Investigation of Hanwoo-specific structural variations using whole-genome sequencing data

Springer Science and Business Media LLC - Tập 41 - Trang 233-240 - 2018
Jangho Park1, Wonseok Shin1, Seyoung Mun1, Man Hwan Oh1, Dajeong Lim2, Dong-Yep Oh3, Youngjune Bhak4, Jong Bhak4, Yong-Soo Park5, Kyudong Han1
1Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea
2Animal Genomics and Bioinformatics Division, Wanju-gun, Republic of Korea
3Livestock Research Institute, Yeongju, Republic of Korea
4Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea
5Department of Equine Industry, Korea National College of Agriculture and Fisheries, Jeonju, Republic of Korea

Tóm tắt

The total length of the cattle genome is approximately ~ 3 billion base pairs. About half of the bovine genome (46.5%) is composed of transposable elements (TEs). The TEs could be a major source of genomic structural variations (SVs) between cattle breeds. These SVs have led to genomic fluidity and rearrangements between interspecies. TE-mediated insertion and deletion events could have a strong influence on the bovine genome. This study aimed to investigate TE-mediated deletion events that are common to 12 Hanwoo genome resequencing data. We compared 12 Hanwoo genome resequencing data with the cattle reference genome (Bos taurus_UMD_3.1.1) and six other open source data (2 Jersey, 2 Holstein, 2 Angus). By using BreakDancer program, the common SVs to the 12 Hanwoo genomes were detected. A total of 299 Hanwoo-specific SV candidates were detected. Among them, 56 Hanwoo-specific TE-mediated deletion candidate loci were validated by PCR and Sanger sequencing. Finally, we identified one locus, DEL_96, which is an authentic Hanwoo-specific deletion. The DEL_96 event occurred by nonallelic homologous end-joining between LINE (BovB) and unique sequence with 1 bp microhomology. The 370 bp deletion event appeared to be only in the Hanwoo individuals after the divergence of Hanwoo and Holstein lineages. Our study showed that one of the SVs, TE-mediated deletion, could be utilized as a molecular maker to distinguish between Hanwoo and Holstein.

Tài liệu tham khảo

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