Intranuclear fibrillar material in cereal pollen mother cells
Tóm tắt
Ultrastructural studies of cereal anthers found intranuclear bundles of microfilaments in pollen mother cells (PMCs) but not elsewhere. The ultrastructure, distribution, and behaviour of this fibrillar material (FM) are described. FM was seen in all 19 genotypes studied comprising Aegilops, Triticum, Secale, Hordeum and Avena species, which together included haploid, diploid and allo-and autopolyploid, and natural and synthetic polyploid examples. Detailed studies in diploid S. cereale, and hexaploid T. aestivum and Triticale showed that FM was present in PMC nuclei during premeiotic interphase, leptotene and zygotene but not at pachytene and later meiotic stages. Moreover, it was most abundant at late premeiotic interphase in T. aestivum, and at leptotene in S. cereale and Triticale, when it occurred in up to 100% of sampled PMC nuclei in an anther. Although FM and synaptonemal complex (SC) occurred together in some PMC nuclei at later stages, FM was present long before SC, and reached its peak of abundance before SC did. Bundles of FM often formed links at their ends between either two masses of chromatin, or more rarely, between chromatin and the nuclear membrane. Individual bundles of FM varied in length but showed roughly similar ranges of lengths and widths in these three species. They were up to about 0.2 μm in diameter and about 3 μm in length, equivalent to about 20% of the maximum diameter of the nuclei containing them. Reconstructions of PMC nuclei indicated that FM was never associated with centromeres but was sometimes, and perhaps usually, associated with telomeric or sub-telomeric chromosome segments. The function of FM is unknown but its possible role is discussed in relation to (1) previously described intranuclear inclusions in meiocytes and (2) the cytogenetics and developmental behaviour of meiotic nuclei in the wheat comparium. As FM was a constant and characteristic structural component of PMC nuclei, its presence is probably of functional significance to the meiotic process. If so, it may function before, and over greater distances, than SC in establishing or maintaining the coorientation of chromosomes prerequisite for normal chromosome pairing. As FM was most abundant at stages when major chromosome movements occur, yet its distribution was non-centromeric, it is suggested that it may function in the attachment and movement of telomeres at the nuclear membrane formed after premeiotic mitosis. The possibility that a bundle of FM normally links corresponding sites on two homologues is considered.
Tài liệu tham khảo
Bayliss, M.W., Riley, R.: An analysis of temperature-dependent asynapsis in Triticum aestivum. Genet. Res. Camb. 20, 193–200 (1972a)
Bayliss, M.W., Riley, R.: Evidence of premeiotic control of chromosome pairing in Triticum aestivum. Genet. Res. Camb. 20, 201–212 (1972b)
Bennett, M.D., Smith, J.B.: The effects of polyploidy on meiotic duration and pollen development in cereal anthers. Proc. roy. Soc. Lond. B, 181, 81–107 (1972)
Bennett, M.D., Chapman, V., Riley, R.: The duration of meiosis in pollen mother cells of wheat, rye and Triticale. Proc. roy. Soc. Lond. B, 178, 259–175 (1971)
Bennett, M.D., Rao, M.K., Smith, J.B., Bayliss, M.W.: Cell development in the anther, the ovule, and the young seed of Triticum aestivum L. var. Chinese Spring. Philos. Trans. R. Soc. Lond. B, 266, 39–81 (1973)
Bennett, M.D., Stern, H., Woodward, M.: Chromatin attachment to nuclear membrane of wheat pollen mother cells. Nature (Lond.) 252, 395–396 (1974)
Bogdanov, Y.F.: Formation of cytoplasmic synaptonemal-like polycomplexes at leptotene and normal synaptonemal complexes at zygotene in Ascaris suum male meiosis. Chromosoma (Berl.) 61, 1–21 (1977)
Bottke, W.: Chromosome-associated paracrystalline nuclear inclusions in the spermatocytes of a pulmonate snail, Planorbarius corneus L. Chromosoma (Berl.) 55, 273–287 (1976)
Bowman, J.G., Rajhathy, T.: Fusion of chromocentres in premeiotic interphase of Secale cereale and its possible relationship to chromosome pairing. Can. J. Genet. Cytol. 19, 313–321 (1977)
Church, K., Moens, P.B.: Centromere behaviour during iuterphase and meiotic prophase in Allium fistulosum from 3-D, E.M. reconstruction. Chromosoma (Berl.) 56, 249–263 (1976)
Comings, D.E.: The rationale for an ordered arrangement of chromatin in the interphase nucleus. Amer. J. hum. Genet. 20, 440–459 (1968)
Dover, G.A.: The organisation and polarity of pollen mother cells of Triticum aestivum. J. Cell Sci. 11, 699–711 (1972)
Dover, G.A., Riley, R.: The effect of spindle inhibitors applied before meiosis on meiotic chromosome pairing. J. Cell Sci. 12, 143–161 (1973)
Dover, G.A., Riley, R.: Inferences from genetical evidence on the course of meiotic chromosome pairing in plants. Philos. Trans. R. Soc. Lond. B 277, 313–326 (1977)
Driscoll, C.J., Darvey, N.L., Barber, H.N.: Effect of colchicine on meiosis in hexaploid wheat. Nature (Lond.) 216, 687–688 (1967)
Fiil, A.: Oogenesis in the malaria mosquito Anopheles gambiae. Cell Tissue Res. 167, 23–35 (1976)
Fiil, A., Moens, P.B.: The development, structure and function of modified synaptonemal complexes in mosquito oocytes. Chromosoma (Berl.) 41, 37–62 (1973)
Feldman, M.: Regulation of somatic association and meiotic pairing in common wheat, pp. 169–178. In: Third Int. Wheat Genet. Symp. 1968
Feldman, M., Mello-Sampayo, T., Sears, E.R.: Somatic association in Triticum aestivum. Proc. Natl. Acad. Sci. USA 56, 1192–1199 (1966)
Forer, A., Jackson, W.T.: Actin filaments in the endosperm mitotic spindles in a higher plant, Haemanthus katherinae Baker. Cytobiologie 12, 199–214 (1976)
Franke, W.W., Heath, W., Van Der Woude, W.J., Morré, D.: Tubular and filamentous structures in pollen tubes: Possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles. Planta (Berl.) 105, 317–341 (1972)
Fuge, H.: A crystalloid component in the meiotic spindle. Exp. Cell Res. 60, 309–313 (1970)
Gillies, C.B.: The nature and extent of synaptonemal complex formation in haploid barley. Chromosoma (Berl.) 48, 441–453 (1974)
Gillies, C.B.: An ultrastructural analysis of chromosomal pairing in maize. C. R. Trav. Lab. Carlsberg 40, 135–161 (1975)
Gillies, C.B., Hyde, B.B.: Intranucleolar bodies in maize microsporocytes. Hereditas 74, 137–140 (1973)
Gustafson, J.P., Bennett, M.D.: Preferential selection for wheat-rye substitutions in 42-chromosome triticale. Crop Sci. 16, 688–693 (1976)
Hauser, M.: The intranuclear mitosis of the ciliates Paracineta limbata and Ichthyophthirius miltifilius. Chromosoma (Berl.) 36, 157–158 (1972)
Ishikawa, H., Bischoff, R., Holtzer, H.: Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43, 312–328 (1969)
Jaworska, H., Lima-de-Faria, A.: Multiple synaptonemal complexes at the region of gene amplification in Acheta. Chromosoma (Berl.) 28, 309–327 (1969)
Kamiya, N.: Introductory remarks. In: Intern. cell biol. B.R. Brinkley, and K.R. Porter (eds.), pp. 361–366. New York: The Rockefeller University Press (1977)
Karasaki, S.: Intranuclear crystal within the phagocytes. J. Cell Biol. 25, 654–660 (1965)
Kasha, K.J., Burnham, C.R.: The location of interchange breakpoints in barley. II. Chromosome pairing and the intercross method. Can. J. Genet. Cytol. 7, 620–632 (1965)
La Cour, L.F., Wells, B.: Some morphological aspects of the synaptonemal complex in higher plants. Philos. Trans. R. Soc. Lond. B 277, 259–266 (1977)
Larter, E.N., Shebeski, L.H., McGinnis, R.C., Evans, L.E., Kaltsikes, P.J.: Rosner: A hexaploid triticale cultivar. Canad. J. Plant Sci. 50, 122–124 (1970)
McFadden, E.S., Sears, E.R.: The origin of Triticum spelta and its free-threshing hexaploid relatives. J. Hered. 37, 81–89 (1946)
McQuade, H.A., Bassett, B.: Synaptonemal complexes in premeiotic interphase of pollen mother cells of Triticum aestivum. Chromosoma (Berl.) 63, 153–159 (1977)
O'Brien, T.P., Thimann, K.V.: Intracellular fibres in oat coleoptile cells and their possible significance in cytoplasmic streaming. Proc. Natl. Acad. Sci. USA 56, 888–894 (1966)
Oliveira, L.: Ultrastructural, cytochemical and experimental studies on the cytoplasmic and nucleoplasmic inclusions of root meristematic cells of a Triticale. J. submicr. Cytol. 8, 29–39 (1976)
Parthasarathy, M.V., Muhlethaler, K.: Ultrastructure of protein tubules in differentiating sieve elements. Cytobiologie 1, 17–36 (1969)
Poux, N.: Sur l'existance de structures à arrangement périodique dans les cellules embryonnaires de Triticum vulgare Vill. (Graminées). J. Microsc. 2, 643–648 (1963)
Rasmussen, S.W.: Ultrastructural studies of spermatogenesis in Drosophila melanogaster Meigen. Z. Zellforsch. 140, 125–144 (1973)
Rasmussen, S.W.: Synaptonemal polycomplexes in Drosophila melanogaster. Chromosoma (Berl.) 49, 321–331 (1975)
Reimer, L., Roessner, A., Themann, H., Bassewitz, D.B. v.: Optical diffraction on tilting series of paracrystalline intranuclear inclusions of dog liver parenchymal cells. J. Ultrastruct. Res. 45, 356–365 (1973)
Reynold, E.S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Riley, R.: Cytogenetics of chromosome pairing. Genetics 78, 193–203 (1974)
Riley, R., Kempana, C.: The homeologous nature of the non-homologous meiotic pairing in Triticum aestivum deficient for chromosome V (5B). Heredity 18, 287–306 (1963)
Riley, R., Law, C.N.: Genetic variation in chromosome pairing. In Advanc. Genet. 13, 57–114 (1965)
Riley, R., Unrau, J., Chapman, V.: Evidence on the origin of the B genome of wheat. J. Hered. 49, 91–98 (1958)
Seïte, R.: Etude ultrastructurale de divers types d'inclusions nucléaires dans les neurones sympathiques du chat. J. Ultrastruct. Res. 30, 152–165 (1970)
Smith, U., Smith, D.S.: A microtubular complex on the epidermal nucleus of an insect, Carausius morosus. J. Cell Biol. 26, 961–967 (1965)
Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)
Srivastava, L.M., O'Brien, T.P.: On the ultrastructure of cambium and its vascular derivatives. Protoplasma 61, 257–276 (1966)
Taylor, D.L.: Dynamics of cytoplasmic structure and contractility. In: Int. cell biology (B.R. Brinkley and K.R. Porter, eds.), pp. 367–377. New York: The Rockefeller University Press 1977
Thomas, J.B., Kaltsikes, P.J.: A bouquet-like attachment plate for telomeres in leptotene of rye revealed by heterochromatin staining. Heredity 36, 155–162 (1976)
Vivier, E., André, J.: Existance d'inclusions d'ultrastructure fibrillaire dans le macronucleus de certaines souches de Paramecium caudatum Ehr. C.R. Acad. Sci. (Paris) 252, 1848–1853 (1961)
Weber, K.: Specific visualization of tubulin containing structures by immunofluorescence microscopy: cytoplasmic microtubules, vinblastine induced paracrystals and mitotic figures. In: Microtubules and microtubule inhibitors (M, Borgers, and M. de Brabander, eds.), pp. 313–325. Amsterdam: Publication Comp. North Holland. 1975
Wells, B.: A convenient technique for the collection of ultra-thin sections. Micron 5, 79–81 (1974)
Welsch, B.: Synaptonemal complex und Chromosomenstruktur in der achiasmatischen Spermatogenese von Panorpa communis. Chromosoma (Berl.) 43, 19–74 (1974)
Wenzel, G., Hoffmann, F., Thomas, E.: Increased induction and chromosome doubling of androgenetic haploid rye. Theor. Appl. Genet. 51, 81–86 (1977)
Wergin, W.P., Gruber, P.J., Newcomb, E.H.: Fine structural investigation of nuclear inclusions in plants. J. Ultrastr. Res. 30, 533–557 (1970)
Westergaard, M., Wettstein, D. von: Studies on the mechanism of crossing over. IV. The molecular organisation of the synaptinemal complex in Neottialla (Cooke) Saccardo (Ascomycetes). C.R. Trav. Lab. Carlsberg 37, 239–268 (1970)
Westergaard, M., Wettstein, D. von: The synaptinemal complex. Annu. Rev. Genet. 6, 71–110 (1972)
Wettstein, D. von: The assembly of the synaptinemal complex. Phil. Trans. roy. Soc. Lond. B 277, 235–243 (1977)
Zee, S.Y., Chambers, T.C.: Fine structure of the primary root phloem of Pisum. Aust. J. Both. 16, 37–47 (1968)