Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses

BMC Immunology - Tập 11 - Trang 1-9 - 2010
Byoung-Shik Shim1,2, Sung-Moo Park3,2, Ji-Shan Quan1,4, Dhananjay Jere1, Hyuk Chu5, Man Ki Song2, Dong Wook Kim2, Yong-Suk Jang6, Moon-Sik Yang6, Seung Hyun Han2,7, Yong-Ho Park3, Chong-Su Cho1, Cheol-Heui Yun1,8
1Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea
2Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea
3Department of Microbiology, College of Veterinary medicine, Seoul National University, Seoul, Republic of Korea
4College of Pharmacy, Yanbian University, China
5Division of Zoonoses, Center for Immunology & Pathology, National Institute of Health, Korea Center for Disease Control & Prevention, Seoul, Republic of Korea
6Division of Biological Sciences and The Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Republic of Korea
7Department of Oral Microbiology & Immunology, Dental Research Institute and BK21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea
8Center for Agricultural Biomaterials, Seoul National University, Seoul, Republic of Korea

Tóm tắt

Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 ± 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220+ cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-Ad) were increased on CD11c+ dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-γ-, TNF-α- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.

Tài liệu tham khảo

Zhong N, Zheng B, Li Y, Poon L, Xie Z, Chan K, Li P, Tan S, Chang Q, Xie J: Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China. February Lancet. 2003, 362: 1353-1358. 10.1016/S0140-6736(03)14630-2. Rota P, Oberste M, Monroe S, Nix W, Campagnoli R, Icenogle J, Penaranda S, Bankamp B, Maher K, Chen M: Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 2003, 300: 1394-1399. 10.1126/science.1085952. Li W, Moore M, Vasilieva N, Sui J, Wong S, Berne M, Somasundaran M, Sullivan J, Luzuriaga K, Greenough T: Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003, 426: 450-454. 10.1038/nature02145. Rice J, Ottensmeier C, Stevenson F: DNA vaccines: precision tools for activating effective immunity against cancer. Nature Reviews Cancer. 2008, 8: 108-120. 10.1038/nrc2326. Wang B, Ugen K, Srikantan V, Agadjanyan M, Dang K, Refaeli Y, Sato A, Boyer J, Williams W, Weiner D: Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences. 1993, 90: 4156-4160. 10.1073/pnas.90.9.4156. Yang Z, Kong W, Huang Y, Roberts A, Murphy B, Subbarao K, Nabel G: A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice. Nature. 2004, 428: 561-564. 10.1038/nature02463. Wang D, Christopher M, Nagata L, Zabielski M, Li H, Wong J, Samuel J: Intranasal immunization with liposome-encapsulated plasmid DNA encoding influenza virus hemagglutinin elicits mucosal, cellular and humoral immune responses. Journal of Clinical Virology. 2004, 31: 99-106. 10.1016/j.jcv.2004.09.013. Fagarasan S, Honjo T: Intestinal IgA synthesis: regulation of front-line body defences. Nature Reviews Immunology. 2003, 3: 63-72. 10.1038/nri982. Tumpey T, Renshaw M, Clements J, Katz J: Mucosal delivery of inactivated influenza vaccine induces B-cell-dependent heterosubtypic cross-protection against lethal influenza A H5N1 virus infection. Journal of virology. 2001, 75: 5141-5150. 10.1128/JVI.75.11.5141-5150.2001. Boussif O, Lezoualc'h F, Zanta M, Mergny M, Scherman D, Demeneix B, Behr J: A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proceedings of the National Academy of Sciences. 1995, 92: 7297-7301. 10.1073/pnas.92.16.7297. Regnstrm K, Ragnarsson E, ping-H gg rd MK, Torstensson E, Nyblom H, Artursson P: PEI a potent, but not harmless, mucosal immuno-stimulator of mixed T-helper cell response and FasL-mediated cell death in mice. Gene Therapy. 2003, 10: 1575-1583. 10.1038/sj.gt.3302054. Densmore C, Orson F, Xu B, Kinsey B, Waldrep J, Hua P, Bhogal B, Knight V: Aerosol delivery of robust polyethyleneimine-DNA complexes for gene therapy and genetic immunization. Molecular Therapy. 2000, 1: 180-188. 10.1006/mthe.1999.0021. Arote R, Kim T, Kim Y, Hwang S, Jiang H, Song H, Nah J, Cho M, Cho C: A biodegradable poly (ester amine) based on polycaprolactone and polyethylenimine as a gene carrier. Biomaterials. 2007, 28: 735-744. 10.1016/j.biomaterials.2006.09.028. Seder R, Darrah P, Roederer M: T-cell quality in memory and protection: implications for vaccine design. Nature Reviews Immunology. 2008, 8: 247-258. 10.1038/nri2274. Skowronski D, Astell C, Brunham R, Low D, Petric M, Roper R, Talbot P, Tam T, Babiuk L: Severe acute respiratory syndrome (SARS): a year in review. Annual Review of Medicine. 2005, 56: 357-381. 10.1146/annurev.med.56.091103.134135. Sainz B, Rausch J, Gallaher W, Garry R, Wimley W: Identification and characterization of the putative fusion peptide of the severe acute respiratory syndrome-associated coronavirus spike protein. Journal of Virology. 2005, 79: 7195-7206. 10.1128/JVI.79.11.7195-7206.2005. Wang Z, Yuan Z, Matsumoto M, Hengge U, Chang Y: Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice. Biochemical and Biophysical Research Communications. 2005, 327: 130-135. 10.1016/j.bbrc.2004.11.147. Huang X, Xu J, Qiu C, Ren L, Liu L, Wan Y, Zhang N, Peng H, Shao Y: Mucosal priming with PEI/DNA complex and systemic boosting with recombinant TianTan vaccinia stimulate vigorous mucosal and systemic immune responses. Vaccine. 2007, 25: 2620-2629. 10.1016/j.vaccine.2006.12.020. Jin H, Xiao C, Chen Z, Kang Y, Ma Y, Zhu K, Xie Q, Tu Y, Yu Y, Wang B: Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice. Biochemical and Biophysical Research Communications. 2005, 328: 979-986. 10.1016/j.bbrc.2005.01.048. Garzon Rodrigo M, Berraondo P, Crettaz J, Ochoa L, Vera M, Lasarte J, Vales A, Van Rooijen N, Ruiz J, Prieto J: Induction of gp120-specific protective immune responses by genetic vaccination with linear polyethylenimine-plasmid complex. Vaccine. 2005, 23: 1384-1392. 10.1016/j.vaccine.2004.09.009. Cochran A, Morton D, Stern S, Lana A, Essner R, Wen D: Sentinel lymph nodes show profound downregulation of antigen-presenting cells of the paracortex: implications for tumor biology and treatment. Modern Pathology. 2001, 14: 604-608. 10.1038/modpathol.3880358. Medzhitov R: Recognition of microorganisms and activation of the immune response. Nature. 2007, 449: 819-826. 10.1038/nature06246. Perry A, Gang C, Zheng D, Hong T, Cheng G: The host type I interferon response to viral and bacterial infections. Cell Research. 2005, 15: 407-422. 10.1038/sj.cr.7290309. Son Y, Ahn S, Jang M, Moon Y, Kim S, Cho K, Han S, Yun C: Immunomodulatory effect of resistin in human dendritic cells stimulated with lipoteichoic acid from Staphylococcus aureus. Biochemical and Biophysical Research Communications. 2008, 376: 599-604. 10.1016/j.bbrc.2008.09.037. Wu C, Kirman J, Rotte M, Davey D, Perfetto S, Rhee E, Freidag B, Hill B, Douek D, Seder R: Distinct lineages of Th1 cells have differential capacities for memory cell generation in vivo. Nature immunology. 2002, 3: 852-858. 10.1038/ni832. Aggarwal B: Signalling pathways of the TNF superfamily: a double-edged sword. Nature Reviews Immunology. 2003, 3: 745-756. 10.1038/nri1184. Kim H, Jo E, Park J, Kim J: The enhancing IL-2R alpha mRNA expression induces a marked T cell proliferation with interleukin-2 and anti-CD3 mAb. Korean Journal of Immunology. 1998, 20: 427-434. Kolls J, McCray P, Chan Y: Cytokine-mediated regulation of antimicrobial proteins. Nature Reviews Immunology. 2008, 8: 829-835. 10.1038/nri2433. Kannanganat S, Ibegbu C, Chennareddi L, Robinson H, Amara R: Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells. Journal of Virology. 2007, 81: 8468-8476. 10.1128/JVI.00228-07. Darrah P, Patel D, De Luca P, Lindsay R, Davey D, Flynn B, Hoff S, Andersen P, Reed S, Morris S: Multifunctional Th1 cells define a correlate of vaccine-mediated protection against Leishmania major. Nature Medicine. 2007, 13: 843-850. 10.1038/nm1592. Beloor J, Kang H, Yun C, Kim S, Moon Y: Low lysine treatment increases adipogenic potential of bovine intramuscular preadipocytes. Asian-Australasian Journal of Animal Sciences. 2009, 22: 721-726.