Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

Polymers - Tập 13 Số 23 - Trang 4257
Sol Lee Park1, Jang Yeon Cho, Su Hyun Kim1, Shashi Kant Bhatia1,2, Ranjit Gurav1, See‐Hyoung Park3, Kyungmoon Park1, Yung‐Hun Yang1,3,2
1Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea;
2Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, Korea.
3Department of Biological and Chemical Engineering, Hongik University, Sejong, 30016, Korea

Tóm tắt

Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, Microbulbifer sp. SOL66 (94.18% 16S rRNA with similarity to Microbulbifer hydrolyticus) demonstrated the highest degradation activity among five newly screened Microbulbifer genus strains. Microbulbifer sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 °C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that Microbulbifer sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date.

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Polymers

Tập 13 Số 23

4257

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