Investigation of the mesoscale structure and volumetric features of biofilms using optical coherence tomography
Tóm tắt
Optical coherence tomography (OCT) was successfully applied to visualize the mesoscale structure of three different heterotrophic biofilms. For this purpose, biofilm volumes of 4 × 4 × 1.6 mm3 were scanned with spatial resolutions lower than 20 µm within an acquisition time of 2 min. A heterogeneous structure was detected for biofilms cultivated in laminar as well as transient flow conditions. The structure was found to be more homogeneous for the biofilm grown in turbulent flow. This biofilm structure was characterized by a volumetric porosity of 0.36, whereas the porosity calculated for biofilms grown in laminar and transient conditions was 0.65. These results were directly generated from the distribution of porosity calculated from the OCT images acquired and can be linked to structural properties. Up to now, the mesoscale biofilm structure was only observable with time‐consuming and expensive studies, for example, magnetic resonance microscopy. OCT will most certainly be helpful for improved understanding and prediction of biofilm physics with respect to macroscale processes, for example, mass transfer and detachment as the information about mesoscale is easily accessible using this method. In the context of this study, we show that CLSM images do not necessarily provide an accurate representation of the biofilm structure at the mesoscale. Additionally, the typical characteristic parameters obtained from CLSM image stacks differ largely from those calculated from OCT images. Nevertheless, to determine the local distribution of biofilm constituents, microscopic methods such as confocal laser scanning microscopy are required. Biotechnol. Bioeng. 2010;107: 844–853. © 2010 Wiley Periodicals, Inc.
Từ khóa
Tài liệu tham khảo
Bryers JD., 2000, Biofilms II—Process analysis and applications
Characklis WG., 1989, Laboratory biofilm reactors
Manz B, 2005, Investigation of biofilm structure, flow patterns and detachment with magnetic resonance imaging, Water Sci Technol, 52, 1, 10.2166/wst.2005.0173
Staudt C, 2003, Biofilms in industry, medicine & environmental biotechnology, 308
WangR LiuX XiongGL XueP ChenDY.2006.Full‐field OCT combining with fluorescent microscopy—Art. no. 60470n. In: Xu K Luo Q Xing D Priezzhev AV Tuchin VV editors. Fourth International Conference on Photonics and Imaging in Biology and Medicine Pts 1 and 2 Proceedings of the Society of Photo‐Optical Instrumentation Engineers (SPIE) Vol. 6047. SPIE‐Int Soc Optical Engineering Bellingham p. N470.
Wuertz S, 2008, Biofilms in wastewater treatment: An interdisciplinary approach