Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression

Journal of Molecular Biology - Tập 331 Số 3 - Trang 585-592 - 2003
Yuichi Koga1, Katsuya Kato2, Hideo Nakano3, Tsuneo Yamané3
1New Energy and Industrial Technology Development Organization, Sunshine 60 Bldg, 3-1-1 Higashi Ikebukuro, Toshima-ku, Tokyo 170-6028, Japan
2Bio-functional Ceramics Group, Ceramics Research Institute, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora, Shimo-shidami, Moriyama-ku, Nagoya 463-8560, Japan
3Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

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Tài liệu tham khảo

Ottosson, 2001, Rational design of enantioselective enzymes requires considerations of entropy, Protein Sci., 10, 1769, 10.1110/ps.13501

Tumoi, 1999, Molecular basis for enantioselctivity of lipase from Pseudomonas cepacia toward primary alcohols. Modeling, kinetics, and chemical modification of Tyr29 to increase or decrease enantioselectivity, J. Org. Chem., 64, 2638, 10.1021/jo981783y

Svendsen, 2000, Lipase protein engineering, Biochim. Biophys. Acta, 1543, 223, 10.1016/S0167-4838(00)00239-9

Magnusson, 2001, Creation of an enantioselective hydrolase by engineered substrate-assisted catalysis, J. Am. Chem. Soc., 123, 4354, 10.1021/ja015604x

Rotticci, 2001, Improved enantioselectivity of a lipase by rational protein engineering, ChemBioChem, 2, 766, 10.1002/1439-7633(20011001)2:10<766::AID-CBIC766>3.0.CO;2-K

May, 2000, Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine, Nature Biotechnol., 18, 317, 10.1038/73773

Reetz, 2001, Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis, Angew. Chem. Int. Ed., 40, 3589, 10.1002/1521-3773(20011001)40:19<3589::AID-ANIE3589>3.0.CO;2-X

Zha, 2001, Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution, Chem. Commun., 2664, 10.1039/b109913m

Iizumi, 1991, Cloning, nucleotide sequencing, and expression in Escherichia coli of a lipase and its activator genes from Pseudomonas sp. KWI-56, Agric. Biol. Chem., 55, 2349, 10.1271/bbb1961.55.2349

Iizumi, 1994, Role of the gene encoding lipase activator from Pseudomonas sp. strain KWI-56 in in vitro activation of lipase, Biosci. Biotechnol. Biochem., 58, 1023, 10.1271/bbb.58.1023

Swartz, 2001, Advances in Escherichia coli production of therapeutic proteins, Curr. Opin. Biotechnol., 12, 195, 10.1016/S0958-1669(00)00199-3

Yang, 2000, In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase, J. Bacteriol., 182, 295, 10.1128/JB.182.2.295-302.2000

Yang, 2002, Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site, Protein Eng., 15, 147, 10.1093/protein/15.2.147

Nakano, 2003, Seibutsu-kougaku Kaishi, 81, 71

Rungpragayphan, 2002, High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression, J. Mol. Biol., 318, 395, 10.1016/S0022-2836(02)00094-3

Koga, 2002, In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecular PCR and cell-free protein synthesis, J. Biosci. Bioeng., 94, 84, 10.1016/S1389-1723(02)80122-0

Kim, 1997, The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor, Structure, 5, 173, 10.1016/S0969-2126(97)00177-9

Rungpragayphan, 2003, PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries, FEBS Letters, 540, 147, 10.1016/S0014-5793(03)00251-5

Lang, 1998, Structural basis of the chiral selectivity of Pseudomonas cepacia lipase, Eur. J. Biochem., 254, 333, 10.1046/j.1432-1327.1998.2540333.x

Hœffner, 1998, Molecular modeling of the enantioselectivity in lipase-catalyzed transesterification reactions, Biophys. J., 74, 1251, 10.1016/S0006-3495(98)77839-7

Imamura-Miyazaki, 2003, Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase, Protein Eng.

Maehara, 1999, Analysis of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitroficans, J. Bacteriol., 181, 2914, 10.1128/JB.181.9.2914-2921.1999

Kovach, 1994, pBBR1MCS: a broad-host range cloning vector, Bio/Techniques, 16, 800