Isolation and development of 12 polymorphic tetranucleotide microsatellite markers for the eastern hellbender (Cryptobranchus alleganiensis alleganiensis)
Tóm tắt
In order to analyze population genetic structure at multiple spatial scales, 12 polymorphic microsatellite loci were developed for the eastern hellbender (Cryptobranchus alleganiensis alleganiensis). The number of alleles per locus ranged from 6 to 12 (mean of 9.25 per locus) and the observed heterozygosity ranged from 0.645 to 0.935 among 31 individuals. Two loci exhibited significant deviations from Hardy–Weinberg equilibrium. No evidence for linkage disequilibrium was detected between pairs of loci. These markers will be used to examine population genetic structure across the geographic range of this declining species.
Tài liệu tham khảo
Ayres K, Overall AD (2004) API-CALC 1.0: a computer program for calculating the average probability of identity allowing for substructure, inbreeding, and the presence of close relatives. Mol Ecol Notes 4:315–318
Beheler AS, Fike JA, Dharmarajan G, Rhodes OE, Serfass TL (2004) Ten new polymorphic microsatellite loci for North American river otters (Lontra canadensis) and their utility in related mustelids. Mol Ecol 5:602–604
Hamilton MB, Pincus EL, DiFiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507
Jehle R, Arntzen JW (2002) Review: microsatellite markers in amphibian conservation genetics. Herpetol J 12:1–9
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106
Mayasich J, Grandmaison D, Phillips C (2003) Eastern hellbenders status assessment report. Natural Resources Research Institute, Technical Report 9:1-41 + ii + appendices
Nickerson MA, Krysko KL, Owen RD (2002) Ecological status of the hellbender and the mudpuppy salamanders in the Great Smokey Mountains National Park. JNCAS 118:27–34
Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Resources 4:535–538
Rousset F (2008) GENEPOP’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resources 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Stuart SN, Chansen JS, Cox NA, Young BE, Rodriques ASL, Fischman DL, Waller RW (2004) Status and trends of amphibian declines and extinctions worldwide. Science 306:1783–1786
Wheeler BA, Prosen E, Mathis A, Wilkinson RF (2003) A case of amphibian decline in a long-lived salamander: a long term study of hellbender populations. Biol Conserv 109:151–156
Williams RN, DeWoody JA (2004) Fluorescent dUTP helps characterize ten novel tetranucleotide microsatellites from an enriched salamander (Ambystoma texanum) genomic library. Mol Ecol Notes 4:17–19