Investigation of Functional IL-10 Gene Polymorphism and IL-10 Levels in Acute Graft-Versus-Host Disease

Springer Science and Business Media LLC - Tập 30 - Trang 465-473 - 2010
Renata Gonçalves Resende1, Jeane de Fátima Correia-Silva1, Telma Cristina Arão1, Tarcília Aparecida Silva1, Mauro Henrique Nogueira Guimarães Abreu2, Henrique Bittencourt3, Ricardo Santiago Gomez1,4
1Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
2Department of Preventive Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
3Stem Cell Transplant Unit, Hospital das Clinicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
4Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

Tóm tắt

Graft-versus-host disease (GVHD) represents a major complication in allogeneic hematopoietic stem cell transplant (allo-HSCT) recipients. Although studies have been conducted concerning the investigation of cytokine polymorphisms in the development of acute GVHD (aGVHD), the contribution of recipients and donors as regards cytokine levels has not yet been thoroughly assessed. The aim of this study was to investigate the impact of IL-10 polymorphisms on cytokine levels in blood and saliva, in addition to the occurrence and severity of aGVHD. Fifty-eight consecutive allo-HSCT recipients and their donors were included in this prospective study. Saliva and/or blood samples were obtained from recipients and donors to determine IL10 polymorphisms. The IL-10 levels in the blood and saliva were also assessed. The samples were collected from seven days before transplant (day −7) to 100 days after allo-HSCT (day +100), once a week or until the death of recipient. No association was found between recipient and donor IL10 polymorphism and IL-10 levels in the saliva with aGVHD. In contrast, IL-10 levels in the blood were associated with the occurrence of aGVHD. The high producer phenotype in the recipient was also associated with high levels of IL-10 in the blood and saliva. Although IL10 polymorphisms were not associated with the occurrence and severity of aGVHD, the genetic background of the recipient did in fact influence the production of the cytokine. Furthermore, as IL-10 levels in the blood were associated with the disease development, this parameter may well be a useful predictor of aGVHD development.

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