Isolation and primary culture of chicken intestinal epithelial cells retaining normal in vivo-like morphology

Methods in Cell Science - Tập 15 Số 1 - Trang 15-18 - 1993
David J. Caldwell1, Robert E. Droleskey2, Marcel H. Elissalde3, Michael H. Kogut2, John R. DeLoach2, Billy M. Hargis1
1Departments of Veterinary Pathobiology and Poultry Science, Texas Agricultural Experiment Station, Texas A&M University System, USA
2United States Department of Agriculture-Agriculture Research Service, Food Animal Protection Research Laboratory, Texas
3Department of Microbiology, University of Arkansas, USA

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Droleskey, R. E.; Caldwell, D. J., Scanlan, C. M., et al.In vitro binding ofSalmonella to isolated chicken enterocytes. Poult. Sci. 70(suppl. 1):3; 1991.

Elissalde, M. H.; Stipanovic, R. D., Norman, J. O., et al. Development of a heliothis cell culture bioassay for accessing the toxicity of methyl parathion, permethrin, and limonene. Proceedings of the Beltwide Cotton Production Research Conferences. Memphis, TN: National Cotton Council of America; 1988:352–353.

Evans, E. M.; Wrigglesworth, J. M., Burdett, K., et al. Studies on epithelial cells isolated from guinea pig small intestine. J. Cell Biol. 51:452–464; 1971.

Holt, P. S.; Buckley, S.; DeLoach, J. R. Detection of the lethal effects of T-2 mycotoxins on cells using a rapid colorimetric viability assay. Toxicol. Lett. 39:301–312; 1987.

Holt, P. S.; Buckley, S.; Norman, J. O., et al. Cytotoxic effect of T-2 mycotoxin on cells in culture as determined by a rapid colorimetric bioassay. Toxicon 26:549–558; 1988.

Kimmich, G. Preparation and properties of mucosal epithelial cells isolated from small intestine of the chicken. Biochemistry 9:3659–3668; 1970.

Koo, F. W.; Peterson, J. W., Houston, C. W., et al. Pathogenesis of experimental Salmonellosis: inhibition of protein synthesis by cytotoxin. Infect. Immun. 43:93–100; 1984.