Introduction of Pea DNA Helicase 45 into Sugarcane (Saccharum spp. Hybrid) Enhances Cell Membrane Thermostability and Upregulation of Stress-Responsive Genes Leads to Abiotic Stress Tolerance

Sruthy Maria Augustine1, Jitendra Narayan1, Divya P. Syamaladevi2, C. Appunu1, Chakravarthi Mohan1, V. Ravichandran3, Narendra Tuteja4, N. Subramonian1
1Sugarcane Breeding Institute (ICAR), Tamil Nadu, India
2Indian Grass and Fodder Research Institute Regional Station, Avikanagar, India
3Department of Rice, Tamil Nadu Agricultural University, Coimbatore, India
4International Centre for Genetic Engineering and Biotechnology, New Delhi, India

Tóm tắt

Từ khóa


Tài liệu tham khảo

Amin, M., Elias, S. M., Hossain, A., Ferdousi, A., Rahman, S., Tuteja, N., & Seraj, Z. I. (2011). Over-expression of a DEAD-box helicase, PDH45, confers both seedling and reproductive stage salinity tolerance to rice (Oryza sativa L.). Molecular Breeding. doi: 10.1007/s11032-011-9625-3 .

Araus, J. L., Slafer, G. A., Reynolds, M. P., & Royo, C. (2002). Plant breeding and water relations in C3 cereals: What should we breed for? Annals of Botany (London), 89, 925–940.

Arvinth, S., Arun, S., Selvakesavan, R. K., Srikanth, J., Mukunthan, N., Ananda Kumar, P., et al. (2010). Genetic transformation and pyramiding of aprotinin expressing sugarcane with cry1Ab for shoot borer (Chilo infuscatellus) resistance. Plant Cell Reports, 29, 383–395.

Barrs, H. D., & Weatherley, P. E. (1962). A reexamination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15, 413–428.

Blum, A. (1998). Improving wheat grain filling under stress by stem reserve mobilization. Euphytica, 100, 77–83.

Borrell, A. K., Hammer, G. L., & Douglas, A. C. L. (2000). Does maintaining green leaf area in sorghum improve yield under drought? Leaf growth and senescence. Crop Science, 40, 1026–1037.

Boyer, J. S. (1982). Plant productivity and environmental. Science, 218, 443–448.

Bray, E. A. (1997). Plant responses to water deficit. Trends in Plant Science, 2, 48–54.

Chandra Babu, R., Zhang, J. X., Blum, A., Ho, D. T. H., Wu, R., & Nguyen, H. T. (2004). HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Science, 166, 855–862. doi: 10.1016/j.plantsci.2003.11.023 .

Chomczynski, P., & Mackey, K. (1995). Modification of the TRI reagent DNA/protein isolation procedure for isolation of RNA from polysaccharide and proteoglycan rich sources. BioTechniques, 19, 942–945.

Crafts-Brandner, S. J., Below, F. E., Harper, J. E., & Hageman, R. H. (1984). Effects of pod removal on metabolism and senescence of nodulating and nonnodulating soybean isolines: II. Enzymes and Chlorophyll. Plant Physiology, 75(2), 318–322.

Dang, H. Q., Tran, N. Q., Gill, S. S., Tuteja, R., & Tuteja, N. (2011). A single subunit MCM6 from pea promotes salinity stress tolerance without affecting yield. Plant Molecular Biology, 76, 19–34.

Engelbrecht, B. M. J., Melvin, T. T., & Thomas, A. K. (2007). Visual assessment of wilting as a measure of leaf water potential and seedling drought survival. Journal of Tropical Ecology, 23, 497–500.

Fan, L., Zheng, S., & Wang, X. (1997). Antisense suppression of phospholipase Da retards abscisic acid and ethylene-promoted senescence of postharvest Arabidopsis leaves. The Plant Cell, 9, 2183–2196.

Haussmann, B., Mahalakshmi, V., Reddy, B., Seetharama, N., Hash, C., & Geiger, H. (2002). QTL mapping of stay-green in two sorghum recombinant inbred populations. Theoretical and Applied Genetics, 106, 133–142.

Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., & Shinozaki, K. (1999). Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress inducible transcription factor. Nature Biotechnology, 17, 287–291.

Kovtun, Y., Chiu, W. L., Tena, G., & Sheen, J. (2000). Functional analysis of oxidative stress-activated MAPK cascade in plants. Proceedings of the National Academy of Sciences, 97, 2940–2945.

Li, R. H., Guo, P. G., Michael, B., Stefania, G., & Salvatore, C. (2006). Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agricultural Sciences in China, 5(10), 751–757.

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402–408.

Manjulatha, M., Sreevathsa, R., Kumar, A. M., Sudhakar, C., Prasad, T. G., Tuteja, N., & Udayakumar, M. (2014). Overexpression of a pea DNA helicase (PDH45) in peanut (Arachis hypogaea L.) confers improvement of cellular level tolerance and productivity under drought stress. Molecular Biotechnology, 56(2), 111–125.

Martineau, J. R., Specht, J. E., Williams, J. H., & Sullivan, C. Y. (1979). Temperature tolerance in soybeans. I. Evaluation of a technique for assessing cellular membrane thermostability. Crop Science, 19, 75–78.

Moradi, F., & Ismail, A. M. (2007). Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Annals of Botany, 99, 1161–1173.

Owttrim, G. W. (2013). RNA helicases: Diverse roles in prokaryotic response to abiotic stress. Plant Signaling & Behavior, 10(1), 95–109.

Pham, X. H., Reddy, M. K., Ehtesham, N. Z., Matta, B., & Tuteja, N. (2000). A DNA helicase from Pisum sativum is homologous to translation initiation factor and stimulates topoisomerase I activity. The Plant Journal, 24, 219–229.

Phillip, A., Syamaladevi, D. P., Chakravarthy, M., Gopinath, K., & Subramonian, N. (2013). 5′ Regulatory region of ubiquitin 2 gene from Porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots. Plant Cell Reports, 32, 1199–1210.

Sahoo, R. K., Gill, S. S., & Tuteja, N. (2012). Pea DNA helicase 45 promotes salinity stress tolerance in IR64 rice with improved yield. Plant Signaling & Behavior, 7, 1037–1041.

Sahoo, R. K., Gill, S. S., & Tuteja, N. (2012). Pea DNA helicase 45 promotes salinity stress tolerance in IR64 rice with improved yield. Plant Signaling & Behavior, 7(8), 1042–1046.

Saijo, Y., Hata, S., Kyozuka, J., Shimamoto, K., & Izui, K. (2000). Over expression of a single Ca2+ dependent protein kinase confers both cold and salt/drought tolerance on rice plants. The Plant Journal, 23, 319–327.

Sambrook, J., & Russel, D. W. (1989). Molecular cloning: A laboratory manual (III ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Sanan Mishra, N., Pham, X. H., Sopory, S. K., & Tuteja, N. (2005). Pea DNA helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield. Proceedings of the National Academy of Sciences of the United States of America, 102, 509–514.

Singh, S., Modi, M. K., Gill, S. S., & Tuteja, N. (2012). Rice: Genetic engineering approaches for abiotic stress tolerance retrospects and prospects. In N. Tuteja, S. S. Gill, & R. Tuteja (Eds.), Improving crop productivity in sustainable agriculture (pp. 203–225). Germany: Wiley-VCH Verlag GmbH and Co, KGaA.

Tuteja, N., & Tuteja, R. (2004). Prokaryotic and eukaryotic DNA helicases. Essential molecular motor proteins for cellular machinery. European Journal of Biochemistry, 271, 1835–1848.

Tuteja, N., Gill, S. S., & Tuteja, R. (2012). Helicases in improving abiotic stress tolerance in crop plants. In N. Tuteja, S. S. Gill, A. F. Tiburcio, & R. Tuteja (Eds.), Improving crop resistance to abiotic stress (pp. 433–445). Germany: Wiley-VCH Verlag GmbH and Co, KGaA.

Tuteja, N., Singh, L. P., Gill, S. S., & Tuteja, R. (2012). Salinity stress: A major constraint in crop production. In N. Tuteja, S. S. Gill, A. F. Tiburcio, & R. Tuteja (Eds.), Improving crop resistance to abiotic stress (pp. 71–96). Germany: Wiley-VCH Verlag GmbH and Co, KGaA.

Umate, P., Tuteja, R., & Tuteja, N. (2010). Genome-wide analysis of helicase gene family from rice and Arabidopsis: A comparison with yeast and human. Plant Molecular Biology, 73, 449–465.

Vashisht, A. A., & Tuteja, N. (2006). Stress responsive DEAD-box helicases: A new pathway to engineer plant stress tolerance. Journal of Photochemistry and Photobiology B: Biology, 84, 150–160.

Veena Reddy, V. S., & Sopory, S. K. (1999). Glyoxalase I from (Brassica juncea) molecular cloning, regulation, and its over expression confer tolerance in transgenic tobacco under stress. The Plant Journal, 17, 385–395.

Verma, V., Foullces, M. J., Worland, A. J., Sylvester-Bradley, R., Caligari, P. D. S., & Snape, J. W. (2004). Mapping quantitative trait loci for flag leaf senescence as a yield determinant in winter wheat under optimal and drought-stressed environments. Euphytica, 135, 255–263.

Winicov, I. (2000). Alfin1 transcription factor over expression enhances plant root growth under normal and saline conditions and improves salt tolerance in alfalfa. Planta, 210, 416–422.

Wu, Y. (2012). Unwinding and rewinding double faces of helicase? Journal of Nucleic Acids, 2012, 140601. doi: 10.1155/2012/140601 .

Xu, D., Duan, X., Wang, B., Hong, B., Ho, T., & Wu, R. (1996). Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology, 110, 249–257.

Zhang, H. K., Hodson, J. N., Williams, J. P., & Blumwald, E. (2001). Engineering salt tolerant (Brassica) plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proceedings of the National Academy of Sciences, 98, 12832–12836.

Zhang, Z. H., Liu, Q., Song, H. X., Rong, X. M., & Ismail, A. M. (2012). Responses of different rice (Oryza sativa L.) genotypes to salt stress and relation to carbohydrate metabolism and chlorophyll content. African Journal of Agricultural Research, 7, 19–27.