Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea

International Journal of Genomics - Tập 2021 - Trang 1-7 - 2021
Yahui Chen1,2,3,4, Xuefeng Peng2,4, Jijie Cui2,4, Hongxia Zhang2,4, Donald L. DeAngelis1,2,3, Zhizhong Song1,5,2,4
1Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, Jiangsu, China
2Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong (Ludong University), Yantai, Shandong, China
3Molecular Testing Laboratory of New Plant Varieties in Southern China of State Forestry Administration, Nanjing, Jiangsu, China
4The Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, China
5Department of Plant Science, University of Cambridge, Cambridge CB2 3EA, UK

Tóm tắt

Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K+ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K+ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K+ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K+ transport and distribution in woody plants.

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International Journal of Genomics

Tập 2021

1-7

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