Isolation, characterization, and expression analysis of a class IV chitin synthase gene from the edible basidiomycetous mushroom Pleurotus ostreatus

Mycoscience - Tập 48 - Trang 176-181 - 2007
Mikihiro Nishihara1, Akira Watanabe1, Yasuhiko Asada1
1Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kagawa, Japan

Tóm tắt

We isolated and characterized the class IV chitin synthase gene (Pochs1) from the edible basidiomycetous mushroom, Pleurotus ostreatus. Real-time quantitative RT-PCR analysis of the transcriptional expression pattern of Pochs1 in the course of fruit-body formation of P. ostreatus revealed that the transcriptional level of Pochs1 is higher in the stage of immature fruit body than in the stages of mycelia and mature fruit body. Furthermore, the transcriptional level of Pochs1 in mycelia was shown to have increased significantly by the temperature-downshift treatment, which is one of the important factors for inducing the onset of fruit-body formation of P. ostreatus.

Tài liệu tham khảo

S Bartnicki-García (1968) ArticleTitleCell wall, chemistry, morphogenesis and taxonomy of fungi Annu Rev Microbiol 22 87–108 Occurrence Handle4879523 Occurrence Handle10.1146/annurev.mi.22.100168.000511 AR Bowen JL Chen-Wu M Momany R Young PJ Szaniszlo PW Robbins (1992) ArticleTitleClassification of fungal chitin synthases Proc Natl Acad Sci U S A 89 519–523 Occurrence Handle1731323 Occurrence Handle10.1073/pnas.89.2.519 Occurrence Handle1:CAS:528:DyaK3sXhs1Cjt7o%3D CE Bulawa (1993) ArticleTitleGenetics and molecular biology of chitin synthesis in fungi Annu Rev Microbiol 47 505–534 Occurrence Handle8257107 Occurrence Handle10.1146/annurev.mi.47.100193.002445 Occurrence Handle1:CAS:528:DyaK3sXms1Kjtr0%3D J Chen-Wu J Zwicher AR Bowen PW Robbins (1992) ArticleTitleExpression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans Mol Microbiol 6 497–502 Occurrence Handle1560778 Occurrence Handle10.1111/j.1365-2958.1992.tb01494.x Occurrence Handle1:CAS:528:DyaK3sXhs1ajtLs%3D Y Chigira K Abe K Gomi T Nakajima (2002) ArticleTitle chsZ, a gene for a novel class of chitin synthase from Aspergillus oryzae Curr Genet 41 261–267 Occurrence Handle12172967 Occurrence Handle10.1007/s00294-002-0305-z Occurrence Handle1:CAS:528:DC%2BD38Xls12mur0%3D WJ Choi B Santos A Durán E Cabib (1994) ArticleTitleAre yeast chitin synthases regulated at the transcriptional or the posttranslational level? Mol Cell Biol 14 7685–7694 Occurrence Handle7969112 Occurrence Handle1:CAS:528:DyaK2MXisVWgsLw%3D A Durán B Bowers E Cabib (1975) ArticleTitleChitin synthetase zymogen is attached to the yeast plasma membrane Proc Natl Acad Sci U S A 72 3952–3955 Occurrence Handle1105572 Occurrence Handle10.1073/pnas.72.10.3952 M Ichinomiya T Motoyama M Fujiwara M Takagi H Horiuchi A Ohta (2002) ArticleTitleRepression of chsB expression reveals the functional importance of class IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulans Microbiology 148 1335–1347 Occurrence Handle11988507 Occurrence Handle1:CAS:528:DC%2BD38XktFShsb4%3D VI Klimyuk BJ Carroll CM Thomas JD Jones (1993) ArticleTitleAlkali treatment for rapid preparation of plant material for reliable PCR analysis Plant J 3 493–494 Occurrence Handle8220456 Occurrence Handle10.1046/j.1365-313X.1993.t01-26-00999.x Occurrence Handle1:CAS:528:DyaK3sXkvVOns7Y%3D MR Lerner JA Boyle SM Mount SL Wolin JA Steitz (1980) ArticleTitleAre snRNPs involved in splicing? Nature (Lond) 283 220–224 Occurrence Handle10.1038/283220a0 Occurrence Handle1:CAS:528:DyaL3cXhsF2jtr8%3D YG Liu RF Whittier (1995) ArticleTitleThermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking Genomics 25 674–681 Occurrence Handle7759102 Occurrence Handle10.1016/0888-7543(95)80010-J Occurrence Handle1:CAS:528:DyaK2MXktFOqsr4%3D T Motoyama M Sudoh H Horiuchi A Ohta M Takagi (1994) ArticleTitleIsolation and characterization of two chitin synthase genes of Rhizopus oligosporus Biosci Biotechnol Biochem 58 1685–1693 Occurrence Handle7765484 Occurrence Handle1:CAS:528:DyaK2cXmvFCiurc%3D Occurrence Handle10.1271/bbb.58.1685 C Müller CM Hjort K Hansen J Nielsen (2002) ArticleTitleAltering the expression of two chitin synthase genes differentially affects the growth and morphology of Aspergillus oryzae Microbiology 148 4025–4033 Occurrence Handle12480906 CA Munro DA Schofield GW Gooday NAR Gow (1998) ArticleTitleRegulation of chitin synthesis during dimorphic growth of Candida albicans Microbiology 144 391–401 Occurrence Handle9493376 Occurrence Handle1:CAS:528:DyaK1cXhtlantb4%3D Occurrence Handle10.1099/00221287-144-2-391 S Nagahashi M Sudoh N Ono R Sawada E Yamaguchi Y Uchida T Mio M Takagi M Arisawa H Yamada-Okabe (1995) ArticleTitleCharacterization of chitin synthase 2 of Saccharomyces cerevisiae: implication of two highly conserved domains as possible catalytic sites J Biol Chem 270 13961–13967 Occurrence Handle7775457 Occurrence Handle10.1074/jbc.270.23.13961 Occurrence Handle1:CAS:528:DyaK2MXmt1yqtr8%3D GA Niño-Vega L Carrero G San-Blas (2004) ArticleTitleIsolation of the CHS4 gene of Paracoccidioides brasiliensis and its accommodation in a new class of chitin synthases Med Mycol 42 51–57 Occurrence Handle14982114 Occurrence Handle10.1080/1369378031000153811 Occurrence Handle1:CAS:528:DC%2BD2cXhvFeitro%3D M Pammer P Briza A Ellinger T Schuster R Stucka H Feldmann M Breitenbach (1992) ArticleTitle DIT101 (CSD2, CAL1), a cell cycle-regulated yeast gene required for synthesis of chitin in cell walls and chitosan in spore walls Yeast 8 1089–1099 Occurrence Handle1293886 Occurrence Handle10.1002/yea.320081211 Occurrence Handle1:CAS:528:DyaK3sXpsFCitg%3D%3D C Roncero (2002) ArticleTitleThe genetic complexity of chitin synthesis in fungi Curr Genet 41 367–378 Occurrence Handle12228806 Occurrence Handle10.1007/s00294-002-0318-7 Occurrence Handle1:CAS:528:DC%2BD38XntVKjs7c%3D J Ruiz-Herrera JM González-Prieto R Ruiz-Medrano (2002) ArticleTitleEvolution and phylogenetic relationships of chitin synthases from yeasts and fungi FEMS Yeast Res 1 247–256 Occurrence Handle12702327 Occurrence Handle10.1111/j.1567-1364.2002.tb00042.x Occurrence Handle1:CAS:528:DC%2BD38XivVyrur0%3D H Sakai S Kajiwara (2003) ArticleTitleA stearoyl-CoA-specific Δ9 fatty acid desaturase from the basidiomycetes Lentinula edodes Biosci Biotechnol Biochem 67 2431–2437 Occurrence Handle14646204 Occurrence Handle10.1271/bbb.67.2431 Occurrence Handle1:CAS:528:DC%2BD3sXps1elsr8%3D CA Specht Y Liu PW Robbins CE Bulawa N Iartechouk KR Winter PJ Riggle JC Rhodes CL Dodge DW Culp PT Borgia (1996) ArticleTitleThe chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis Fungal Genet Biol 20 153–167 Occurrence Handle8810520 Occurrence Handle10.1006/fgbi.1996.0030 Occurrence Handle1:CAS:528:DyaK28XlvFKlt7w%3D S Sreenivasaprasad KS Burton DA Wood (2000) ArticleTitleCloning and characterization of a chitin synthase gene cDNA from the cultivated mushroom Agaricus bisporus and its expression during morphogenesis FEMS Microbiol Lett 189 73–77 Occurrence Handle10913868 Occurrence Handle1:CAS:528:DC%2BD3cXltVOmu78%3D M Sudoh S Nagahashi M Doi A Ohta M Takagi M Arisawa (1993) ArticleTitleCloning of the chitin synthase 3 gene from Candida albicans and its expression during yeast-hyphal transition Mol Gen Genet 241 351–358 Occurrence Handle8246889 Occurrence Handle10.1007/BF00284688 Occurrence Handle1:CAS:528:DyaK2cXkslCqsLg%3D N Takeshita A Ohta H Horiuchi (2002) ArticleTitle csmA, a gene encoding a class V chitin synthase with a myosin motor-like domain of Aspergillus nidulans, is translated as a single polypeptide and regulated in response to osmotic conditions Biochem Biophys Res Commun 298 103–109 Occurrence Handle12379226 Occurrence Handle10.1016/S0006-291X(02)02418-X Occurrence Handle1:CAS:528:DC%2BD38Xns1Gmur8%3D N Takeshita A Ohta H Horiuchi (2005) ArticleTitleCsmA, class V chitin synthase with a myosin motor-like domain, is localized through direct interaction with actin cytoskeleton in Aspergillus nidulans Mol Biol Cell 16 1961–1970 Occurrence Handle15703213 Occurrence Handle10.1091/mbc.E04-09-0761 Occurrence Handle1:CAS:528:DC%2BD2MXjt1Kqtbg%3D N Takeshita S Yamashita A Ohta H Horiuchi (2006) ArticleTitle Aspergillus nidulans class V and VI chitin synthases CsmA and CsmB, each with a myosin motor-like domain, perform compensatory functions that are essential for hyphal tip growth Mol Microbiol 59 1380–1394 Occurrence Handle16468983 Occurrence Handle10.1111/j.1365-2958.2006.05030.x Occurrence Handle1:CAS:528:DC%2BD28Xit1Shsb4%3D Z Wang PJ Szaniszlo (2000) ArticleTitle WdCHS3, a gene that encodes a class III chitin synthase in Wangiella (Exophiala) dermatitidis, is expressed differentially under stress conditions J Bacteriol 182 874–881 Occurrence Handle10648509 Occurrence Handle10.1128/JB.182.4.874-881.2000 Occurrence Handle1:CAS:528:DC%2BD3cXhtVWqsb8%3D Q Wang H Liu PJ Szaniszlo (2002) ArticleTitleCompensatory expression of five chitin synthase genes, a response to stress stimuli, in Wangiella (Exophiala) dermatitidis, a melanized fungal pathogen of humans Microbiology 148 2811–2817 Occurrence Handle12213927 Occurrence Handle1:CAS:528:DC%2BD38Xnt1elu74%3D B Xoconostle-Cázares CA Specht PW Robbins Y Liu C León J Ruiz-Herrera (1997) ArticleTitle Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis Fungal Genet Biol 22 199–208 Occurrence Handle9454647 Occurrence Handle10.1006/fgbi.1997.1014 T Yabe T Yamada-Okabe T Nakajima M Sudoh M Arisawa (1998) ArticleTitleMutational analysis of chitin synthase 2 of Saccharomyces cerevisiae Eur J Biochem 258 941–947 Occurrence Handle9990311 Occurrence Handle10.1046/j.1432-1327.1998.2580941.x Occurrence Handle1:CAS:528:DyaK1MXisFeitQ%3D%3D T Yamazaki T Kiyofuji T Johjima S Kajiwara A Tsukamoto J Sugiura K Shishido (2004) ArticleTitleIsolation of a ras gene from the basidiomycete Coriolus hirsutus and use of its promoter for the expression of Pleurotus ostreatus manganese (II) peroxidase cDNA in C. hirsutus Mycoscience 45 317–323 Occurrence Handle10.1007/s10267-004-0191-y Occurrence Handle1:CAS:528:DC%2BD2MXpsFaitw%3D%3D