Isolation and characterization of a flavonoid 3′‐hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida

Plant Journal - Tập 19 Số 4 - Trang 441-451 - 1999
Filippa Brugliera1, Gina Barri‐Rewell2, Timothy A. Holton2, John G. Mason2
1Florigene Ltd, Collingwood, Victoria, Australia.
2Florigene Ltd, 16 Gipps Street, Collingwood, Victoria 3066, Australia

Tóm tắt

SummaryWe have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3′‐hydroxylase (F3′H). The cDNA encodes a 512 amino acid polypeptide with regions of similarity to petunia flavonoid 3′,5′‐hydroxylases (F3′5′H). Both F3′H and F3′5′H are cytochromes P450 and are key enzymes in the flavonoid pathway leading to the production of the coloured anthocyanins. The F3 ′H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant. No or very reduced levels of transcripts are detected in ht1/ht1 lines. This is the first report of isolation of a F3 ′H cDNA clone from any species.

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Tài liệu tham khảo

10.1016/S0022-2836(05)80360-2

10.1007/978-94-017-5294-7_3

10.1007/BF00263858

10.1007/BF00027309

10.1046/j.1365-313X.1994.5010081.x

10.1146/annurev.arplant.49.1.311

10.1007/BF00019155

10.1111/j.1439-0523.1991.tb00474.x

10.1515/znc-1986-1-227

10.1515/znc-1980-9-1004

10.1515/znc-1987-9-1026

Forkmann G., 1981, Genetic control of flavanone 3‐hydroxlase activity and flavonoid 3′‐hydroxylase activity in Antirrhinum majus (snapdragon)., Z. Naturforsch., 36, 411, 10.1515/znc-1981-5-612

10.1016/0031-9422(75)80360-8

10.1007/BF00429466

Gerats A.G.M., 1984, Control of anthocyanin synthesis in Petunia hybrida by multiple allelic series of the genes An1 and., An2. Genetics, 106, 501, 10.1093/genetics/106.3.501

10.1105/tpc.2.11.1121

10.1007/BF00504293

10.1038/366276a0

10.1046/j.1365-313X.1993.04061003.x

10.2307/3870058

10.1126/science.227.4691.1229

10.1046/j.1365-313X.1998.00005.x

10.1128/JB.153.1.163-168.1983

10.1007/BF00393415

10.1093/nar/15.23.9627

10.1016/S0044-328X(78)80261-X

10.1046/j.1365-313X.1994.5010069.x

Lazo G.R., 1991, A DNA transformation‐competent Arabidopsis genomic library in Agrobacterium., Biotechnology, 9, 963, 10.1038/nbt1091-963

Menting J.G.T., 1994, Characterization of flavonoid 3′ 5′ ‐hydroxylases in microsomal membrane fraction of Petunia hybrida flowers., Plant Physiol., 106, 633, 10.1104/pp.106.2.633

10.1006/bbrc.1993.1130

10.1097/00008571-199602000-00002

Nelson D.R., 1988, On the membrane topology of vertebrate cytochrome P‐450 proteins., J. Biol. Chem., 263, 6038, 10.1016/S0021-9258(18)68746-0

10.1073/pnas.85.8.2444

10.1105/tpc.5.11.1497

Sambrook J., 1989, Molecular Cloning: a Laboratory Manual

10.1093/dnares/4.3.215

10.1007/BF00392549

Stafford H.A., 1990, Flavonoid Metabolism

10.1007/BF00304915

10.1515/znc-1982-1-205

10.1007/BF00390812

Tabak A.J.H., 1981, Modification of the B‐ring during flavonoid synthesis in Petunia hybrida: Effect of the hydroxylation gene Hf1 on dihydroflavonol intermediates., Planta, 153, 462, 10.1007/BF00394987

10.1093/oxfordjournals.jbchem.a122393

Thompson J.D., 1994, ClustalW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice., Nucl. Acids Res., 2, 4673, 10.1093/nar/22.22.4673

Van Tunen A.J., 1988, Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light‐regulated and differential expression of flavonoid genes., EMBO J., 7, 1257, 10.1002/j.1460-2075.1988.tb02939.x

Turpen T.H., 1986, Rapid isolation of RNA by a guanidium thiocyanate/cesium chloride gradient method., Biotechniques, 4, 11

10.1007/BF00330509

Wiering H., 1974, Genetics of flower colour in Petunia hybrida., Hort. Genen. Phaenen., 17, 117

10.1007/978-3-662-02387-7_6