Isolation and characterization of endothelial progenitor cells from Rhesus monkeys
Tóm tắt
Endothelial progenitor cells (EPCs) are increasingly becoming a major focus of regenerative medicine research and practice. The present study was undertaken to establish an appropriate procedure for isolation and characterization of EPCs from Rhesus monkeys for regenerative medicine research. Selective CD34+ and nonselective mononuclear EPCs were isolated from bone marrow and cultured under varying conditions. The results showed that nonselective mononuclear EPCs were a better choice for high yield of the target cells. The cells grew in M 200 better than in EGM-2, and supplementation with fetal bovine serum promoted cell proliferation; but serum level at 7.5% was better than at 10%. In addition, surface coating of the culture dishes with human fibronectin significantly improved the proliferation and ontogeny of the isolated EPCs. Immunocytochemistry including detection of markers CD34, CD133 and CD31 and double-staining for Ac-LDL and lectin verified the purity of the cultured mononuclear EPCs. By a thorough analysis, we established a practical procedure for isolation and propagation of EPCs from Rhesus monkeys. This procedure would help using these valuable cells for regenerative medicine research.
Tài liệu tham khảo
Martinez-Estrada OM, Munoz-Santos Y, Julve J, Reina M, Vilaro S: Human adipose tissue as a source of Flk-1+ cells: new method of differentiation and expansion. Cardiovasc res 2005, 65: 328–333. 10.1016/j.cardiores.2004.11.015
Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S: Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood 2000, 95: 952–958.
Eggermann J, Kliche S, Jarmy G, Hoffmann K, Mayr-Beyrle U, Debatin KM, Waltenberger J, Beltinger C: Endothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood. Cardiovasc Res 2003, 58: 478–486. 10.1016/S0008-6363(03)00252-9
Li HQ, Zhao Q, Zhu D, Liu J, Ye XF: Transplantation of bone marrow-derived endothelial progenitor cells preconditioned with ex vivo 17beta-estradiol enhances healing efficacy after acute myocardial infarction. Zhonghua xin xue guan bing za zhi 2011, 39: 420–423.
Yoon CH, Hur J, Park KW, Kim JH, Lee CS, Oh IY, Kim TY, Cho HJ, Kang HJ, Chae IH, Yang HK, Oh BH, Park YB, Kim HS: Synergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells: the role of angiogenic cytokines and matrix metalloproteinases. Circulation 2005, 112: 1618–1627. 10.1161/CIRCULATIONAHA.104.503433
Kawamoto A, Tkebuchava T, Yamaguchi J, Nishimura H, Yoon YS, Milliken C, Uchida S, Masuo O, Iwaguro H, Ma H, Hanley A, Silver M, Kearney M, Losordo DW, Isner JM, Asahara T: Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia. Circulation 2003, 107: 461–468. 10.1161/01.CIR.0000046450.89986.50
Badorff C, Brandes RP, Popp R, Rupp S, Urbich C, Aicher A, Fleming I, Busse R, Zeiher AM, Dimmeler S: Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes. Circulation 2003, 107: 1024–1032. 10.1161/01.CIR.0000051460.85800.BB
Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T: Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. New Engl j med 2003, 348: 593–600. 10.1056/NEJMoa022287
Aoki J, Serruys PW, van Beusekom H, Ong AT, McFadden EP, Sianos G, van der Giessen WJ, Regar E, de Feyter PJ, Davis HR, Rowland S, Kutryk MJ: Endothelial progenitor cell capture by stents coated with antibody against CD34: the HEALING-FIM (Healthy Endothelial Accelerated Lining Inhibits Neointimal Growth-First In Man) Registry. J Am Coll Cardiol 2005, 45: 1574–1579. 10.1016/j.jacc.2005.01.048
Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, Kearney M, Li T, Isner JM, Asahara T: Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci USA 2000, 97: 3422–3427. 10.1073/pnas.97.7.3422
Hur J, Yoon CH, Kim HS, Choi JH, Kang HJ, Hwang KK, Oh BH, Lee MM, Park YB: Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol 2004, 24: 288–293. 10.1161/01.ATV.0000114236.77009.06
Awad O, Dedkov EI, Jiao C, Bloomer S, Tomanek RJ, Schatteman GC: Differential healing activities of CD34+ and CD14+ endothelial cell progenitors. Arterioscler thromb vasc biol 2006, 26: 758–764. 10.1161/01.ATV.0000203513.29227.6f
Friedrich EB, Walenta K, Scharlau J, Nickenig G, Werner N: CD34-/CD133+/VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities. Circ res 2006, 98: e20-e25. 10.1161/01.RES.0000205765.28940.93
Urbich C, Dimmeler S: Risk factors for coronary artery disease, circulating endothelial progenitor cells, and the role of HMG-CoA reductase inhibitors. Kidney int 2005, 67: 1672–1676. 10.1111/j.1523-1755.2005.00261.x
Dome B, Timar J, Dobos J, Meszaros L, Raso E, Paku S, Kenessey I, Ostoros G, Magyar M, Ladanyi A, Bogos K, Tovari J: Identification and clinical significance of circulating endothelial progenitor cells in human non-small cell lung cancer. Cancer res 2006, 66: 7341–7347. 10.1158/0008-5472.CAN-05-4654
Marsboom G, Pokreisz P, Gheysens O, Vermeersch P, Gillijns H, Pellens M, Liu X, Collen D, Janssens S: Sustained endothelial progenitor cell dysfunction after chronic hypoxia-induced pulmonary hypertension. Stem Cells 2008, 26: 1017–1026. 10.1634/stemcells.2007-0562
Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM: Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer. Brit j cancer 2006, 94: 524–531. 10.1038/sj.bjc.6602952
Khoo CP, Valorani MG, Brittan M, Alison MR, Warnes G, Johansson U, Hawa M, Pozzilli P: Characterization of endothelial progenitor cells in the NOD mouse as a source for cell therapies. Diab/metab res rev 2009, 25: 89–93.
Hatzopoulos AK, Folkman J, Vasile E, Eiselen GK, Rosenberg RD: Isolation and characterization of endothelial progenitor cells from mouse embryos. Development 1998, 125: 1457–1468.
Nolan DJ, Ciarrocchi A, Mellick AS, Jaggi JS, Bambino K, Gupta S, Heikamp E, McDevitt MR, Scheinberg DA, Benezra R, Mittal V: Bone marrow-derived endothelial progenitor cells are a major determinant of nascent tumor neovascularization. Genes dev 2007, 21: 1546–1558. 10.1101/gad.436307
Pitchford SC, Furze RC, Jones CP, Wengner AM, Rankin SM: Differential mobilization of subsets of progenitor cells from the bone marrow. Cell stem cell 2009, 4: 62–72. 10.1016/j.stem.2008.10.017
Hackam DG, Redelmeier DA: Translation of research evidence from animals to humans. JAMA 2006, 296: 1731–1732.
Clarkson TB, Prichard RW, Morgan TM, Petrick GS, Klein KP: Remodeling of coronary arteries in human and nonhuman primates. JAMA j Am Med Assoc 1994, 271: 289–294. 10.1001/jama.1994.03510280051032
Tohno Y, Tohno S, Laleva L, Ongkana N, Minami T, Satoh H, Oishi T, Hayashi M, Sinthubua A, Suwannahoy P, Mahakkanukrauh P: Age-related changes of elements in the coronary arteries of monkeys in comparison with those of humans. Biol trace elem res 2008, 125: 141–153. 10.1007/s12011-008-8167-y
Stein A: Decreasing variability in your cell culture. Biotechniques 2007, 43: 228–229. 10.2144/000112561
Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner JM: Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997, 275: 964–967. 10.1126/science.275.5302.964
Fadini GP, Baesso I, Albiero M, Sartore S, Agostini C, Avogaro A: Technical notes on endothelial progenitor cells: ways to escape from the knowledge plateau. Atherosclerosis 2008, 197: 496–503. 10.1016/j.atherosclerosis.2007.12.039
Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, Magner M, Isner JM, Asahara T: Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med 1999, 5: 434–438. 10.1038/7434
Morineau N, Tang XW, Moreau P, Milpied N, Mahe B, Bataille R, Harousseau JL: Lack of benefit of CD34+ cell selected over non-selected peripheral blood stem cell transplantation in multiple myeloma: results of a single center study. Leukemia 2000, 14: 1815–1820. 10.1038/sj.leu.2401883