 [χ2test]). Therefore, the 22-kb region may play a role inK. pneumoniaeliver infection and serve as a marker for rapid identification.

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Thông tin xuất bản

Infection and Immunity

Tập 72 Số 7

3783-3792

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