Intravascular staining for discrimination of vascular and tissue leukocytes

Nature Protocols - Tập 9 Số 1 - Trang 209-222 - 2014
Kristin G. Anderson1, Katrin D. Mayer-Barber2, Heungsup Sung3, Lalit K. Beura1, Britnie R. James4, Justin J. Taylor1, Lindor Qunaj1, Thomas S. Griffith4, Vaiva Vezys1, Daniel L. Barber5, David Masopust1
1Department of Microbiology, Center for Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
2Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, US National Institutes of Health (NIH), Bethesda, Maryland, USA.
31] Department of Microbiology, Center for Immunology, University of Minnesota, Minneapolis, Minnesota, USA. [2].
4Department of Urology, Center for Immunology, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA.
5T lymphocyte Biology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.

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Andrian von, U.H. & Mackay, C.R. T-cell function and migration. Two sides of the same coin. N. Engl. J. Med. 343, 1020–1034 (2000).

Islam, S.A. & Luster, A.D. T cell homing to epithelial barriers in allergic disease. Nat. Med. 18, 705–715 (2012).

Sigmundsdottir, H. & Butcher, E.C. Environmental cues, dendritic cells and the programming of tissue-selective lymphocyte trafficking. Nat. Immunol. 9, 981–987 (2008).

Manicassamy, S. & Pulendran, B. Dendritic cell control of tolerogenic responses. Immunol. Rev. 241, 206–227 (2011).

Wynn, T.A., Chawla, A. & Pollard, J.W. Macrophage biology in development, homeostasis and disease. Nature 496, 445–455 (2013).

Lefrançois, L. & Puddington, L. Intestinal and pulmonary mucosal T cells: local heroes fight to maintain the status quo. Annu. Rev. Immunol. 24, 681–704 (2006).

Zhang, N. & Bevan, M.J. CD8+ T cells: foot soldiers of the immune system. Immunity 35, 161–168 (2011).

Sheridan, B.S. & Lefrançois, L. Regional and mucosal memory T cells. Nat. Immunol. 12, 485–491 (2011).

Mueller, S.N., Gebhardt, T., Carbone, F.R. & Heath, W.R. Memory T cell subsets, migration patterns, and tissue residence. Annu. Rev. Immunol. 31, 137–161 (2013).

Gebhardt, T., Mueller, S.N., Heath, W.R. & Carbone, F.R. Peripheral tissue surveillance and residency by memory T cells. Trends Immunol. 34, 27–32 (2013).

Guilliams, M., Lambrecht, B.N. & Hammad, H. Division of labor between lung dendritic cells and macrophages in the defense against pulmonary infections. Mucosal Immunol. 6, 464–473 (2013).

Galkina, E. et al. Preferential migration of effector CD8+ T cells into the interstitium of the normal lung. J. Clin. Invest. 115, 3473–3483 (2005).

Anderson, K.G. et al. Cutting edge: intravascular staining redefines lung CD8 T cell responses. J. Immunol. 189, 2702–2706 (2012).

Geissmann, F. et al. Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids. PLoS Biol. 3, e113 (2005).

Lee, W.-Y. et al. An intravascular immune response to Borrelia burgdorferi involves Kupffer cells and iNKT cells. Nat. Immunol. 11, 295–302 (2010).

Thomas, S.Y. et al. PLZF induces an intravascular surveillance program mediated by long-lived LFA-1-ICAM-1 interactions. J. Exp. Med. 208, 1179–1188 (2011).

Scanlon, S.T. et al. Airborne lipid antigens mobilize resident intravascular NKT cells to induce allergic airway inflammation. J. Exp. Med. 208, 2113–2124 (2011).

Carlin, L.M. et al. Nr4a1-dependent Ly6Clow monocytes monitor endothelial cells and orchestrate their disposal. Cell 153, 362–375 (2013).

Hogg, J.C. et al. Erythrocyte and polymorphonuclear cell transit time and concentration in human pulmonary capillaries. J. Appl. Physiol. 77, 1795–1800 (1994).

Segel, G.B., Cokelet, G.R. & Lichtman, M.A. The measurement of lymphocyte volume: importance of reference particle deformability and counting solution tonicity. Blood 57, 894–899 (1981).

Berlin-Rufenach, C. et al. Lymphocyte migration in lymphocyte function-associated antigen (LFA)-1-deficient mice. J. Exp. Med. 189, 1467–1478 (1999).

Cose, S., Brammer, C., Khanna, K.M., Masopust, D. & Lefrançois, L. Evidence that a significant number of naive T cells enter non-lymphoid organs as part of a normal migratory pathway. Eur. J. Immunol. 36, 1423–1433 (2006).

Harp, J.R. & Onami, T.M. NaÏve T cells re-distribute to the lungs of selectin ligand deficient mice. PLoS ONE 5, e10973 (2010).

Inman, C.F., Murray, T.Z., Bailey, M. & Cose, S. Most B cells in non-lymphoid tissues are naive. Immunol. Cell Biol. 90, 235–242 (2012).

Caucheteux, S.M., Torabi-Parizi, P. & Paul, W.E. Analysis of naive lung CD4 T cells provides evidence of functional lung to lymph node migration. Proc. Natl. Acad. Sci. USA 110, 1821–1826 (2013).

Neyt, K., Perros, F., GeurtsvanKessel, C.H., Hammad, H. & Lambrecht, B.N. Structure, development and function of tertiary lymphoid organs in infection and autoimmunity. Trends Immunol. 33, 297–305 (2012).

Hart, B.A., Harmsen, A.G., Low, R.B. & Emerson, R. Biochemical, cytological, and histological alterations in rat lung following acute beryllium aerosol exposure. Toxicol. Appl. Pharmacol. 75, 454–465 (1984).

Woodland, D.L. & Randall, T.D. Anatomical features of anti-viral immunity in the respiratory tract. Semin. Immunol. 16, 163–170 (2004).

Moyron-Quiroz, J., Rangel-Moreno, J., Carragher, D.M. & Randall, T.D. The function of local lymphoid tissues in pulmonary immune responses. Adv. Exp. Med. Biol. 590, 55–68 (2007).

Carragher, D.M., Rangel-Moreno, J. & Randall, T.D. Ectopic lymphoid tissues and local immunity. Semin. Immunol. 20, 26–42 (2008).

Randall, T.D. Chapter 7—bronchus-associated lymphoid tissue (BALT): structure and function. synthetic vaccines. (eds. Fagarasan, S. & Cerutti, A.) in Advances in Immunology Vol. 107, 187–241 (Elsevier, 2010).

Barletta, K.E. et al. Leukocyte compartments in the mouse lung: distinguishing between marginated, interstitial, and alveolar cells in response to injury. J. Immunol. Methods 375, 100–110 (2012).

Radu, M. & Chernoff, J. An in vivo assay to test blood vessel permeability. J. Vis. Exp. 16, e50062 (2013).

Hale, J.S. et al. Distinct memory CD4+ T cells with commitment to T follicular helper- and T helper 1-cell lineages are generated after acute viral infection. Immunity 38, 805–817 (2013).

Mayer-Barber, K.D. et al. Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection. Immunity 35, 1023–1034 (2011).

Norian, L.A. et al. Eradication of metastatic renal cell carcinoma after adenovirus-encoded TNF-related apoptosis-inducing ligand (TRAIL)/CpG immunotherapy. PLoS ONE 7, e31085 (2012).

James, B.R. et al. Diet-induced obesity alters dendritic cell function in the presence and absence of tumor growth. J. Immunol. 189, 1311–1321 (2012).

Casey, K.A. et al. Antigen-independent differentiation and maintenance of effector-like resident memory T cells in tissues. J. Immunol. 188, 4866–4875 (2012).

Masopust, D., Vezys, V., Marzo, A.L. & Lefrançois, L. Preferential localization of effector memory cells in nonlymphoid tissue. Science 291, 2413–2417 (2001).

Sharrow, S.O. Overview of flow cytometry. Curr. Protoc. Immunol. 5, 5.1 (2002).

Roederer, M. Multiparameter FACS analysis. Curr. Protoc. Immunol. 5, 5.8 (2002).

Daniels, M.A. & Jameson, S.C. Critical role for CD8 in T cell receptor binding and activation by peptide/major histocompatibility complex multimers. J. Exp. Med. 191, 335–346 (2000).

Arnon, T.I., Horton, R.M., Grigorova, I.L. & Cyster, J.G. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. Nature 493, 684–688 (2013).

Allman, D. & Pillai, S. Peripheral B cell subsets. Curr. Opin. Immunol. 20, 149–157 (2008).

Pereira, J.P., Kelly, L.M. & Cyster, J.G. Finding the right niche: B-cell migration in the early phases of T-dependent antibody responses. Int. Immunol. 22, 413–419 (2010).

Batista, F.D. & Harwood, N.E. The who, how and where of antigen presentation to B cells. Nat. Rev. Immunol. 9, 15–27 (2009).

Gebhardt, T. et al. Memory T cells in nonlymphoid tissue that provide enhanced local immunity during infection with herpes simplex virus. Nat. Immunol. 10, 524–530 (2009).

Teijaro, J.R. et al. Cutting edge: tissue-retentive lung memory CD4 T cells mediate optimal protection to respiratory virus infection. J. Immunol. 187, 5510–5514 (2011).

Wissinger, E., Goulding, J. & Hussell, T. Immune homeostasis in the respiratory tract and its impact on heterologous infection. Semin. Immunol. 21, 147–155 (2009).

Wolf, A.J. et al. Mycobacterium tuberculosis infects dendritic cells with high frequency and impairs their function in vivo. J. Immunol. 179, 2509–2519 (2007).

Geissmann, F. et al. Development of monocytes, macrophages, and dendritic cells. Science 327, 656–661 (2010).

Gabrilovich, D.I. & Nagaraj, S. Myeloid-derived suppressor cells as regulators of the immune system. Nat. Rev. Immunol. 9, 162–174 (2009).

Ko, J.S. et al. Direct and differential suppression of myeloid-derived suppressor cell subsets by sunitinib is compartmentally constrained. Cancer Res. 70, 3526–3536 (2010).

Finke, J. et al. MDSC as a mechanism of tumor escape from sunitinib mediated anti-angiogenic therapy. Int. Immunopharmacol. 11, 856–861 (2011).

Dietlin, T.A. et al. Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion. J. Leukoc. Biol. 81, 1205–1212 (2007).

Movahedi, K. et al. Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cell-suppressive activity. Blood 111, 4233–4244 (2008).

Reutershan, J. Sequential recruitment of neutrophils into lung and bronchoalveolar lavage fluid in LPS-induced acute lung injury. Am. J. Physiol. Lung Cell. Mol. Physiol. 289, L807–L815 (2005).

Kang, S.S. et al. Migration of cytotoxic lymphocytes in cell cycle permits local MHC I-dependent control of division at sites of viral infection. J. Exp. Med. 208, 747–759 (2011).

Donaldson, J.G. Immunofluorescence staining. Curr. Protoc. Cell Biol. 4.3.1–4.3.6 (2001).

Combs, C.A. Fluorescence Microscopy: A Concise Guide to Current Imaging Methods (John Wiley & Sons, 2001).

Herman, B. Fluorescence microscopy. Curr. Protoc. Immunol. 48, 21.2.1–21.2.10 (2002).