Investigation of the mesoscale structure and volumetric features of biofilms using optical coherence tomography

Biotechnology and Bioengineering - Tập 107 Số 5 - Trang 844-853 - 2010
Michael Wagner1,2,3, Danial Taherzadeh1,2,3, Christoph Haisch4, Harald Horn1,2,3
1Institute of Water Quality Control, Technische Universität München, Am Coulombwall, D-85748 Garching, Germany
2fax: +49-89-289-13718
3telephone: +49-89-289-13700
4Institute of Hydrochemistry, Chair for Analytical Chemistry, Technische Universität München, Munich, Germany

Tóm tắt

AbstractOptical coherence tomography (OCT) was successfully applied to visualize the mesoscale structure of three different heterotrophic biofilms. For this purpose, biofilm volumes of 4 × 4 × 1.6 mm3 were scanned with spatial resolutions lower than 20 µm within an acquisition time of 2 min. A heterogeneous structure was detected for biofilms cultivated in laminar as well as transient flow conditions. The structure was found to be more homogeneous for the biofilm grown in turbulent flow. This biofilm structure was characterized by a volumetric porosity of 0.36, whereas the porosity calculated for biofilms grown in laminar and transient conditions was 0.65. These results were directly generated from the distribution of porosity calculated from the OCT images acquired and can be linked to structural properties. Up to now, the mesoscale biofilm structure was only observable with time‐consuming and expensive studies, for example, magnetic resonance microscopy. OCT will most certainly be helpful for improved understanding and prediction of biofilm physics with respect to macroscale processes, for example, mass transfer and detachment as the information about mesoscale is easily accessible using this method. In the context of this study, we show that CLSM images do not necessarily provide an accurate representation of the biofilm structure at the mesoscale. Additionally, the typical characteristic parameters obtained from CLSM image stacks differ largely from those calculated from OCT images. Nevertheless, to determine the local distribution of biofilm constituents, microscopic methods such as confocal laser scanning microscopy are required. Biotechnol. Bioeng. 2010;107: 844–853. © 2010 Wiley Periodicals, Inc.

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Biotechnology and Bioengineering

Tập 107 Số 5

844-853

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