Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses

BMC Immunology - Tập 11 - Trang 1-9 - 2010
Byoung-Shik Shim1,2, Sung-Moo Park3,2, Ji-Shan Quan1,4, Dhananjay Jere1, Hyuk Chu5, Man Ki Song2, Dong Wook Kim2, Yong-Suk Jang6, Moon-Sik Yang6, Seung Hyun Han2,7, Yong-Ho Park3, Chong-Su Cho1, Cheol-Heui Yun1,8
1Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea
2Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea
3Department of Microbiology, College of Veterinary medicine, Seoul National University, Seoul, Republic of Korea
4College of Pharmacy, Yanbian University, China
5Division of Zoonoses, Center for Immunology & Pathology, National Institute of Health, Korea Center for Disease Control & Prevention, Seoul, Republic of Korea
6Division of Biological Sciences and The Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Republic of Korea
7Department of Oral Microbiology & Immunology, Dental Research Institute and BK21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea
8Center for Agricultural Biomaterials, Seoul National University, Seoul, Republic of Korea

Tóm tắt

Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 ± 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220+ cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-Ad) were increased on CD11c+ dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-γ-, TNF-α- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.

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