Investigation on revascularization time and initial damage after transplantation of fresh and cryopreserved ovarian tissue in domestic cats

Cell and Tissue Banking - Tập 21 - Trang 303-312 - 2020
Marcella Motta da Costa1, Liudimila Passos Gonçalves1, Marcelle Santos Lemos2, Aline Ramos Marques Marangon1, Carolina Madeira Lucci1
1Department of Physiological Sciences, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil
2Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil

Tóm tắt

The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remaining four pieces were cryopreserved and, after thawing, one was fixed for cryopreservation control and three were transplanted. Grafts were recovered on days 2 (D2), 4 (D4) and 6 (D6) post-transplantation. Blood vessels were identified by immunohistochemistry and doppler ultrasound. Immunohistochemistry showed that the percentages of total tissue area occupied by blood vessels were similar (P > 0.05) in fresh and cryopreserved tissues. In both cases, blood vessel area was significantly higher (P < 0.05) on D4 and D6 compared to D0. Ultrasound analysis showed vascularization improvement on the periphery of grafts from D2 to D4 and from D4 to D6, both in fresh and cryopreserved tissue samples. Nonetheless, there was a significant decrease (P < 0.05) in the percentage of morphologically normal follicles (MNF) after transplantation compared to non-transplanted tissue (D0), both for fresh and cryopreserved samples. Moreover, the number of follicles found in samples was considerably smaller after grafting. In conclusion, revascularization of ovarian tissue autotransplanted to subcutaneous tissue in domestic cats occurs within 4 days after transplantation, both for fresh and cryopreserved tissue. However, large follicular loss has been observed in the first days post-transplantation, especially in cryopreserved tissues.

Tài liệu tham khảo

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